PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1240 [new locus tag: NWMN_RS07000 ]
• pan locus tag^?: SAUPAN003700000
• symbol: metL
• pan gene symbol^?: hom
• synonym:
• product: homoserine dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1240 [new locus tag: NWMN_RS07000 ]
• symbol: metL
• product: homoserine dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 1363375..1364655
• length: 1281
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332539 NCBI
• RefSeq: YP_001332274 NCBI
• BioCyc:
• MicrobesOnline: 3706792 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAATTAAATATAGCATTATTAGGATTAGGTACTGTCGGATCTGGTGTTGTTAAA
  ATCATCGAAGAGAACCGACAGCAAATTCAAGATACATTAAATAAAGATATTGTCATAAAG
  CATATTCTTGTTCGAGATAAATCTAAAAAGAGACCGCTAAATATTAGCCAATATCATTTA
  ACTGAAGATGTTAATGAAATTTTAAATGATGATTCATTAGATATTATCGTTGAAGTCATG
  GGAGGAATTGAACCAACTGTAGATTGGTTAAGAACAGCACTTAAAAATAAAAAACATGTT
  ATTACCGCAAATAAAGATTTATTAGCAGTACATCTTAAACTTTTAGAAGATTTAGCAGAA
  GAAAATGGTGTAGCTTTAAAGTTTGAAGCGAGTGTAGCAGGTGGTATTCCGATCGTAAAT
  GCCATAAATAATGGTTTGAATGCGAATAATATTTCAAAATTTATGGGAATTTTAAATGGT
  ACCTCTAATTTTATTTTATCTAAAATGACTAAAGAGCAAACGACATTTGAGGAAGCACTT
  GATGAAGCGAAAAGACTTGGTTTTGCTGAAGCGGATCCAACTGATGATGTAGAAGGGGTA
  GATGCAGCGCGTAAAGTTGTCATTACATCATATTTATCATTTAACCAAGTCATTAAATTA
  AACGACGTTAAACGAAGAGGAATTAGTGGCGTAACTTTAACTGATATTAATGTAGCCGAT
  CAACTGGGGTATAAAATTAAATTGATTGGTAAGGGAATATATGAAAATGGCAAAGTTAAT
  GCATCGGTAGAACCAACGTTAATTGATAAAAAGCATCAATTAGCAGCTGTAGAGGATGAA
  TATAACGCGATTTATGTTATTGGTGATGCCGTTGGTGACACGATGTTTTATGGAAAAGGA
  GCAGGCAGTTTAGCAACAGGTAGTGCCGTTGTCAGTGATTTATTGAATGTAGCATTATTC
  TTTGAATCAGATTTACACACATTGCCACCACATTTTGAATTAAAGACAGATAAAACACGG
  GAAATGATGGATTCAGATGCAGAAATTAATATTAAAGAAAAATCCAATTTCTTTGTAGTA
  GTGAATCATGTCAAAGGTTCAATTGAAAATTTTGAAAATGAGTTAAAGGCAATATTACCA
  TTTCACCGATCATTAAGAGTTGCAAATTACGATAATCAATCATATGCCGCTGTTATAGTT
  GGATTGGAATCATCACCGGAAGAATTAATCACTAAGCATGGATACGAAGTTGACAAAGTA
  TACCCAGTAGAAGGAGTTTAA
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⊟Protein

⊟General

• locus tag: NWMN_1240 [new locus tag: NWMN_RS07000 ]
• symbol: MetL
• description: homoserine dehydrogenase
• length: 426
• theoretical pI: 5.04475
• theoretical MW: 46873.2
• GRAVY: -0.126291

⊟Function

• reaction:
  EC 1.1.1.3?  ExPASy
  Homoserine dehydrogenase L-homoserine + NAD(P)⁺ = L-aspartate 4-semialdehyde + NAD(P)H
• TIGRFAM:
  Signal transduction Regulatory functions Protein interactions Phr family secreted Rap phosphatase inhibitor (TIGR04429; HMM-score: 12.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined Homoserine_dh; Homoserine dehydrogenase (PF00742; HMM-score: 201.5)
  and 5 more
  NADP_Rossmann (CL0063) NAD_binding_3; Homoserine dehydrogenase, NAD binding domain (PF03447; HMM-score: 65.9)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 14)
  DXP_reductoisom; 1-deoxy-D-xylulose 5-phosphate reductoisomerase (PF02670; HMM-score: 13.6)
  no clan defined Img2; Mitochondrial large subunit ribosomal protein (Img2) (PF05046; HMM-score: 13.3)
  HAD (CL0137) Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.144791
  • TAT(Tat/SPI): 0.000632
  • LIPO(Sec/SPII): 0.040296
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221452 NCBI
• RefSeq: YP_001332274 NCBI
• UniProt: A0A0H3K8E1 UniProt

⊟Protein sequence

• MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNGTSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVIKLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFELKTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDNQSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: metL > thrC > thrB > NWMN_1243

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications