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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0420 [new locus tag: SAUSA300_RS02250 ]
- pan locus tag?: SAUPAN002146000
- symbol: SAUSA300_0420
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
tslA: type VIIb-secreted phospholipase toxin TslA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0420 [new locus tag: SAUSA300_RS02250 ]
- symbol: SAUSA300_0420
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 472142..473470
- length: 1329
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913617 NCBI
- RefSeq: YP_493133 NCBI
- BioCyc: see SAUSA300_RS02250
- MicrobesOnline: 1291935 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1321ATGTTGAGTAGGAAGTATAAAATAGATTTAAAAGCAATTAATCAAAATACTGAGTCTACA
AGTGCTATTTCGAAAGCGTCATACGAAGTTGAGAATGCTAATAATAATGGTCTATCAAAA
AGAGACGTGATTAATCAATTTAATGATTTGAAAAAAATGAAAAAATTCCCTTCTAATCTT
GAATATGTTGATAGTTACACTGATTCTCTTACTGGAGTAACAACTTCTGCTTTTTTAAAT
AAAGATACAGGCAAAGTAACTCTCGGGATGACTGGGACTAATTTACAAGACGAAGCCTTT
AAAAAGTTAAAAGAAGGTGAATTTTCAAGACAAAATGTTACCAATGCTTTGGAAACAGTT
AAAGATGGATATGCAGATCTTAAAATATTATATTCTCCTGCATCTGATCAAAACTATAGA
TATGCGAATACACAAGAATTTATAAATAAAATAAAAAGTAAGTATGACATTGATTTTATT
ACTGGACATTCACTAGGTGGAAGAGATGCGGTAGTTCTAGGAATGAGTAATGGTATTCCG
AACATTGTGGTTTATAATCCAGCTCCTATTTCTATAACTAGTTTGAATCCTAATTCCCCA
GATGGAAAACGTTTATTAGAATTATATAAAAATTATAAAGGTAATATTACTAGGTTTGTT
GCAGAAAATGATGCATTGACAGAAAATCTGAAGAAATATAAGCATTATGTTTTTTTCGGT
AATGATAAAGTCTTTAAAAATGGTAAAGGTCATGAAATGGAAGGCTTTCTGACCGAAGAA
GAACAAAAAGCTATAAAAAAAGAACTTAAAAAACTACAAGGTTATGCAGAAGAAAATAAT
AAGTCATTTGTAAAGAATTCAAATAATGCTATCTCTAAATTAGCTAGTATAGAATTACTT
AGAGCAAATATGATGACTACAAATGGAGGAGGATTATCTTCTTCGCAGCAAAAAGTTTTA
GAAAGTTTAACAGCTTTAACAATTGCGCAGTCATTCAGTCAACTGATAGACGATGAAATT
AATCAAATCAAAAAAATGTATAATGAAAAGAAAAAGAAATTTGGAAAAAATTGGGAAGAC
GCTCAAAAAGCTGGAAAAGCTGTAGGTGAAGATTTGAGTGTAAATGGAGTTCTCAATGCT
TTAGATGAAGGTCAAGTGAATGAAAGTAGTATGGTAAGAGAACCTGAACAAATGATATCT
GCAAAAGAAAGACAACTTTCAACGATAGGGTCTTCTGTATCAAATTATATTATGAGAGTT
AGACTCAGTATTAATGAAATCGTTGATAAAGATCAAGTGCTTGCATCACAAATAGGTGGA
TTACTATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0420 [new locus tag: SAUSA300_RS02250 ]
- symbol: SAUSA300_0420
- description: hypothetical protein
- length: 442
- theoretical pI: 9.34275
- theoretical MW: 49261.3
- GRAVY: -0.592534
⊟Function[edit | edit source]
- TIGRFAM: Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 11.3)
- TheSEED :
- FIG01107856: hypothetical protein
- PFAM: AB_hydrolase (CL0028) Lipase_3; Lipase (class 3) (PF01764; HMM-score: 15.9)UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 12.8)and 1 moreno clan defined Apo-CIII; Apolipoprotein CIII (Apo-CIII) (PF05778; HMM-score: 10.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.01816
- TAT(Tat/SPI): 0.002573
- LIPO(Sec/SPII): 0.003406
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVINQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKKLKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAEENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQLIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVREPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
- ↑ Stephen R Garrett, Nicole Mietrach, Justin Deme, Alina Bitzer, Yaping Yang, Fatima R Ulhuq, Dorothee Kretschmer, Simon Heilbronner, Terry K Smith, Susan M Lea, Tracy Palmer
A type VII-secreted lipase toxin with reverse domain arrangement.
Nat Commun: 2023, 14(1);8438
[PubMed:38114483] [WorldCat.org] [DOI] (I e)