PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0978 [new locus tag: NWMN_RS05480 ]
• pan locus tag^?: SAUPAN003340000
• symbol: NWMN_0978
• pan gene symbol^?: ftsW
• synonym:
• product: cell division protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0978 [new locus tag: NWMN_RS05480 ]
• symbol: NWMN_0978
• product: cell division protein
• replicon: chromosome
• strand: +
• coordinates: 1083739..1084965
• length: 1227
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330586 NCBI
• RefSeq: YP_001332012 NCBI
• BioCyc:
• MicrobesOnline: 3706529 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAGAATTTTAGAAGTATTTTACGGTATATTGGTAAAACCTCAAAGTTTATTGATTAT
  CCGTTATTAGTTACATATATTGTATTGAGTTTAATTGGATTAGTGATGGTATATAGTGCA
  AGTATGGTTCCAGCAACTAAAGGCACATTGACTGGTGGTATCGATGTTCCAGGAACGTAT
  TTTTACAACCGACAATTAGCATATGTCATAATGAGTTTTATAATTGTATTTTTTATTGCA
  TTTTTAATGAATGTTAAATTACTGAGTAATATTAAAGTGCAAAAAGGTATGATTATAACT
  ATCGTCTCACTATTATTACTGACGTTAGTAATAGGTAAAGATATTAATGGTTCTAAAAGT
  TGGATAAACTTAGGATTTATGAACTTACAGGCATCTGAGTTATTAAAAATTGCAATCATA
  TTATATATCCCATTTATGATCAGTAAAAAAATGCCTAGAGTATTAAGTAAACCAAAATTA
  ATTTTAAGTCCTATTGTATTAGCATTAGGTTGTACGTTTTTAGTTTTCCTACAAAAAGAC
  GTAGGGCAAACATTACTAATATTAATTATTTTAGTTGCGATCATTTTTTATTCAGGAATT
  GGGGTAAACAAAGTCCTAAGATTTGGTATACCAGCAGTGCTAGGATTCTTAGTAGTATTT
  GTCATTGCATTAATGGCTGGTTGGTTACCAAGTTATTTAACTGCCAGATTTAGTACGCTA
  ACAGATCCATTCCAATTCGAATCAGGAACTGGATACCATATTTCCAATTCATTGCTTGCG
  ATAGGTAACGGTGGCGTATTTGGAAAAGGATTAGGAAATAGTGCAATGAAATTGGGCTAT
  TTACCAGAACCACATACAGATTTTATTTTTGCAATTATTTGCGAAGAATTAGGTTTAATC
  GGAGGATTGCTAGTTATTACTTTAGAGTTCTTTATTGTATATCGTGCCTTCCAGTTTGCA
  AATAAAACATCATCATATTTTTATAAACTTGTGTGTGTTGGGATTGCCACATACTTTGGA
  AGTCAAACGTTTGTAAACATTGGCGGTATTTCGGCAACAATTCCATTAACTGGTGTGCCA
  TTGCCATTTATCAGCTTTGGTGGATCATCAATGATTAGTTTAAGTATTGCTATGGGTTTA
  CTTCTGATTGTAGGTAAACAAATCAAAGTAGACCAGCAACGAAAGAAACAACAACAAAAA
  GTTGATATAAGAAGACAATTTAATTAA
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  1227
  

⊟Protein

⊟General

• locus tag: NWMN_0978 [new locus tag: NWMN_RS05480 ]
• symbol: NWMN_0978
• description: cell division protein
• length: 408
• theoretical pI: 10.3601
• theoretical MW: 44881.8
• GRAVY: 0.8125

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan cell division protein FtsW (TIGR02614; HMM-score: 292.7)
  Cellular processes Cellular processes Cell division cell division protein FtsW (TIGR02614; HMM-score: 292.7)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein RodA (TIGR02210; HMM-score: 268.2)
  Cellular processes Cellular processes Cell division rod shape-determining protein RodA (TIGR02210; HMM-score: 268.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein E (TIGR02615; HMM-score: 234.3)
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein E (TIGR02615; HMM-score: 234.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Membrane_trans (CL0142) FTSW_RODA_SPOVE; Cell cycle protein (PF01098; HMM-score: 303.3)
  and 1 more
  no clan defined IncA; IncA protein (PF04156; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.340562
  • TAT(Tat/SPI): 0.003618
  • LIPO(Sec/SPII): 0.013271
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151221190 NCBI
• RefSeq: YP_001332012 NCBI
• UniProt: A0A0H3K7F2 UniProt

⊟Protein sequence

• MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVPATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIVSLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKLILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLVVFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAMKLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVGIATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQQRKKQQQKVDIRRQFN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications