PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1116 [new locus tag: NWMN_RS06305 ]
• pan locus tag^?: SAUPAN003485000
• symbol: pyrE
• pan gene symbol^?: pyrE
• synonym:
• product: orotate phosphoribosyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1116 [new locus tag: NWMN_RS06305 ]
• symbol: pyrE
• product: orotate phosphoribosyltransferase
• replicon: chromosome
• strand: +
• coordinates: 1222882..1223493
• length: 612
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332293 NCBI
• RefSeq: YP_001332150 NCBI
• BioCyc:
• MicrobesOnline: 3706668 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGCTAAAGAAATTGCAAAATCATTATTAGATATTGAAGCTGTAACATTATCACCAAAT
  GATTTATATACATGGAGTTCAGGTATTAAATCACCGATTTACTGTGATAACCGTGTTACG
  TTAGGTTATCCTTTAGTTCGAGGCGCAATCCGCGATGGTTTAATTAACTTAATTAAAGAA
  CACTTTCCTGAAGTAGAAGTTATTTCTGGTACTGCAACAGCTGGTATTCCACATGCAGCT
  TTTATTGCTGAAAAATTAAAATTACCAATGAATTATGTTCGTTCATCAAATAAGAGTCAT
  GGTAAGCAAAATCAAATCGAAGGTGCTAAAAGTGAAGGTAAAAAAGTAGTTGTGATAGAA
  GATTTAATTTCGACAGGGGGATCTTCAGTCACAGCAGTTGAAGCCTTAAAACTAGCAGGT
  GCAGAAGTATTAGGTGTTGTAGCTATCTTTACTTACGGTTTGAAAAAAGCAGATGATACA
  TTTAGCAATATTCAACTACCTTTTTACACTTTAAGTGATTACAATGAATTAATTGAAGTA
  GCTGAAAATGAAGGTAAAATTTCTAGTGAAGATATCCAAACATTAGTTGAATGGAGAGAC
  AACTTAGCATAA
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  612
  

⊟Protein

⊟General

• locus tag: NWMN_1116 [new locus tag: NWMN_RS06305 ]
• symbol: PyrE
• description: orotate phosphoribosyltransferase
• length: 203
• theoretical pI: 4.79327
• theoretical MW: 22042
• GRAVY: -0.0325123

⊟Function

• reaction:
  EC 2.4.2.10?  ExPASy
  Orotate phosphoribosyltransferase Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 149.1)
  and 9 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 59.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 37.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 31)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides xanthine phosphoribosyltransferase (TIGR01744; EC 2.4.2.22; HMM-score: 20.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis pur operon repressor PurR (TIGR01743; HMM-score: 19.2)
  Signal transduction Regulatory functions DNA interactions pur operon repressor PurR (TIGR01743; HMM-score: 19.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides uracil phosphoribosyltransferase (TIGR01091; EC 2.4.2.9; HMM-score: 16.5)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 16.4)
  Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 14.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 64.7)
  and 2 more
  UPRTase; Uracil phosphoribosyltransferase (PF14681; HMM-score: 14.8)
  Leu-IlvD (CL0364) Aconitase_2_N; Aconitate hydratase 2 N-terminus (PF06434; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008509
  • TAT(Tat/SPI): 0.000197
  • LIPO(Sec/SPII): 0.000517
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221328 NCBI
• RefSeq: YP_001332150 NCBI
• UniProt: A0A0H3KCC6 UniProt

⊟Protein sequence

• MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYPLVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGKQNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDTFSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: pyrP > pyrB > pyrC > pyrAA > carB > pryF > pyrE > NWMN_1117

⊟Regulation

• regulator: pyrR leader (transcription antitermination) regulon

  pyrR leader

  (RNA)

  important in Pyrimidine metabolism; transcription unit transferred from N315 data RegPrecise binding of the PryR antitermination protein

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications