COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0992 [new locus tag: NWMN_RS05565 ]
• pan locus tag^?:
• symbol: int
• pan gene symbol^?: —
• synonym:
• product: integrase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0992 [new locus tag: NWMN_RS05565 ]
• symbol: int
• product: integrase
• replicon: chromosome
• strand: -
• coordinates: 1098898..1100283
• length: 1386
• essential: unknown

⊟Accession numbers

• Gene ID: 5332394 NCBI
• RefSeq: YP_001332026 NCBI
• BioCyc:
• MicrobesOnline: 3706543 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGAAAGTAGCAATTTATACTAGAGTGAGTACACTTGAACAAAAAGAAAAAGGACACTCT
  ATCGAAGAACAAGAAAGAAAATTAAGAGCTTACAGCGACATAAACGACTGGAAAATTCAT
  AAAGTATATACTGACGCTGGATACTCCGGAGCTAAAAAAGACAGACCCGCTTTACAAGAA
  ATGTTGAATGAAATAGATAATTTTGATTTGGTTTTAGTCTATAAACTAGATCGATTAACT
  CGAAGTGTTAAAGACTTACTAGAGATACTAGAATTGTTTGAGAATAAAAACGTGTTGTTT
  AGGAGCGCAACAGAAGTATATGACACAACTTCTGCTATGGGACGTTTGTTCGTAACATTA
  GTAGGTGCTATGGCAGAGTGGGAGCGTACTACAATTCAAGAGCGTACTGCAATGGGTCGA
  CGCGCATCAGCTAGAAAAGGGTTAGCTAAAACTGTCCCTCCTTTCTATTACGACAGAGTA
  AACGATAAATTTGTGCCTAATGAATATAAAAAAGTATTACGATTTGCAGTAGAAGAAGCG
  AAAAAAGGTACTAGTTTAAGAGAAATAACTATAAAATTGAACAACTCTAAATACAAAGCA
  CCCTTAGGTAAAAACTGGCACAGATCAGTTATAGGCAATGCTCTAACGAGTCCGGTAGCT
  AGAGGTCATCTTGTTTTCGGTGACATATTCGTCGAAAACACCCACGAAGCTATTATAAGT
  GAAGAAGAATACGAAGAAATAAAATTAAGGATAAGTGAAAAAACTAACTCTACAATCGTA
  AAACATAACGCTATTTTCAGAAGTAAACTATTATGTCCAAACTGTAACCAGAAATTGACT
  TTAAACACAGTCAAGCATACGCCTAAAAATAAAGAAGTTTGGTATTCTAAACTATACTTT
  TGTTCTAACTGCAAAAATACTAAAAATAAAAATGCATGTAACATCGACGAAGGCGAGGTT
  TTAAAACAATTTTACAATTATCTAAAACAATTTGATTTAACATCATATAAAATCGAAAAC
  CAACCTAAAGAAATAGAAGATGTCGGCATCGATATTGAAAAGTTGCGAAAAGAACGCGCT
  AGATGTCAAACACTTTTTATAGAAGGTATGATGGATAAGGATGAAGCTTTTCCAATAATA
  AGTCGTATTGACAAAGAAATACATGAGTATGAAAAGCGCAAGGATAATGATAAGGGTAAG
  ACTTTTAACTATGAGAAGATTAAAAATTTCAAGTATTCATTGCTAAACGGCTGGGAATTA
  ATGGAAGATGAGTTAAAAACTGAATTCATAAAGATGGCAATCAAAAACATTCATTTTGAA
  TATGTAAAAGGAATTAAAGGGAAGCGCCAGAACTCATTGAAGATTACGGGTATAGAGTTT
  TATTAA
  60
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  840
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  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1386
  

⊟Protein

⊟General

• locus tag: NWMN_0992 [new locus tag: NWMN_RS05565 ]
• symbol: Int
• description: integrase
• length: 461
• theoretical pI: 9.54826
• theoretical MW: 53970.6
• GRAVY: -0.660087

⊟Function

• TIGRFAM:
• TheSEED:
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 146.5)
  and 7 more
  Recombinase; Recombinase (PF07508; HMM-score: 49)
  Zn_Beta_Ribbon (CL0167) Zn_ribbon_recom; Recombinase zinc beta ribbon domain (PF13408; HMM-score: 18.3)
  RNase_H (CL0219) DDE_Tnp_1_6; Transposase DDE domain (PF13751; HMM-score: 15.4)
  Zn_Beta_Ribbon (CL0167) Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 14.4)
  DZR; Double zinc ribbon (PF12773; HMM-score: 11.5)
  zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 9.5)
  zf-GRF; GRF zinc finger (PF06839; HMM-score: 9.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009895
  • TAT(Tat/SPI): 0.000856
  • LIPO(Sec/SPII): 0.002188
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221204 NCBI
• RefSeq: YP_001332026 NCBI
• UniProt: A0A0H3KFR8 UniProt

⊟Protein sequence

• MKVAIYTRVSTLEQKEKGHSIEEQERKLRAYSDINDWKIHKVYTDAGYSGAKKDRPALQEMLNEIDNFDLVLVYKLDRLTRSVKDLLEILELFENKNVLFRSATEVYDTTSAMGRLFVTLVGAMAEWERTTIQERTAMGRRASARKGLAKTVPPFYYDRVNDKFVPNEYKKVLRFAVEEAKKGTSLREITIKLNNSKYKAPLGKNWHRSVIGNALTSPVARGHLVFGDIFVENTHEAIISEEEYEEIKLRISEKTNSTIVKHNAIFRSKLLCPNCNQKLTLNTVKHTPKNKEVWYSKLYFCSNCKNTKNKNACNIDEGEVLKQFYNYLKQFDLTSYKIENQPKEIEDVGIDIEKLRKERARCQTLFIEGMMDKDEAFPIISRIDKEIHEYEKRKDNDKGKTFNYEKIKNFKYSLLNGWELMEDELKTEFIKMAIKNIHFEYVKGIKGKRQNSLKITGIEFY

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications