PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0420 [new locus tag: SAUSA300_RS02250 ]
• pan locus tag^?: SAUPAN002146000
• symbol: SAUSA300_0420
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0420 [new locus tag: SAUSA300_RS02250 ]
• symbol: SAUSA300_0420
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 472142..473470
• length: 1329
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913617 NCBI
• RefSeq: YP_493133 NCBI
• BioCyc: see SAUSA300_RS02250
• MicrobesOnline: 1291935 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  541
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  901
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  1021
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  ATGTTGAGTAGGAAGTATAAAATAGATTTAAAAGCAATTAATCAAAATACTGAGTCTACA
  AGTGCTATTTCGAAAGCGTCATACGAAGTTGAGAATGCTAATAATAATGGTCTATCAAAA
  AGAGACGTGATTAATCAATTTAATGATTTGAAAAAAATGAAAAAATTCCCTTCTAATCTT
  GAATATGTTGATAGTTACACTGATTCTCTTACTGGAGTAACAACTTCTGCTTTTTTAAAT
  AAAGATACAGGCAAAGTAACTCTCGGGATGACTGGGACTAATTTACAAGACGAAGCCTTT
  AAAAAGTTAAAAGAAGGTGAATTTTCAAGACAAAATGTTACCAATGCTTTGGAAACAGTT
  AAAGATGGATATGCAGATCTTAAAATATTATATTCTCCTGCATCTGATCAAAACTATAGA
  TATGCGAATACACAAGAATTTATAAATAAAATAAAAAGTAAGTATGACATTGATTTTATT
  ACTGGACATTCACTAGGTGGAAGAGATGCGGTAGTTCTAGGAATGAGTAATGGTATTCCG
  AACATTGTGGTTTATAATCCAGCTCCTATTTCTATAACTAGTTTGAATCCTAATTCCCCA
  GATGGAAAACGTTTATTAGAATTATATAAAAATTATAAAGGTAATATTACTAGGTTTGTT
  GCAGAAAATGATGCATTGACAGAAAATCTGAAGAAATATAAGCATTATGTTTTTTTCGGT
  AATGATAAAGTCTTTAAAAATGGTAAAGGTCATGAAATGGAAGGCTTTCTGACCGAAGAA
  GAACAAAAAGCTATAAAAAAAGAACTTAAAAAACTACAAGGTTATGCAGAAGAAAATAAT
  AAGTCATTTGTAAAGAATTCAAATAATGCTATCTCTAAATTAGCTAGTATAGAATTACTT
  AGAGCAAATATGATGACTACAAATGGAGGAGGATTATCTTCTTCGCAGCAAAAAGTTTTA
  GAAAGTTTAACAGCTTTAACAATTGCGCAGTCATTCAGTCAACTGATAGACGATGAAATT
  AATCAAATCAAAAAAATGTATAATGAAAAGAAAAAGAAATTTGGAAAAAATTGGGAAGAC
  GCTCAAAAAGCTGGAAAAGCTGTAGGTGAAGATTTGAGTGTAAATGGAGTTCTCAATGCT
  TTAGATGAAGGTCAAGTGAATGAAAGTAGTATGGTAAGAGAACCTGAACAAATGATATCT
  GCAAAAGAAAGACAACTTTCAACGATAGGGTCTTCTGTATCAAATTATATTATGAGAGTT
  AGACTCAGTATTAATGAAATCGTTGATAAAGATCAAGTGCTTGCATCACAAATAGGTGGA
  TTACTATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0420 [new locus tag: SAUSA300_RS02250 ]
• symbol: SAUSA300_0420
• description: hypothetical protein
• length: 442
• theoretical pI: 9.34275
• theoretical MW: 49261.3
• GRAVY: -0.592534

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Degradation esterase, PHB depolymerase family (TIGR01840; EC 3.1.1.-; HMM-score: 11.3)
• TheSEED  :
  • FIG01107856: hypothetical protein
• PFAM:
  AB_hydrolase (CL0028) Lipase_3; Lipase (class 3) (PF01764; HMM-score: 15.9)
  UPF0227; Uncharacterised protein family (UPF0227) (PF05728; HMM-score: 12.8)
  and 1 more
  no clan defined Apo-CIII; Apolipoprotein CIII (Apo-CIII) (PF05778; HMM-score: 10.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01816
  • TAT(Tat/SPI): 0.002573
  • LIPO(Sec/SPII): 0.003406
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162308 NCBI
• RefSeq: YP_493133 NCBI
• UniProt: A0A0H2XKB6 UniProt

⊟Protein sequence

• MLSRKYKIDLKAINQNTESTSAISKASYEVENANNNGLSKRDVINQFNDLKKMKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKKLKEGEFSRQNVTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFITGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRFVAENDALTENLKKYKHYVFFGNDKVFKNGKGHEMEGFLTEEEQKAIKKELKKLQGYAEENNKSFVKNSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQLIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVREPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0419 > SAUSA300_0420 > SAUSA300_0421 > SAUSA300_0422

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications