PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1039 [new locus tag: NWMN_RS05880 ]
• pan locus tag^?: SAUPAN001706000
• symbol: NWMN_1039
• pan gene symbol^?: —
• synonym:
• product: phage amidase

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1039 [new locus tag: NWMN_RS05880 ]
• symbol: NWMN_1039
• product: phage amidase
• replicon: chromosome
• strand: +
• coordinates: 1139902..1141347
• length: 1446
• essential: unknown

⊟Accession numbers

• Gene ID: 5330643 NCBI
• RefSeq: YP_001332073 NCBI
• BioCyc:
• MicrobesOnline: 3706590 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGCAAGCAAAATTGACTAAAAAAGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAA
  CAATTTAATATCGACCTTTGGTATGCATTTCAATGCTTTGATTATGCTAATGCTGGTTGG
  AAAGCTTTGTTTGGATTACTCCTAAAAGGTGTAGGCGCAAAAGATATTCCGTTCGCTAAC
  AACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGC
  GACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATT
  GAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACT
  GACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTAT
  GATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTT
  CAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTAAGCCACAACCTAAAGCAGTAGAA
  CTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCT
  AAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGT
  AACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTA
  TCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAAT
  CAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAAC
  GCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGTGCTAGGTTATTAAAAAAG
  TGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCA
  TGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTG
  CCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATG
  GATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTT
  AAACCAGTTGCGAGTGCATGGAAACGTAATAAATATGGTACTTACTACATGGAAGAAAGT
  GCTAGATTCACAAACGGCAATCAACCAATCACAGTAAGAAAAGTGGGGCCATTCTTATCT
  TGTCCAGTGGGTTATCAGTTCCAACCTGGTGGATATTGTGATTATACAGAAGTGATGTTA
  CAAGATGGTCATGTTTGGGTAGGATATACATGGGAGGGGCAACGTTATTACTTGCCTATT
  AGAACATGGAATGGTTCTTCCCCACCTAATCAGATATTAGGTGACTTATGGGGAGAAATC
  AGTTAG
  60
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  1320
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  1440
  1446
  

⊟Protein

⊟General

• locus tag: NWMN_1039 [new locus tag: NWMN_RS05880 ]
• symbol: NWMN_1039
• description: phage amidase
• length: 481
• theoretical pI: 8.78887
• theoretical MW: 54045.7
• GRAVY: -0.471518

⊟Function

• TIGRFAM:
• TheSEED:
• PFAM:
  SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 85.8)
  and 3 more
  no clan defined Amidase_2; N-acetylmuramoyl-L-alanine amidase (PF01510; HMM-score: 57.8)
  Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 43.4)
  HTH (CL0123) Myb_DNA-bind_2; Rap1 Myb domain (PF08914; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.1
  • Cytoplasmic Membrane Score: 0.14
  • Cellwall Score: 0.15
  • Extracellular Score: 9.6
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.027647
  • TAT(Tat/SPI): 0.000585
  • LIPO(Sec/SPII): 0.000799
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221251 NCBI
• RefSeq: YP_001332073 NCBI
• UniProt: A0A0H3K875 UniProt

⊟Protein sequence

• MQAKLTKKEFIEWLKTSEGKQFNIDLWYAFQCFDYANAGWKALFGLLLKGVGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSSPPNQILGDLWGEIS

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1038 > NWMN_1039

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.