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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0773 [new locus tag: NWMN_RS04370 ]
  • pan locus tag?: SAUPAN002818000
  • symbol: NWMN_0773
  • pan gene symbol?: ntrA
  • synonym:
  • product: nitroreductase family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0773 [new locus tag: NWMN_RS04370 ]
  • symbol: NWMN_0773
  • product: nitroreductase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 865139..865678
  • length: 540
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGGAATTACAACAAGCAATAGCTAATAGAAGAAGTGTGAAAAAATTTAAAAGAGATATG
    CACATAGATGACGCATTGCTATATCAAGCAATTGAGAAAGCTGCTGATGCTCCAAATCAC
    GGAATGAGGGAACCATGGAGAGTTGTGCATGTTCCGAAAGACAGATTAGGAGATATGAGT
    AAGGATATTTCTAAATTTGCATTTCCTAATGAATTAGATAAGCAACAATGTCATTATGAT
    GCAGTTACGAAACTAGGTGGCATGTTATTGCTTATTTTAAAAACAGATCCAAGACAACGT
    CAAAATGATGAAAACTACTTTGCATTTGGTGCATATGCACAAAATCTTATGTTGTTACTT
    TATGAAGCGGGAATAGGTACATGTTGGAAATCGCCATTATATATCTATGATCCTAAAGTA
    AGAAAAACACTTGGTATAAAGAAAGATGAAGTTCTTGCTGGATTCTTATATTTAACGGAT
    TTAGAAGGAGATATGCCTAAAGCACCACGTAAAAATAGAAACTTAATTACATTATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0773 [new locus tag: NWMN_RS04370 ]
  • symbol: NWMN_0773
  • description: nitroreductase family protein
  • length: 179
  • theoretical pI: 9.4401
  • theoretical MW: 20676.9
  • GRAVY: -0.496089

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other F420 biosynthesis protein FbiB, C-terminal domain (TIGR03553; HMM-score: 33)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5,6-dimethylbenzimidazole synthase (TIGR02476; EC 1.14.99.40; HMM-score: 32.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    FMN-dep-NRtase (CL0529) Nitroreductase; Nitroreductase family (PF00881; HMM-score: 78.7)
    and 1 more
    TM1586_NiRdase; Putative TM nitroreductase (PF14512; HMM-score: 23.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00342
    • TAT(Tat/SPI): 0.000401
    • LIPO(Sec/SPII): 0.000649
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMREPWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQNDENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTDLEGDMPKAPRKNRNLITLY

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]