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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0709 [new locus tag: NWMN_RS04015 ]
  • pan locus tag?: SAUPAN002645000
  • symbol: NWMN_0709
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0709 [new locus tag: NWMN_RS04015 ]
  • symbol: NWMN_0709
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 794682..795560
  • length: 879
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAAAATTGTTATTATCGCTGTTTTAGCGATTTTATTTGTAGTAATAAGTGCTTGT
    GGTAATAAAGAAAAAGAGGCACAACATCAATTTACTAAGCAATTTAAAGATGTTGAGCAA
    AAACAAAAAGAATTACAACATGTCATGGATAATATACATTTGAAAGAAATTGATCATCTA
    AGTAAAACTGATACAACTGATAAAAATAGTAAAGAATTTAAGGCACTACAAGAAGATGTT
    AAAAACCATCTCATACCTAAATTTGAAGCATATTATAAGTCAGCAAAAAATTTGCCTGAT
    GATACAATGAAAGTTAAGAAATTAAAAAAAGAATATATGACGCTTGCAAATGAGAAGAAG
    GATGCGATATATCAATTAAAAAAATTCATAGGTTTATGTAATCAATCTATCAAGTATAAC
    GAAGACATTTTAGATTATACGAAACAATTTGAAAAAAATAGATACAAAGTTGAATCAGAA
    ATTAAATTAGCTGATAATAAAAGTGAAGCAACTAATCTTACGACAAAATTAGAACATAAT
    AATAAAGCGTTAAGAGATACTGCGAAGAAGAACCTAGATGATAGTAAAGAAAATGAAGTA
    AAAGGCGCGATTAAAAATCACATTATGCCAATGATTGAAAAGCAAATTACCGATATTAAC
    CAAACTAATATTAGTGATAAGCATGTTAATAATGCAAGGAAAAACGCAATAGAAATGTAT
    TACAGTCTGCAGAACTATTATAATACACGTATTGAAACAATAAAGGTTAGTGAGAAGTTA
    TCAAAAGTCGATGTAGATAAGTTGCCGAAAAAGGGTATAGATATAACTCACGGCGATAAA
    GCCTTTGAAAAAAAGCTTGAAAAATTAGAAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0709 [new locus tag: NWMN_RS04015 ]
  • symbol: NWMN_0709
  • description: hypothetical protein
  • length: 292
  • theoretical pI: 9.71132
  • theoretical MW: 34082
  • GRAVY: -0.89726

Function[edit | edit source]

  • TIGRFAM:
    WXG100 family type VII secretion target (TIGR03930; HMM-score: 9.2)
    and 3 more
    Cellular processes Cellular processes Cell division cell division protein FtsL (TIGR02209; HMM-score: 7.2)
    phage lysis regulatory protein, LysB family (TIGR03495; HMM-score: 6.8)
    Cellular processes Cellular processes Sporulation and germination stage II sporulation protein R (TIGR02837; HMM-score: 4.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined IncA; IncA protein (PF04156; HMM-score: 16.3)
    Ca_hom_mod; Calcium homeostasis modulator (PF14798; HMM-score: 14.4)
    4HB_MCP (CL0457) TarH; Tar ligand binding domain homologue (PF02203; HMM-score: 13.3)
    no clan defined DMPK_coil; DMPK coiled coil domain like (PF08826; HMM-score: 13.3)
    and 2 more
    DUF3008; Protein of unknwon function (DUF3008) (PF11450; HMM-score: 12.3)
    Ac76; Orf76 (Ac76) (PF05814; HMM-score: 7.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helix: 1
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: VVISACG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.000634
    • TAT(Tat/SPI): 0.000051
    • LIPO(Sec/SPII): 0.999117
    • Cleavage Site: CS pos: 19-20. ISA-CG. Pr: 0.9997
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKELQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDTMKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESEIKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITDINQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDITHGDKAFEKKLEKLEEK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]