PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0692 [new locus tag: NWMN_RS03915 ]
• pan locus tag^?: SAUPAN002611000
• symbol: hisC
• pan gene symbol^?: hisC
• synonym:
• product: histidinol-phosphate aminotransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0692 [new locus tag: NWMN_RS03915 ]
• symbol: hisC
• product: histidinol-phosphate aminotransferase
• replicon: chromosome
• strand: +
• coordinates: 776412..777470
• length: 1059
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330397 NCBI
• RefSeq: YP_001331726 NCBI
• BioCyc:
• MicrobesOnline: 3706239 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  1021
  
  ATGAAAGAACAACTTAATCAACTATCAGCATATCAGCCTGGTTTATCTCCAAGGGCATTG
  AAAGAAAAGTATGGCATTGAAGGAGATTTATATAAACTTGCATCAAATGAAAATTTGTAT
  GGACCATCGCCTAAAGTTAAAGAAGCGATATCAGCACACTTAGATGAGTTATATTATTAT
  CCTGAAACAGGATCACCGACATTAAAAGCGGCGATTAGTAAACATTTAAATGTAGATCAA
  TCACGCATTTTATTTGGTGCGGGATTAGATGAAGTTATATTAATGATTTCTAGAGCTGTA
  TTAACGCCAGGGGATACTATTGTTACAAGTGAAGCGACATTCGGTCAATATTATCACAAT
  GCGATTGTTGAATCAGCTAATGTGATACAAGTACCTTTAAAAGATGGTGGCTTCGATTTA
  GAAGGTATTTTAAAAGAAGTTAATGAAGATACGTCATTGGTATGGTTATGTAATCCAAAT
  AATCCTACAGGTACATATTTTAATCATGAGAGCTTAGATTCGTTTTTATCTCAAGTACCT
  CCACATGTACCAGTAATTATAGATGAAGCTTATTTTGAATTTGTGACAGCAGAGGACTAC
  CCGGATACACTTGCTTTGCAACAAAAATATGACAATGCTTTCTTATTACGTACATTTTCA
  AAGGCGTATGGATTAGCGGGTTTACGTGTAGGATATGTGGTAGCAAGTGAACATGCGATT
  GAAAAATGGAACATCATTAGACCACCATTTAATGTGACACGTATATCTGAATACGCAGCA
  GTTGCAGCACTTGAAGATCAACAATATTTAAAAGAGGTAACACATAAAAATAGTGTTGAA
  CGCGAAAGATTTTATCAATTACCTCAAAGTGAGTATTTCTTGCCAAGTCAAACGAATTTT
  ATATTTGTAAAAACAAAGCGGGTAAATGAACTTTATGAAGCACTTTTAAATGTAGGGTGT
  ATTACGCGACCATTTCCAACTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGAT
  AAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAG
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⊟Protein

⊟General

• locus tag: NWMN_0692 [new locus tag: NWMN_RS03915 ]
• symbol: HisC
• description: histidinol-phosphate aminotransferase
• length: 352
• theoretical pI: 4.84534
• theoretical MW: 39787.8
• GRAVY: -0.258239

⊟Function

• reaction:
  EC 2.6.1.9?  ExPASy
  Histidinol-phosphate transaminase L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 305.7)
  and 15 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 182)
  Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 77.6)
  beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 62.1)
  LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 60.9)
  succinyldiaminopimelate transaminase (TIGR03538; EC 2.6.1.17; HMM-score: 59.1)
  succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 58.7)
  putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 53.1)
  LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 50.4)
  tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 49.6)
  succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 44.8)
  Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 37.7)
  enduracididine biosynthesis enzyme MppP (TIGR04462; EC 2.-.-.-; HMM-score: 24.6)
  enduracididine biosynthesis enzyme MppQ (TIGR04461; EC 2.-.-.-; HMM-score: 22)
  Metabolism Energy metabolism Amino acids and amines aspartate 4-decarboxylase (TIGR03801; EC 4.1.1.12; HMM-score: 19.9)
  cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 18.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 167.5)
  and 4 more
  Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 20.6)
  Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 18.8)
  Aminotran_MocR; Alanine-glyoxylate amino-transferase (PF12897; HMM-score: 17.4)
  Alliinase_C; Allinase (PF04864; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.01701
  • TAT(Tat/SPI): 0.001827
  • LIPO(Sec/SPII): 0.001818
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220904 NCBI
• RefSeq: YP_001331726 NCBI
• UniProt: A6QF32 UniProt

⊟Protein sequence

• MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSPKVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLTPGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPNNPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRTFSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHKNSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIGFKEQNDKMLEVLSNFKYE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications