PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1243 [new locus tag: NWMN_RS07015 ]
• pan locus tag^?: SAUPAN003703000
• symbol: NWMN_1243
• pan gene symbol^?: —
• synonym:
• product: haloacid dehalogenase-like hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1243 [new locus tag: NWMN_RS07015 ]
• symbol: NWMN_1243
• product: haloacid dehalogenase-like hydrolase
• replicon: chromosome
• strand: +
• coordinates: 1366696..1367499
• length: 804
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332540 NCBI
• RefSeq: YP_001332277 NCBI
• BioCyc:
• MicrobesOnline: 3706795 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAAATTATAAAGTTGTCGTTTTAGATATGGATGACACATTGCTAAATTCAGATAAT
  GTGATATCAGAAGAAACTGCAAATTATTTAACAGCAATTCAAGATGAAGGTTATTATGTT
  GTTCTAGCATCTGGTAGACCTACTGAAGGTATGATTCCAACTGCTAGAGATTTAAAATTA
  CCTGAACATCATAGCTATATTATTAGTTATAACGGTAGTAAAACGATTAACATGACTAAT
  GAAGAAGTAGAAGTAAGTAAATCGATTGGTAAGCAAGATTTCGATGAAATTGTAGATTAT
  TGTCGAGATAGAGGCTTTTTCGTTCTTACATATCATGATGGTCAAATTATTTACGACAGC
  GAACATGAGTATATGAATATTGAAGCAGAATTAACAGGTTTACCGATGAAACGTGTTGAT
  GATATCAAAGCGTATATTCAAGGCGATGTACCCAAGGTCATGGGTGTAGATTATGTAGCG
  AATATTACAGAAGCTAGAATTGATTTGAATGGTGTGTTCAATGATAATGTAGATGCTACG
  ACAAGTAAGCCATTCTTCTTAGAATTTATGGCCAAAGACGTTTCAAAAGGTAATGCAATT
  AAAGCGTTATGTCACAAATTGGGATATTCGGTGGATCAAGTCATTGCTTTTGGTGATAGT
  ATGAATGATAAATCAATGTTTGAAGTCGCAGGTCTAGCTATTGCTATGGGGAATGCATCA
  GATGAACTTAAGCAATATGCAAATGAAGTTACGTTGGATCATAATGAAAATGGTATTCCA
  CATGCGCTCAAAAAATTGTTATAA
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⊟Protein

⊟General

• locus tag: NWMN_1243 [new locus tag: NWMN_RS07015 ]
• symbol: NWMN_1243
• description: haloacid dehalogenase-like hydrolase
• length: 267
• theoretical pI: 4.32652
• theoretical MW: 29821.4
• GRAVY: -0.271536

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 186.2)
  and 23 more
  phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 86.9)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 84.9)
  sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 81.5)
  mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 50.4)
  sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 34.9)
  Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 33.1)
  phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 33)
  sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 32.8)
  mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 32.3)
  Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 30.8)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 26.2)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 23.9)
  Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 22.9)
  Metabolism Energy metabolism Anaerobic phenylphosphate carboxylase, delta subunit (TIGR02726; HMM-score: 22.6)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 22.3)
  hemimethylated DNA binding domain (TIGR02097; HMM-score: 16.6)
  HAD phosphatase, family IIIC (TIGR01681; HMM-score: 16)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 15.4)
  heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 14.7)
  HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 14.6)
  Cellular processes Cellular processes Adaptations to atypical conditions trehalose-phosphatase (TIGR00685; EC 3.1.3.12; HMM-score: 12.8)
  Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 11.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 11.5)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 219.5)
  and 9 more
  S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 57)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 27.8)
  HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 24.4)
  Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 19.8)
  DUF2608; Protein of unknown function (DUF2608) (PF11019; HMM-score: 18.3)
  HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 17.2)
  no clan defined YccV-like; Hemimethylated DNA-binding protein YccV like (PF08755; HMM-score: 15.9)
  HAD (CL0137) Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 14.5)
  no clan defined TMEM240; TMEM240 family (PF15207; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005564
  • TAT(Tat/SPI): 0.000279
  • LIPO(Sec/SPII): 0.000792
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221455 NCBI
• RefSeq: YP_001332277 NCBI
• UniProt: A0A0H3K7X2 UniProt

⊟Protein sequence

• MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLASGRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCRDRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVANITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFGDSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: metL > thrC > thrB > NWMN_1243

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications