PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
• pan locus tag^?: SAUPAN003626000
• symbol: glnA
• pan gene symbol^?: glnA
• synonym:
• product: glutamine synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
• symbol: glnA
• product: glutamine synthetase
• replicon: chromosome
• strand: +
• coordinates: 1342749..1344104
• length: 1356
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331956 NCBI
• RefSeq: YP_001332251 NCBI
• BioCyc:
• MicrobesOnline: 3706769 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  TTGGAGGATTTTAAAATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCA
  GAAGAGGAAAATGTAAGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAA
  AATGTTGAAGTGCCTGTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGAC
  GGTTCTTCTATCGAAGGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGAT
  TTAGATACTTGGGTAATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATT
  TGTGATGTATATAAAACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAA
  CGTGTATTAAAAGAAATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCA
  GAATTCTTCTTGTTTAAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGAT
  GGTGGATATTTCGATTTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTT
  TTAGAATTAGAGGATATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGT
  CAACATGAAATTGACTTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACA
  TTTAAATTGGTTGTTAAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCT
  AAACCATTATTCGGTGTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGT
  AAAGAAAATGCATTCTTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAA
  TTTACAGCAGGTGTGCTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTA
  AACTCATATAAACGTTTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGT
  AAAAACCGTTCACCATTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAA
  GTACGTTCAGTAGATCCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCT
  GGACTAGATGGTATTAAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTAC
  GAAATGAACCGTGAAGAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTAT
  ACTGCATTAAAAGCAATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATC
  TATAATCAATTTATTAATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTCTCT
  GAATGGGAAAGAGATCAGTACATGAAGCAATATTAA
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⊟Protein

⊟General

• locus tag: NWMN_1217 [new locus tag: NWMN_RS06840 ]
• symbol: GlnA
• description: glutamine synthetase
• length: 451
• theoretical pI: 4.80101
• theoretical MW: 51491.1
• GRAVY: -0.447007

⊟Function

• reaction:
  EC 6.3.1.2?  ExPASy
  Glutamine synthetase ATP + L-glutamate + NH₃ = ADP + phosphate + L-glutamine
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 581.7)
  and 1 more
  glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
  and 2 more
  no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
  Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002452
  • TAT(Tat/SPI): 0.000194
  • LIPO(Sec/SPII): 0.00026
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221429 NCBI
• RefSeq: YP_001332251 NCBI
• UniProt: A0A0H3K7V5 UniProt

⊟Protein sequence

• MEDFKMPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: glnR > glnA

⊟Regulation

• regulators: CodY (repression) regulon, GlnR (repression) regulon

  CodY	(TF)	important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise
  GlnR	(TF)	important in Nitrogen assimilation; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications