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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
  • pan locus tag?: SAUPAN003626000
  • symbol: glnA
  • pan gene symbol?: glnA
  • synonym:
  • product: glutamine synthetase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
  • symbol: glnA
  • product: glutamine synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1342749..1344104
  • length: 1356
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGGAGGATTTTAAAATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCA
    GAAGAGGAAAATGTAAGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAA
    AATGTTGAAGTGCCTGTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGAC
    GGTTCTTCTATCGAAGGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGAT
    TTAGATACTTGGGTAATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATT
    TGTGATGTATATAAAACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAA
    CGTGTATTAAAAGAAATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCA
    GAATTCTTCTTGTTTAAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGAT
    GGTGGATATTTCGATTTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTT
    TTAGAATTAGAGGATATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGT
    CAACATGAAATTGACTTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACA
    TTTAAATTGGTTGTTAAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCT
    AAACCATTATTCGGTGTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGT
    AAAGAAAATGCATTCTTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAA
    TTTACAGCAGGTGTGCTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTA
    AACTCATATAAACGTTTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGT
    AAAAACCGTTCACCATTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAA
    GTACGTTCAGTAGATCCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCT
    GGACTAGATGGTATTAAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTAC
    GAAATGAACCGTGAAGAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTAT
    ACTGCATTAAAAGCAATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATC
    TATAATCAATTTATTAATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTCTCT
    GAATGGGAAAGAGATCAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1356

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
  • symbol: GlnA
  • description: glutamine synthetase
  • length: 451
  • theoretical pI: 4.80101
  • theoretical MW: 51491.1
  • GRAVY: -0.447007

Function[edit | edit source]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamine synthetase ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 581.7)
    and 1 more
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
    and 2 more
    no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
    Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002452
    • TAT(Tat/SPI): 0.000194
    • LIPO(Sec/SPII): 0.00026
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEDFKMPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulators: CodY (repression) regulon, GlnR (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    GlnR(TF)important in Nitrogen assimilation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]