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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0659 [new locus tag: NWMN_RS03735 ]
- pan locus tag?: SAUPAN002574000
- symbol: NWMN_0659
- pan gene symbol?: —
- synonym:
- product: anion transporter family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_0659 [new locus tag: NWMN_RS03735 ]
- symbol: NWMN_0659
- product: anion transporter family protein
- replicon: chromosome
- strand: +
- coordinates: 739969..741522
- length: 1554
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5330374 NCBI
- RefSeq: YP_001331693 NCBI
- BioCyc:
- MicrobesOnline: 3706206 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501ATGTCAGAAGAAAAACATGTAGTTGAACATGAACAACAAAAGAAAGAAAAGACAAAAAAG
CAATACAAGCCATTTTGGATTGTCATGAGTTTTATAATACTTATAGTTGTACTATTACTC
CCGGCACCTTCAAGTCTGCCGATAATGGCTAAGGCAGTACTAGCTATTTTAGCTTTTGCA
GTTATTATGTGGGTAACGGAAGCTGTATCATATCCGGTGTCAGCAACTTTAATTATTGGC
TTAATGATATTACTTTTAGGATTTAGCCCTGTTCAAAATTTAGGGGAGAAGCTAGGTAAT
CCGAAAAGTGGCAGTGCTATTTTAGCTGGAAGTGACCTTCTAGGAACTAATCATGCATTA
TCATTAGCGTTTAGTGGATTTGCAACTTCAGCTGTAGCTCTCGTTGCAGCTGCATTATTT
TTGGCTGCTGCTATGCAAGAAACGAATTTGCATAAAAGACTAGCTCTTTTAGTGTTATCA
ATTGTTGGTAATAAAACTAGAAATATAGTTATTGGAGCAATTATCGTTTCAATTGTACTT
GCATTTTTCGTTCCTTCTGCAACAGCTAGAGCAGGGGCAGTTGTACCAATCTTGCTGGGT
ATGATTGCGGCATTTAAAGTTTCCAAAGATAGCAAGTTAGCGTCTTTATTAATAATTACT
TCAGTACAAGCTGTGTCAATTTGGAATATTGGTATCAAAACGGCGGCAGCACAAAATATC
GTAGCGATTAATTTTATAAACCATCAATTAGGATTTGATGTTTCATGGGGCGAGTGGTTC
TTATATGCAGCGCCTTGGTCCATAGTTATGTCCGTAGCTTTATATTTCATCATGATTAAA
GTGATGCCTCCAGAAATTAATACAATAGAAGGTGGTAAAGATTTAATAAAAGAAGAATTG
CATAAACTTGGCCCCGTTAGCCCACGTGAATGGCGTTTAATTGTTATATCGATGTTATTA
TTACTGTTTTGGTCAACTGAAAAAGTATTACATCCGATTGACTCTGCATCCATTACTATT
ATTGCTTTAGGTGTTATGTTAATGCCGAAAATTGGTGTCATGACATGGAAACATGTTGAA
AATAAAATACCATGGGGAACAATTATCGTGTTTGGTGTAGGTATTTCACTAGGTAACGTT
CTTTTGAAAACAGGTGCAGCTCAATGGTTAAGTGATCAAACTTTTGGTGTTTTAGGTTTA
AAACATTTACCTATTATCGCGACAATTGCACTTATCACGCTTTTTAATATATTGATTCAT
TTGGGCTTTGCGAGTGCAACAAGTTTATCATCAGCGTTAATACCTGTTTTTATTTCGCTA
ACCTCTACGTTACACTTAGGAGACCAGTCTATAGGATTTGTTTTAATTCAACAATTTGTT
ATTAGTTTTGGTTTCTTATTACCTGTTAGTGCACCTCAAAATATGTTGGCTTATGGCACT
GGTACTTTTACGGTTAAAGATTTCTTGAAGGCAGGTATACCATTGACAATTGTAGGGTAT
ATTCTAGTGATAGTTTTTAGCATGACTTATTGGAAATGGTTAGGTTTGCTTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_0659 [new locus tag: NWMN_RS03735 ]
- symbol: NWMN_0659
- description: anion transporter family protein
- length: 517
- theoretical pI: 10.1501
- theoretical MW: 55863.7
- GRAVY: 0.889168
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Cations and iron carrying compounds transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257.2)Transport and binding proteins Anions transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257.2)and 1 moreTIGR03943 family protein (TIGR03943; HMM-score: 5.6)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: IT (CL0182) Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 367.3)and 1 moreCitMHS; Citrate transporter (PF03600; HMM-score: 34.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 11
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015225
- TAT(Tat/SPI): 0.000673
- LIPO(Sec/SPII): 0.00453
- predicted transmembrane helices (TMHMM): 12
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPSSLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPKSGSAILAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLSIVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLIITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYFIMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPIDSASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSDQTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQSIGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSMTYWKWLGLL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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