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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0737 [new locus tag: NWMN_RS04180 ]
  • pan locus tag?: SAUPAN002697000
  • symbol: NWMN_0737
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0737 [new locus tag: NWMN_RS04180 ]
  • symbol: NWMN_0737
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 829895..830797
  • length: 903
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAACAATACTTAATTACTGGTGGGACTGGTATGGTTGGATCTCAATTAGTTAATGAA
    ATTAAAAAATCAGATTCACATATCACGATATTAACGCGACACGACCAAATTTCAAATGAT
    AAGAAAATTTCATATGTCAACTGGGCTAAATCTGGGTGGGAACACAAAGTTCCTCAAAAT
    ATCGATGTGGTCATCAACTTAGCAGGTGCTACATTGAATAAACGATGGACACCGGAATAT
    AAACAAACGCTAATGCTAAGTAGAATTCAATCTACGCAAGCTTTATATGAATTGTTTAAA
    TCACGTAATAAAGCACCTAAAGCTTTATTTAATGCTAGTGCCACTGGTTACTATCCTCCT
    GATTTATTTATGAGTTACACAGAAGTTTATAAAACTTTACCTTTTGATTTCTTATCAGAT
    ATTGTGTATCAATGGGAACGTTTCGCACAACAATTTGAACAATTAGGTACACGAGTTGTC
    ATCGGTCGTTTCGGTATAATTTTATCGAATGAAGGCGGTGCGTTACAAACAATGAAACTA
    CCATACGAATATTACATTGGTGGTAAATTAGGTTCTGGTCAACAATGGTATTCATGGATT
    CATATCAATGATTTAATTCAAGCTATTTTATTTTTAATAAATAACGAGTCAGCTAGTGGT
    CCGTTTAATTTAACTGCACCTATACCTGAACGTCAAAATTTATTTGGCTACACTTTAGCA
    AGAGCTATGCATAAGCCTCATGAAACTTGGGCACCAAGTCTTGCAATGCGTCTCATACTT
    GGTCAAATGTCAACAGTAGTATTGGATACTCAAAAAGTATTACCTAATAAAATTCAAGCA
    TTGGGATTCCAATTTAAATATAGTAATTTAAAAATGGCACTTGAAGATTTAATTAAAGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_0737 [new locus tag: NWMN_RS04180 ]
  • symbol: NWMN_0737
  • description: hypothetical protein
  • length: 300
  • theoretical pI: 9.72292
  • theoretical MW: 34227.2
  • GRAVY: -0.227

⊟Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 361.9)
    and 5 more
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 29.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 20.2)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.5)
    Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 14.3)
    thioester reductase domain (TIGR01746; HMM-score: 12.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 67.6)
    no clan defined DUF1731; Domain of unknown function (DUF1731) (PF08338; HMM-score: 62)
    and 8 more
    NADP_Rossmann (CL0063) NAD_binding_4; Male sterility protein (PF07993; HMM-score: 24.9)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 20.7)
    KR; KR domain (PF08659; HMM-score: 18)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 15.5)
    Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 14.4)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 14.3)
    NmrA; NmrA-like family (PF05368; HMM-score: 12.9)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.029732
    • TAT(Tat/SPI): 0.000589
    • LIPO(Sec/SPII): 0.005143
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKISYVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSRNKAPKALFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVVIGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESASGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPNKIQALGFQFKYSNLKMALEDLIKE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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