PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0817 [new locus tag: SA_RS04650 ]
• pan locus tag^?: SAUPAN003058000
• symbol: SA0817
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0817 [new locus tag: SA_RS04650 ]
• symbol: SA0817
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 920455..921582
• length: 1128
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123632 NCBI
• RefSeq: NP_374078 NCBI
• BioCyc: see SA_RS04650
• MicrobesOnline: 103104 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
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  1021
  1081
  
  ATGAAAAGTAAATACGAACCATTGTTTGATAAAGTAGAATTACCAAATGGAGTAGAGTTG
  AGAAATCGATTTGTGTTAGCCCCTTTAACACATATTTCTTCAAATGATGATGGTACTATT
  TCAGATGTAGAACTTCCTTATATTGAAAAGCGTTCACAAGATGTTGGTATTACAATTAAT
  GCTGCGAGTAATGTGAGTGATGTCGGAAAAGCATTTCCAGGACAGCCGTCAATCGCGCAT
  GACAGTGATATTGAAGGACTAAAACGATTAGCTACAGCAATGAAGAAAAACGGTGCCAAA
  GCACTCGTACAAATACATCATGGTGGTGCACAAGCATTGCCTGAATTAATACCTGATGGA
  GACGTCGTAGCACCAAGTCCAATTTCTTTAAAAAGTTTCGGTCAGAAACAAGAACATAGT
  GCTAGAGAAATGACGAATGAAGAGATTGAACAAGCAATCAAGGATTTTGGTGAAGCAACA
  CGACGTGCAATTGAAGCAGGGTTTGATGGTGTTGAAATACATGGCGCGAATCATTACTTA
  ATTCATCAATTTGTATCACCATACTATAATAGAAGAAATGATGTATGGGCAAATCAATAT
  AAATTCCCGGTCGCTGTGATTGAAGAAGTGCTTAAAGCGAAAGAAGCGTATGGCAATAAA
  GACTTTATAGTTGGATACAGATTATCTCCAGAGGAAGCGGAGTCTCCAGGAATCACAATG
  GAAATTACAGAGGAACTCGTTAATAAAATTAGCCATATGCCAATCGACTATATTCATGTT
  TCAATGATGGATACGCATGCAACGACACGTGAAGGTAAATACGCTGGACAAGAAAGACTA
  CCTTTAATTCACAAATGGATAAATGGTCGTATGCCACTTATCGGTATTGGTTCAATTTTC
  ACAGCTGACGAAGCTTTAGATGCAGTTGAAAATGTTGGTGTTGACTTAGTAGCCATTGGT
  AGAGAGCTACTACTGGATTATCAATTTGTTGAAAAAATTAAAGATGGACGGGAAGATGAA
  ATTATTAATTACTTTGATCCAGAGAGAGAAGATAATCATCACTTAACTCCTAATTTATGG
  CATCAATTTAATGAAGGATTCTATCCATTACCACGTAAAGATAAATAA
  60
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  1128
  

⊟Protein

⊟General

• locus tag: SA0817 [new locus tag: SA_RS04650 ]
• symbol: SA0817
• description: hypothetical protein
• length: 375
• theoretical pI: 5.0259
• theoretical MW: 42122.2
• GRAVY: -0.457333

⊟Function

• TIGRFAM:
  mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 121.7)
  and 5 more
  mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 68.9)
  Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 21.1)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other inosine/xanthosine triphosphatase (TIGR00258; HMM-score: 13.7)
  dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 12.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 11.4)
• TheSEED  :
  • NADH-dependent flavin oxidoreductase
• PFAM:
  TIM_barrel (CL0036) Oxidored_FMN; NADH:flavin oxidoreductase / NADH oxidase family (PF00724; HMM-score: 241.8)
  and 4 more
  TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 17)
  DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 16.8)
  AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 14.9)
  Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 13.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010402
  • TAT(Tat/SPI): 0.001511
  • LIPO(Sec/SPII): 0.001339
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926545 NCBI
• RefSeq: NP_374078 NCBI
• UniProt: A0A0H3JM40 UniProt

⊟Protein sequence

• MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVELPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKALVQIHHGGAQALPELIPDGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEATRRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYGNKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAGQERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIKDGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications