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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0676 [new locus tag: NWMN_RS03825 ]
  • pan locus tag?: SAUPAN002593000
  • symbol: NWMN_0676
  • pan gene symbol?: saeQ
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0676 [new locus tag: NWMN_RS03825 ]
  • symbol: NWMN_0676
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 758336..758809
  • length: 474
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATAAATTATTGCTACTCGTTACATTTATCATTCGTGTGGGTTCAGGTATTGTTATG
    TTAATGCAAGGCTACGAAAAATTAACGGGCGGATTTACGCTGAAAGGTTTAGTACCAGTC
    ATCGCTAACAATACTGATTCACCAGAGTGGTATAAGTGGTTTTTCGCAAATATAGTTGCA
    CATACGACGTCATTATTTGATATTGTTGTCCCACTCGGAGAGATTGCAATTGGATTAGGT
    TTAATTTTTGGAGTTTTTGCATATGCTGCTAGTTTCTTTGGAGCCTTTGTTATGATAAAT
    TATATCTTAGCAGATATGATATTTACGTATCCTCTTCAATTAACTTTCTTTATCCTTTTA
    CTAATGAGTCACTCATTGTTAAAACAGATTTCACTTAAAGAAATCATTAATTACTTTAGA
    GGTCGTAAGAACAGAGGTGAAAAAATAGATGACCCACTTACTGATCGTGGATGA
    60
    120
    180
    240
    300
    360
    420
    474

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0676 [new locus tag: NWMN_RS03825 ]
  • symbol: NWMN_0676
  • description: hypothetical protein
  • length: 157
  • theoretical pI: 9.33884
  • theoretical MW: 17664.9
  • GRAVY: 0.638217

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    DoxD-like (CL0131) DoxX; DoxX (PF07681; HMM-score: 35.5)
    and 2 more
    no clan defined Oxidored_q2; NADH-ubiquinone/plastoquinone oxidoreductase chain 4L (PF00420; HMM-score: 12.6)
    NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 7.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.140655
    • TAT(Tat/SPI): 0.001065
    • LIPO(Sec/SPII): 0.094279
  • predicted transmembrane helices (TMHMM): 3

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANNTDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYILADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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