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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1038 [new locus tag: NWMN_RS05875 ]
  • pan locus tag?: SAUPAN001705000
  • symbol: NWMN_1038
  • pan gene symbol?:
  • synonym:
  • product: phage holin

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1038 [new locus tag: NWMN_RS05875 ]
  • symbol: NWMN_1038
  • product: phage holin
  • replicon: chromosome
  • strand: +
  • coordinates: 1139484..1139921
  • length: 438
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGATATTAACTGGAAATTGAGATTTAAAAATAAAGCAGTATTAACAGGTTTAGTTGGT
    GCATTGTTGTTATTTATCAAACAAATCACAGATTTATTCGGATTCGATTTATCAACTCAA
    TTAAATCAAGCCAGCGCGATTATAGGTGCTATCCTCACGCTACTTACAGGGATTGGCGTT
    ATTACTGACCCAACGTCAAAAGGTGTTGCTGATTCATCAATAGCACAGACATATCAAGCG
    CCTAGAGATAGTAGCAAAGAAGAACAACAAGTCACTTGGAAAACTTCACAAGATACTAGC
    TTAACACCGGAATTAAGTACAAAAGCACCGAAAGAGTACGATACATCACAACCATTTACA
    GACACCTCTAATGAAATCGGTTTTGACGTGAACGAGTATCATCACGGAGGTGGCGACAAT
    GCAAGCAAAATTGACTAA
    60
    120
    180
    240
    300
    360
    420
    438

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1038 [new locus tag: NWMN_RS05875 ]
  • symbol: NWMN_1038
  • description: phage holin
  • length: 145
  • theoretical pI: 4.52831
  • theoretical MW: 15753.5
  • GRAVY: -0.310345

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions holin, phage phi LC3 family (TIGR01598; HMM-score: 116.2)
  • TheSEED:
  • PFAM:
    no clan defined Phage_holin_1; Bacteriophage holin (PF04531; HMM-score: 93)
    and 2 more
    RP1-2; Tubuliform egg casing silk strands structural domain (PF12042; HMM-score: 15.3)
    DUF4727; Domain of unknown function (DUF4727) (PF15856; HMM-score: 12.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.055484
    • TAT(Tat/SPI): 0.000593
    • LIPO(Sec/SPII): 0.004262
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDINWKLRFKNKAVLTGLVGALLLFIKQITDLFGFDLSTQLNQASAIIGAILTLLTGIGVITDPTSKGVADSSIAQTYQAPRDSSKEEQQVTWKTSQDTSLTPELSTKAPKEYDTSQPFTDTSNEIGFDVNEYHHGGGDNASKID

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]