PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0683 [new locus tag: NWMN_RS03860 ]
• pan locus tag^?: SAUPAN002600000
• symbol: pabB
• pan gene symbol^?: pabB
• synonym:
• product: anthranilate/para-aminobenzoate synthase component I

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0683 [new locus tag: NWMN_RS03860 ]
• symbol: pabB
• product: anthranilate/para-aminobenzoate synthase component I
• replicon: chromosome
• strand: +
• coordinates: 763288..764439
• length: 1152
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332233 NCBI
• RefSeq: YP_001331717 NCBI
• BioCyc:
• MicrobesOnline: 3706230 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGAGAATAGAATATAATTATCGCTACTATTTAACTGAAAATGAATATAAGCAATACCAT
  ATTCAATTAAAGGGATTTATAAAGAAGTATGTTGCTACTAAGTTGGCTGATGTGGGAGAA
  GTGATACACTTTGCACAAGCGCAGCAACGACAAGGTAGATATGTCTCGTTATATTTAAGT
  TACGAAGCGGCAAAGTATTTTAATCATGCTATGTGTACACATTCATTAGCTAAAGATGAT
  ATTTATGCAGCAGCTTATAGTTTTGAAAAAGCGGAAAGCATAAATTCAACATATGAACAT
  CAAACTTCTTATGTATCAAAGTATCATTTTTCATTTGTTGAATCTTCTGAGGTTATGATG
  ACTAATATTAAACGTGTCCAACAAGCAATTGTTGAAGGCGAAACGTATCAAGTGAACTAT
  ACGGCGCGCTTAACGGATAACATTTATTATCCTATTAGTACTTTATATGAACGATTAACT
  CAATTTAGTAATGGTAATTATACTGCGTTATTACAAACTGATGAAATCCAAGTAGCGTCT
  ATCTCACCAGAATTATTTTTTCAAAAAGGACAATTTAACAATGTCGATAACGTTATCATA
  AGCAAACCGATGAAAGGGACAATGCCTAGAGGTAAAACGGAAGCTGAAGATCAACAGTAT
  TATAAAACATTGCAAACTTCTTCGAAAGATCGTGCAGAAAATGTCATGATTGTTGATTTA
  CTAAGAAACGATATAGGGAGAATATCACAGAGTGGCTCAATTAAGGTGTATAAACTATTT
  TTTATTGAGGCATATAAAACTGTATTTCAAATGACTTCGATGGTAAGTGGAACTTTAAAA
  AATAATACAGACTTAACTCAAATTTTAACATCGTTATTTCCTTGTGGTTCGATTACAGGT
  GCACCGAAACTGAATACAATGAAATATATTAAACAATTAGAAAGTTCACCTCGTGGTATA
  TACTGCGGAGCAATTGGACTATTACTTCCAACTGAAGATGATAAAATGATTTTTAATATT
  CCGATTCGCACTATTGAGTATAAATATGGACAAGCGATTTATGGAGTCGGAGCAGGTATT
  ACAATTGATTCTAAGCCAAAAGATGAAGTGAATGAATTTTACGCAAAAACCAAGATTTTG
  GAGATGTTATAA
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⊟Protein

⊟General

• locus tag: NWMN_0683 [new locus tag: NWMN_RS03860 ]
• symbol: PabB
• description: anthranilate/para-aminobenzoate synthase component I
• length: 383
• theoretical pI: 8.30263
• theoretical MW: 43964.8
• GRAVY: -0.358225

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 328.3)
  aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 270.6)
  and 8 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 223.7)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 207.7)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 140.4)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 128.6)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 105.5)
  salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 96.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone isochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 93)
  Arabidopsis thaliana cystatin-related protein (TIGR01638; HMM-score: 13)
• TheSEED: data available for N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined Chorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 270.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008779
  • TAT(Tat/SPI): 0.00043
  • LIPO(Sec/SPII): 0.000798
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220895 NCBI
• RefSeq: YP_001331717 NCBI
• UniProt: A0A0H3K7H3 UniProt

⊟Protein sequence

• MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHFAQAQQRQGRYVSLYLSYEAAKYFNHAMCTHSLAKDDIYAAAYSFEKAESINSTYEHQTSYVSKYHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLTQFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQQYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVSGTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDDKMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: pabA > pabB > pabC

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications