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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0640 [new locus tag: NWMN_RS03635 ]
- pan locus tag?: SAUPAN002553000
- symbol: NWMN_0640
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0640 [new locus tag: NWMN_RS03635 ]
- symbol: NWMN_0640
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 723706..724350
- length: 645
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330356 NCBI
- RefSeq: YP_001331674 NCBI
- BioCyc:
- MicrobesOnline: 3706187 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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601GTGAAAGTAAAGTATATAGATAAACGTCACTGGCGTCGCCTAATTGATAGGGAATACACA
GAGGTAAAAGTTAATAATAATAGGTTTAAGGGTATTATAGGCTTAGTCACGATGAAAAAG
GTTCGTGATCCTTTAGAGGTGACGGTAGTTGGACAAAATATCATTGTCGCAGATGACAAT
TATAAATGGTTGCAAATACTACCTGAAAAGAAACGTTATAGTATAACTGTAATGTTTGAT
AATAAAGGCAATCCATTAGAATATTATTTTGATATAAATATCAAAAATATAACGCAAAAA
GGTAATGCGTGTACAGTAGATTTATGTTTAGATGTTTTAGCATTACCAAGTGGTGAATAT
GAGTTGGTAGATGAAGATGATTTAATGTTTGCATTAGAAAGTGAGCAAATTACAAAAAAG
CAATTTCATGAAGCATATATGATTGCACATCAAATTATGGCAGAGTTAGAAAATGATTTT
AAAGGATTCCAAAAGAAAATCATGTACTGCTTTAATAAAATTAATGCAAAGGCTCAAAAA
AATCATCAAAAGCCACAAAATAAAACTAATATTGAAAAAAGCAAACAAATAAAGCCTAAG
CAATATAATCAAACTAAAAATCACCAACAACAAAAGAAAAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0640 [new locus tag: NWMN_RS03635 ]
- symbol: NWMN_0640
- description: hypothetical protein
- length: 214
- theoretical pI: 9.81099
- theoretical MW: 25363.2
- GRAVY: -0.794393
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: no clan defined DUF402; Protein of unknown function (DUF402) (PF04167; HMM-score: 60.3)and 2 moreSAS-6_N; Centriolar protein SAS N-terminal (PF16531; HMM-score: 14.5)ABC_transp_aux; ABC-type uncharacterized transport system (PF09822; HMM-score: 9.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003042
- TAT(Tat/SPI): 0.000171
- LIPO(Sec/SPII): 0.000447
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDPLEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGNACTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDFKGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
NWMN_1574 (infC) translation initiation factor IF-3 [1] (data from MRSA252) NWMN_2152 (rplC) 50S ribosomal protein L3 [1] (data from MRSA252) NWMN_2151 (rplD) 50S ribosomal protein L4 [1] (data from MRSA252) NWMN_2137 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) NWMN_2145 (rplP) 50S ribosomal protein L16 [1] (data from MRSA252) NWMN_1151 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) NWMN_2147 (rplV) 50S ribosomal protein L22 [1] (data from MRSA252) NWMN_2150 (rplW) 50S ribosomal protein L23 [1] (data from MRSA252) NWMN_1613 (rpsD) 30S ribosomal protein S4 [1] (data from MRSA252) NWMN_2119 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) NWMN_2143 (rpsQ) 30S ribosomal protein S17 [1] (data from MRSA252) NWMN_0249 5'-nucleotidase, lipoprotein e(P4) family protein [1] (data from MRSA252) NWMN_1382 DNA-binding protein HU [1] (data from MRSA252) NWMN_1600 universal stress protein family protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)