PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0844 [new locus tag: NWMN_RS04755 ]
• pan locus tag^?: SAUPAN003111000
• symbol: clpB
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0844 [new locus tag: NWMN_RS04755 ]
• symbol: clpB
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 937403..939217
• length: 1815
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330494 NCBI
• RefSeq: YP_001331878 NCBI
• BioCyc:
• MicrobesOnline: 3706393 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  481
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  1801
  
  ATGAACAAAACAAAGGGTTTTACAAAGTATAAGAAAATGAGATATATTCCAGGGCTCGAT
  GGTTTGAGGGCAATCGCTGTTCTAGGAATTATTATTTACCACTTAAATAAGCAATGGTTG
  ACAGGTGGCTTTTTAGGTGTGGATACATTTTTTGTGATCTCTGGTTATTTAATTACAAGC
  TTATTACTCAAAGAGTATGATGACACAGGTATCATTAAATTGAAAAGCTTTTGGATACGT
  CGTTTAAAACGTTTATTACCAGCAGTCATAGTTTTATTAATGGTTGTAGGGACAGCAACC
  TTATTATTAAAATCAGATAATATCATTAGGGTTAAACATGATATTATTGCTGCGATATTT
  TATGTATCAAACTGGTGGTATATAGCAAAAGATGTTAATTATTTTGAGCAATTTTCATTT
  ATGCCATTAAAGCATTTATGGTCTTTAGCAATTGAAGAACAGTTTTACATATTTTTCCCA
  GTTATTTTGGTTACATTATTGTTAACAATTAAAAAGCGATACAAAATAGGATTTATTTTT
  TGGGGAGTATCAATAATTTCTTTAGGGTTAATGATGTTTATCTATAGTATTAATGGGGAT
  CATTCACGAGTGTATTTTGGTACAGATACTAGATTACAGACATTGTTACTGGGTGTTATT
  TTAGCTTTTTTATGGCCACCGTTTAAATTGAAAAATGATCCACCTAAAGTTGTAAAATAT
  GTTATTGATAGCATAGGTAGTTTATCATTTATAGTACTTATATTATTATTTTTCATTATT
  AATGATGAGACGAATTGGATATATGATGGTGGTTTCTATTTAATATCCATATTAACGTTA
  TTTATTATTGCTAGTGTCGTTCATCCATCTACATGGATAGCGAAGATATTTTCAAATCCA
  GTGTTAGTATTTATCGGGAAAAGGTCTTATAGTTTATATTTATGGCATTTTGCAGTAATT
  AGTTTCGTACATAGTTACTATGTAGACGGACAGATACCTGTATATGTGTACTTTATAGAT
  ATAAGTTTAACAATTATATTTGCAGAGCTATCATATCGCTTTATAGAAACTCCATTTAGA
  AAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATG
  GCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAA
  TATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAAT
  TATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTGAGAAG
  AAAAAGGAATCTTCCGACGTATATAATAATATTAAACCTCTTTTAATCGGTGATTCAGTA
  ATGGTTGATATCGGTGAGTCATTTAAGTCATCAGTTCCTAAGTCTAGAATTGATGGAAAA
  GTAGGGCGTCAATTGTATCAAACCTTACCTTTAGTTAAAGCGAATTATTCACAATATAAA
  AAATCATCTGATCAAGTCGTATTAGAATTAGGTACAAATGGCGACTTTACTGTCAAACAG
  CTCGACGATTTACTTAATCAATTTGGAAAAGCCAAGATTTATTTAGTTAATACACGTGTT
  CCAAGAATTTATGAGGCAAATGTAAATCGATTATTAGCTGACGCGGCGAAACGAAAGTCC
  AATGTCACATTAATTGATTGGTATAAGCGATCACAAGGACATAGTGAATATTTTGCACCA
  GACGGTGTACATTTAGAGTACAAAGGAGTCCTAGCTTTAAAAGATGAAATATTAAAAGCA
  CTTAAAAAGAAATAA
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  1815
  

⊟Protein

⊟General

• locus tag: NWMN_0844 [new locus tag: NWMN_RS04755 ]
• symbol: ClpB
• description: hypothetical protein
• length: 604
• theoretical pI: 9.97192
• theoretical MW: 69444.5
• GRAVY: 0.302815

⊟Function

• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Acyl_transf_3 (CL0316) Acyl_transf_3; Acyltransferase family (PF01757; HMM-score: 83)
  and 1 more
  SGNH_hydrolase (CL0264) Lipase_GDSL_2; GDSL-like Lipase/Acylhydrolase family (PF13472; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008703
  • TAT(Tat/SPI): 0.000122
  • LIPO(Sec/SPII): 0.001113
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151221056 NCBI
• RefSeq: YP_001331878 NCBI
• UniProt: A0A0H3K7S9 UniProt

⊟Protein sequence

• MNKTKGFTKYKKMRYIPGLDGLRAIAVLGIIIYHLNKQWLTGGFLGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLLLKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFPVILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLGVILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLISILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPVYVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFMLILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNNIKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVLELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLIDWYKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications