PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0629 [new locus tag: NWMN_RS03575 ]
• pan locus tag^?: SAUPAN002539000
• symbol: NWMN_0629
• pan gene symbol^?: graS
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0629 [new locus tag: NWMN_RS03575 ]
• symbol: NWMN_0629
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 710043..711083
• length: 1041
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330347 NCBI
• RefSeq: YP_001331663 NCBI
• BioCyc:
• MicrobesOnline: 3706176 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  481
  541
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  1021
  
  ATGAATAATTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGG
  ATATTGTTTTTAAACTTCCTTATGTTAGGCATTAGTCTAATCGATTATGATTTTCCAATA
  GACAGTTTATTTTATATTGTTTCTTTGAATTTAAGTTTAACAATGATTTTTCTTTTATTG
  ACATATTTTAAAGAAGTAAAATTATATAAGCATTTTGACAAAGATAAAGAAATAGAAGAA
  ATTAAACATAAAGATTTAGCGGAAACGCCATTTCAACGTCATACAGTTGATTATTTATAT
  CGTCAAATCTCAGCGCACAAAGAAAAGGTTGTTGAGCAACAGTTGCAATTGAACATGCAT
  GAACAAACCATTACAGAATTTGTGCACGACATAAAAACACCTGTGACAGCTATGAAATTA
  TTAATTGATCAAGAAAAAAATCAAGAAAGAAAACAAGCATTACTATATGAATGGTCTCGT
  ATAAACTCGATGCTAGATACACAGCTGTATATTACTAGATTAGAATCTCAACGTAAAGAT
  ATGTATTTTGATTACGTGTCACTTAAACGCATGGTCATTGATGAAATACAATTAACAAGA
  CATATTAGTCAGGTTAAAGGTATTGGTTTTGATGTTGACTTTAAAGTGGATGATTATGTT
  TATACAGATATAAAATGGTGTCGTATGATTATTAGACAAATTTTGTCAAACGCATTGAAA
  TATAGTGAGAATTTTAATATTGAAATTGGGACAGAATTAAATGATCAACATGTTTCGTTA
  TATATTAAAGACTATGGCAGAGGTATTAGTAAAAAAGATATGCCGCGAATATTTGAACGA
  GGATTTACGTCAACGGCTAACAGAAATGAAACGACGTCTTCAGGTATGGGTCTATATTTA
  GTAAATAGTGTAAAGGATCAATTAGGTATTCACCTGCAAGTCACGTCGACTGTTGGTAAG
  GGGACAACTGTCAGATTGATTTTCCCATTACAAAATGAAATTGTTGAACGCATGTCGGAA
  GTGACAAATTTGTCATTTTAA
  60
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  1041
  

⊟Protein

⊟General

• locus tag: NWMN_0629 [new locus tag: NWMN_RS03575 ]
• symbol: NWMN_0629
• description: hypothetical protein
• length: 346
• theoretical pI: 7.34071
• theoretical MW: 41078.2
• GRAVY: -0.238439

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 89.1)
  and 10 more
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 66)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 60.5)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.1)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.1)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 25.3)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 25.3)
  Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.2)
  Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.2)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 14.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 13.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 69.2)
  and 3 more
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.4)
  HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 17.4)
  no clan defined TA0956; Thermoplasma acidophilum protein TA0956 (PF11513; HMM-score: 14.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007904
  • TAT(Tat/SPI): 0.000137
  • LIPO(Sec/SPII): 0.043566
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 151220841 NCBI
• RefSeq: YP_001331663 NCBI
• UniProt: A6QEW9 UniProt

⊟Protein sequence

• MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0627 > NWMN_0628 > NWMN_0629

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications