PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0826 [new locus tag: NWMN_RS04665 ]
• pan locus tag^?: SAUPAN003058000
• symbol: NWMN_0826
• pan gene symbol^?: —
• synonym:
• product: NADH-dependent flavin oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0826 [new locus tag: NWMN_RS04665 ]
• symbol: NWMN_0826
• product: NADH-dependent flavin oxidoreductase
• replicon: chromosome
• strand: +
• coordinates: 913067..914194
• length: 1128
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330486 NCBI
• RefSeq: YP_001331860 NCBI
• BioCyc:
• MicrobesOnline: 3706375 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAAGTAAATACGAACCATTGTTTGATAAAGTAGAATTACCAAATGGAGTAGAGTTG
  AGAAATCGATTTGTGTTAGCCCCTTTAACACATATTTCTTCAAATGATGATGGTACTATT
  TCAGATGTAGAACTTCCTTATATTGAAAAGCGTTCACAAGATGTTGGTATTACAATTAAT
  GCTGCGAGTAATGTGAGTGATGTCGGAAAAGCATTTCCAGGACAGCCATCAATCGCGCAT
  GACAGTAATATTGAAGGACTAAAACGATTAGCTACAGCAATGAAGAAAAACGGTGCCAAA
  GCACTCGTACAAATACATCATGGCGGTGCACAAGCATTGCCTGAATTAACACCTGATGGA
  GACGTCGTAGCACCAAGTCCAATTTCTTTAAAAAGTTTTGGTCAGAAACAAGAACATAGT
  GCTAGAGAAATGACGAATGAAGAGATTGAACAAGCAATCAAGGATTTTGGTGAAGCAACG
  CGACGTGCAATTGAAGCAGGGTTTGATGGTGTTGAAATACATGGCGCGAATCATTACTTA
  ATTCATCAATTTGTATCACCATACTATAATAGAAGAAATGATGTATGGGCAAATCAATAT
  AAATTCCCGGTCGCTGTGATTGAAGAAGTACTTAAAGCGAAAGAAGCGTATGGCAATAAA
  GACTTTATAGTTGGATACAGATTATCTCCAGAGGAAGCGGAGTCTCCAGGAATCACAATG
  GAAATTACAGAGGAACTCGTTAATAAAATTAGCCATATGCCAATCGACTATATTCATGTT
  TCAATGATGGATACGCATGCAACGACACGTGAAGGTAAATACGCTGGACAAGAAAGACTG
  CCTTTAATTCACAAATGGATAAATGGTCGTATGCCACTTATCGGTATTGGTTCAATTTTC
  ACAGCTGACGAAGCTTTAGATGCAGTTGAAAATGTTGGTGTTGACTTAGTAGCCATTGGT
  AGAGAGCTACTACTGGATTATCAATTTGTTGAAAAAATTAAAGATGGACGGGAAGATGAA
  ATTATTAATTACTTTGATCCAGAGAGAGAAGATAATCATCACTTAACTCCTAATTTATGG
  CATCAATTTAATGAAGGGTTTTATCCATTACCACGTAAAGATAAATAA
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  1128
  

⊟Protein

⊟General

• locus tag: NWMN_0826 [new locus tag: NWMN_RS04665 ]
• symbol: NWMN_0826
• description: NADH-dependent flavin oxidoreductase
• length: 375
• theoretical pI: 5.10559
• theoretical MW: 42109.1
• GRAVY: -0.4712

⊟Function

• TIGRFAM:
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 123.6)
  and 5 more
  mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 69.8)
  Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 21.1)
  dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 14.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other inosine/xanthosine triphosphatase (TIGR00258; HMM-score: 13.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 11.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  TIM_barrel (CL0036) Oxidored_FMN; NADH:flavin oxidoreductase / NADH oxidase family (PF00724; HMM-score: 242.8)
  and 4 more
  TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 17.1)
  DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.8)
  AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 15.1)
  Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010402
  • TAT(Tat/SPI): 0.001511
  • LIPO(Sec/SPII): 0.001339
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221038 NCBI
• RefSeq: YP_001331860 NCBI
• UniProt: A0A0H3KBS1 UniProt

⊟Protein sequence

• MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVELPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSNIEGLKRLATAMKKNGAKALVQIHHGGAQALPELTPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEATRRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYGNKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAGQERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIKDGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications