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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0564 [new locus tag: NWMN_RS03235 ]
  • pan locus tag?: SAUPAN002458000
  • symbol: NWMN_0564
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0564 [new locus tag: NWMN_RS03235 ]
  • symbol: NWMN_0564
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 647403..648023
  • length: 621
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCTATGCGAATCTAAAATCATCAATAAAAACCCCAAATATAGAATTATTAAATATAAT
    GATGAGTACTTAATGATCGATATAATAAGCACTTGGATTAGTTTATTTTTTCCTTTTATT
    AATTGGTTCATCCCCAAAAGATACGTCAAAATTAGTAGAGAAGAGTTTGAAAATTTAAAT
    ATTGTGAAACCTGCTAAAAAGAATGTTTTTTGGCCAGTTGCAGGTATCTCTACTTTATTC
    GCAGTTACATTAAGAAAGTATACACATTTACTTGACACTCAACTTGATAGAAAATTAGTT
    ATTGCTATATGTTGTATCACATTTATAGGGATTTTAACATTTTATGTACGCCTAATTAAA
    AAATCATCTTTAAATATTTATAATACTAAAAATAAAAGGTCAAAAATTATCTTAATACCA
    ACACTAAAAAATTTTTGTTTAACATTGTTTAGATATGCTTTTTTTATTTTATGGACAGTG
    ATTTTCTCATATGCTCTATTATCAATGAGTTATCAAAACATAATAGTATATTTTGCTTGG
    ATTACAGCAATAATGGGTTTTTTTCTAGTGAATATAGCTTTAATTATAGATAAAAACATT
    CATGTCATACTTAAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    621

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0564 [new locus tag: NWMN_RS03235 ]
  • symbol: NWMN_0564
  • description: hypothetical protein
  • length: 206
  • theoretical pI: 10.35
  • theoretical MW: 24436.3
  • GRAVY: 0.534951

Function[edit | edit source]

  • TIGRFAM:
    tandem five-TM protein (TIGR01218; HMM-score: 168.2)
    and 2 more
    CcmD family protein (TIGR04391; HMM-score: 10.9)
    conjugal transfer/type IV secretion protein DotA/TraY (TIGR04346; HMM-score: 10.5)
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined DUF443; Protein of unknown function (DUF443) (PF04276; HMM-score: 211.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002549
    • TAT(Tat/SPI): 0.000111
    • LIPO(Sec/SPII): 0.000391
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MLCESKIINKNPKYRIIKYNDEYLMIDIISTWISLFFPFINWFIPKRYVKISREEFENLNIVKPAKKNVFWPVAGISTLFAVTLRKYTHLLDTQLDRKLVIAICCITFIGILTFYVRLIKKSSLNIYNTKNKRSKIILIPTLKNFCLTLFRYAFFILWTVIFSYALLSMSYQNIIVYFAWITAIMGFFLVNIALIIDKNIHVILKN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]