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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1329 [new locus tag: SACOL_RS06770 ]
  • pan locus tag?: SAUPAN003626000
  • symbol: femC
  • pan gene symbol?: glnA
  • synonym:
  • product: glutamine synthetase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1329 [new locus tag: SACOL_RS06770 ]
  • symbol: femC
  • product: glutamine synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1346582..1347922
  • length: 1341
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCAGAAGAGGAAAATGTA
    AGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCT
    GTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGACGGTTCTTCTATCGAA
    GGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGATTTAGATACTTGGGTA
    ATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATTTGTGATGTATATAAA
    ACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAACGTGTATTAAAAGAA
    ATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCAGAATTCTTCTTGTTT
    AAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
    TTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGAT
    ATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGTCAACATGAAATTGAC
    TTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACATTTAAATTGGTTGTT
    AAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAACCATTATTCGGT
    GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTC
    TTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAATTTACAGCAGGTGTG
    CTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTAAACTCATATAAACGT
    TTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGTAAAAACCGTTCACCA
    TTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAAGTACGTTCAGTAGAT
    CCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCTGGACTAGATGGTATT
    AAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTACGAAATGAACCGTGAA
    GAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTATACTGCATTAAAAGCA
    ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATCTATAATCAATTTATT
    AATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTCTCTGAATGGGAAAGAGAT
    CAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL1329 [new locus tag: SACOL_RS06770 ]
  • symbol: FemC
  • description: glutamine synthetase
  • length: 446
  • theoretical pI: 4.83865
  • theoretical MW: 50840.4
  • GRAVY: -0.438117

Function[edit | edit source]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamine synthetase ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 581.8)
    and 1 more
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
  • TheSEED:  
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine synthetases  Glutamine synthetase type I (EC 6.3.1.2)
    and 4 more
    Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  Glutamine synthetase type I (EC 6.3.1.2)
    PII Superfamily  Glutamine synthetase type I (EC 6.3.1.2)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Glutamine synthetase type I (EC 6.3.1.2)
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Ammonia assimilation  Glutamine synthetase type I (EC 6.3.1.2)
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
    and 2 more
    no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
    Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.3)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001979
    • TAT(Tat/SPI): 0.000395
    • LIPO(Sec/SPII): 0.000266
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3] [4]
    quantitative data / protein copy number per cell: 8398 [5]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: CodY (repression) regulon, GlnR (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise  
    GlnR(TF)important in Nitrogen assimilation; RegPrecise    transcription unit transferred from N315 data RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: 20.95 h [6]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]