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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0846 [new locus tag: NWMN_RS04765 ]
  • pan locus tag?: SAUPAN003114000
  • symbol: NWMN_0846
  • pan gene symbol?:
  • synonym:
  • product: LysR family regulatory protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0846 [new locus tag: NWMN_RS04765 ]
  • symbol: NWMN_0846
  • product: LysR family regulatory protein
  • replicon: chromosome
  • strand: -
  • coordinates: 942088..942957
  • length: 870
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAATTTAGATTGGTACTATACATTTGTCATTTTAGCGAAGACGTTAAACTATCGATTA
    GCTAGTGAAGAAATTAATCTCACTATACCTTCTATACATAAGCAAATTAAAAATTTAGAA
    CAGCATCTCAATGTGAAACTATTTGAAACTTATAAAAATCAAATTATCTTGACTGAAGAT
    GGTCATACATTTCTTCCAATTGCGCAAAGTTTTATTGAACAATATGAAAGTGGCATCAAA
    CATATCCAACTTAAAAAAACGATGTTTCAATCGAAATTAAATGTTGTCGTGTCTTCATAT
    ATCGCGACATTCATTATGCCCAAATTTTTAAAATCTTTCTTTAACGAGCATCCTTTTATC
    GATGTTTCTCTTCATGTCAAAAATGAAAATATTGAAAAAGATATTAATAATCATACGTAT
    GACATCGGGATTAGCAGAAATCAACCTAAACTAAGAGAAGTTCATTCTGAAAAAGTATGC
    GAAGGTAAAATTGTATTGATTGCTCCCAATAAAGAGAATAATCATCTTCTAACTGAGGCA
    TCTTTATTTGAAAAATACAAAATAATAAGTGATAACCATCCAGAATATTGGTCATCACTT
    AAAAATAATATTTTAAATATTTATGAAAAAGCACAATTTCTATCTATCAATGATGTGCAT
    ACTTCAATTAAATTAATCGAAATGAATCAAGGTATATCATTCCTGCCTCTTTATATAACA
    ACAAATAGTGATTACAATATTTCGGTTATTAACACGAAAATTTTACAAGCACCTATTTCA
    TTTACATATATATACAGCAAAAAAGAAAGCCCAGAAATATTGGTGTTTATTAAATCATTT
    AAAAAGTATATTGCCAATGAACAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0846 [new locus tag: NWMN_RS04765 ]
  • symbol: NWMN_0846
  • description: LysR family regulatory protein
  • length: 289
  • theoretical pI: 8.35837
  • theoretical MW: 33798.7
  • GRAVY: -0.229412

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions aminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 64.5)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 52.1)
    and 6 more
    Metabolism Energy metabolism Other pca operon transcription factor PcaQ (TIGR02424; HMM-score: 49.5)
    Signal transduction Regulatory functions DNA interactions pca operon transcription factor PcaQ (TIGR02424; HMM-score: 49.5)
    Signal transduction Regulatory functions DNA interactions D-serine deaminase transcriptional activator (TIGR02036; HMM-score: 47.5)
    Cellular processes Cellular processes Toxin production and resistance transcriptional regulator, ArgP family (TIGR03298; HMM-score: 11.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcriptional regulator, ArgP family (TIGR03298; HMM-score: 11.2)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, ArgP family (TIGR03298; HMM-score: 11.2)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 79.5)
    and 2 more
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 43.7)
    SUKH (CL0526) SUKH_6; SMI1-KNR4 cell-wall (PF14568; HMM-score: 11.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014918
    • TAT(Tat/SPI): 0.000286
    • LIPO(Sec/SPII): 0.001636
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLNVKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIATFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVCEGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSINDVHTSIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIYSKKESPEILVFIKSFKKYIANEQL

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]