PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0645 [new locus tag: SA_RS03685 ]
• pan locus tag^?: SAUPAN002574000
• symbol: SA0645
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0645 [new locus tag: SA_RS03685 ]
• symbol: SA0645
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 738629..740182
• length: 1554
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123452 NCBI
• RefSeq: NP_373900 NCBI
• BioCyc: see SA_RS03685
• MicrobesOnline: 102926 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGTCAGAAGAAAAACATGTAGTGGAACATGAACAACAAAAGAAAGAAAAGACAAAAAAG
  CAATACAAGCCATTTTGGATTGTCATGAGTTTTATAATACTTATAGTTGTACTATTACTC
  CCGGCACCTTCAAGTCTGCCGATAATGGCTAAGGCAGTACTAGCTATTTTAGCTTTTGCA
  GTTATTATGTGGGTAACGGAAGCTGTATCATATCCGGTGTCAGCAACTTTAATTATTGGC
  TTAATGATATTACTTTTAGGATTTAGCCCGGTTCAAAATTTAGGGGAGAAGCTAGGTAAT
  CCGAAAAGTGGCAGTGCTATTTTAGCTGGAAGTGACCTTCTAGGAACTAATCATGCATTA
  TCATTAGCGTTTAGTGGATATGCAACTTCAGCTGTAGCTCTCGTTGCAGCTGCATTATTT
  TTGGCTGCTGCTATGCAAGAAACGAATTTGCATAAAAGACTAGCTCTTTTAGTGTTATCA
  ATTGTTGGTAATAAAACTAGAAATATAGTTATTGGAGCAATTATCGTTTCAATTGTACTT
  GCATTTTTCGTTCCTTCTGCAACAGCTAGAGCAGGGGCAGTTGTACCAATCTTGCTGGGT
  ATGATTGCGGCATTTAAAGTTTCCAAAGATAGCAAGTTAGCGTCTTTATTAATAATTACT
  TCAGTACAAGCTGTGTCAATTTGGAATATTGGTATCAAAACGGCGGCAGCACAAAATATC
  GTAGCGATTAATTTTATAAACCATCAATTAGGATTTGATGTTTCATGGGGCGAGTGGTTC
  TTATATGCAGCGCCTTGGTCCATAGTTATGTCCGTAGCTTTATATTTCATCATGATTAAA
  GTGATGCCTCCAGAAATTAATACAATAGAAGGTGGTAAAGATTTAATAAAAGAAGAATTG
  CATAAACTTGGCCCCGTTAGCCCACGTGAATGGCGTTTAATTGTTATATCGATGTTATTA
  TTACTGTTTTGGTCAACTGAAAAAGTATTACATCCGATTGACTCTGCATCCATTACTATT
  ATTGCTTTAGGTGTTATGTTAATGCCGAAAATTGGTGTCATGACATGGAAACATGTTGAA
  AATAAAATACCATGGGGAACAATTATCGTGTTTGGTGTAGGTATTTCACTAGGGAACGTT
  CTTTTGAAAACAGGTGCAGCTCAATGGTTAAGTGATCAAACTTTTGGTGTTTTAGGTTTA
  AAACATTTACCTATTATCGCGACAATTGCACTTATCACGCTTTTTAATATATTGATTCAT
  TTGGGCTTTGCGAGTGCAACAAGTTTATCATCAGCGTTAATACCTGTTTTTATTTCGCTA
  ACCTCTACGTTACACTTAGGAGACCAGTCTATAGGATTTGTTTTAATTCAACAATTTGTT
  ATTAGTTTTGGTTTCTTATTACCTGTTAGTGCACCTCAAAATATGTTGGCTTATGGCACT
  GGTACTTTTACGGTTAAAGATTTCTTGAAGGCAGGTATACCATTGACAATTGTAGGGTAT
  ATTCTAGTGATAGTTTTTAGTATGACTTATTGGAAATGGTTAGGTTTGCTTTAA
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  1554
  

⊟Protein

⊟General

• locus tag: SA0645 [new locus tag: SA_RS03685 ]
• symbol: SA0645
• description: hypothetical protein
• length: 517
• theoretical pI: 10.1134
• theoretical MW: 55879.7
• GRAVY: 0.881238

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257)
  Metabolism Transport and binding proteins Anions transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 257)
  and 1 more
  TIGR03943 family protein (TIGR03943; HMM-score: 6.1)
• TheSEED  :
  • Sodium/di- and tricarboxylate cotransporter
• PFAM:
  IT (CL0182) Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 367.8)
  and 1 more
  CitMHS; Citrate transporter (PF03600; HMM-score: 33.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 11
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.015225
  • TAT(Tat/SPI): 0.000673
  • LIPO(Sec/SPII): 0.00453
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15926367 NCBI
• RefSeq: NP_373900 NCBI
• UniProt: A0A0H3JTB1 UniProt

⊟Protein sequence

• MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPSSLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPKSGSAILAGSDLLGTNHALSLAFSGYATSAVALVAAALFLAAAMQETNLHKRLALLVLSIVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLIITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYFIMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPIDSASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSDQTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQSIGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSMTYWKWLGLL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications