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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1297 [new locus tag: NWMN_RS07305 ]
- pan locus tag?: SAUPAN003794000
- symbol: pstB
- pan gene symbol?: pstB
- synonym:
- product: phosphate transporter ATP-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1297 [new locus tag: NWMN_RS07305 ]
- symbol: pstB
- product: phosphate transporter ATP-binding protein
- replicon: chromosome
- strand: -
- coordinates: 1428384..1429235
- length: 852
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5332273 NCBI
- RefSeq: YP_001332331 NCBI
- BioCyc:
- MicrobesOnline: 3706849 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGGCGCAAACACTTGCACAAACTAAACAAATATCTCAAAGTCATACGTTTGATGTCTCA
CAAAGTCATCATAAAACACCAGATGATACAAACTCACATTCTGTTATATATTCAACACAA
AATTTAGACTTATGGTATGGCGAAAATCATGCATTACAAAATATTAATTTAGATATTTAT
GAAAACCAAATTACTGCCATTATAGGTCCATCTGGTTGTGGCAAATCAACATATATTAAA
ACTTTGAATCGAATGGTTGAGTTAGTACCTTCTGTGAAAACAGCTGGTAAAATATTATAT
CGAGATCAAGACATTTTTGATCAAAAATATTCTAAAGAACAATTACGTACAAATGTGGGC
ATGGTCTTTCAACAACCTAATCCATTTCCAAAATCAATATACGATAATATTACTTACGGT
CCAAAGATTCACGGTATTAAAAATAAAAAAGTTCTTGATGAAATCGTTGAGAAATCATTA
CGTGGCGCTGCAATTTGGGATGAATTAAAGGATAGGTTGCACACAAATGCATATAGTTTA
TCCGGTGGGCAACAACAACGTGTTTGTATCGCGCGTTGTTTAGCAATTGAACCTGAAGTC
ATTTTAATGGATGAACCGACATCAGCATTAGATCCAATCTCAACATTAAGAGTAGAAGAG
TTGGTTCAAGAACTAAAAGAAAAGTATACAATTATTATGGTTACACATAATATGCAACAA
GCAGCTCGTGTATCAGATAAAACTGCATTTTTCTTAAATGGTTATGTCAATGAATATGAT
GATACTGATAAAATTTTCTCTAACCCATCAAACAAGAAAACAGAAGATTATATTTCAGGA
AGGTTTGGTTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1297 [new locus tag: NWMN_RS07305 ]
- symbol: PstB
- description: phosphate transporter ATP-binding protein
- length: 283
- theoretical pI: 6.51418
- theoretical MW: 32154.1
- GRAVY: -0.537809
⊟Function[edit | edit source]
- reaction: EC 3.6.3.27? ExPASyPhosphate-transporting ATPase ATP + H2O + phosphate(Out) = ADP + phosphate + phosphate(In)
- TIGRFAM: Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 427)and 77 moreTransport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 172.3)Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 166.2)Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 150)ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 141.2)Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 136.1)Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 134.2)D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 126.6)thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 126.2)Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 125.1)Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 123.5)2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 118.9)Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 118.8)Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 116.7)Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 116.5)Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 115.5)Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 114.3)Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 114.3)Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 114.2)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 114.2)Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 112.9)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 110.3)Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 110.3)Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 109.1)Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 109)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 108.5)Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 108.5)Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 108.3)ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 106.7)ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 103.9)thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 102.3)Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 101.9)Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 100.9)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 100.1)Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 98.9)Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 98.9)Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 98.5)Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 96.7)Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 96.7)Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 96.3)gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 89.1)Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 88.9)Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 88.9)lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 86.6)Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 85)Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 85)Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 79.6)Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 79.2)Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 77.9)proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 75.6)Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 74.7)Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 69.6)phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 68.5)Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 67.6)Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 64.9)Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 59.7)Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 59.7)Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 59.2)ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 59.1)Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 57.8)Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 57.6)Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 57.6)Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 47.2)Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 43.9)DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 41.5)Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 39.7)Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 39.5)Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 39.5)Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 28.4)Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 27.2)DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 27.2)DNA metabolism DNA replication, recombination, and repair checkpoint protein rad24 (TIGR00602; HMM-score: 21.4)Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 14.4)Central intermediary metabolism Phosphorus compounds phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 13.9)Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 12.9)Ti-type conjugative transfer relaxase TraA (TIGR02768; HMM-score: 10.5)
- TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
- PFAM: P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 117.3)and 27 moreSMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 39.4)AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 37.9)AAA_15; AAA ATPase domain (PF13175; HMM-score: 24.7)AAA_22; AAA domain (PF13401; HMM-score: 21.3)Rad17; Rad17 cell cycle checkpoint protein (PF03215; HMM-score: 20.6)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 20.5)AAA_23; AAA domain (PF13476; HMM-score: 19.6)AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 19.2)RNA_helicase; RNA helicase (PF00910; HMM-score: 17.1)AAA_10; AAA-like domain (PF12846; HMM-score: 17.1)PRK; Phosphoribulokinase / Uridine kinase family (PF00485; HMM-score: 15.7)AAA_18; AAA domain (PF13238; HMM-score: 15.7)AAA_30; AAA domain (PF13604; HMM-score: 15.7)AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.3)AAA_14; AAA domain (PF13173; HMM-score: 14.8)AAA_28; AAA domain (PF13521; HMM-score: 14.5)AAA_24; AAA domain (PF13479; HMM-score: 14.3)Viral_helicase1; Viral (Superfamily 1) RNA helicase (PF01443; HMM-score: 14.2)G-alpha; G-protein alpha subunit (PF00503; HMM-score: 13.9)AAA_19; AAA domain (PF13245; HMM-score: 13.7)PhoH; PhoH-like protein (PF02562; HMM-score: 13.4)AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13.1)ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.8)Adeno_IVa2; Adenovirus IVa2 protein (PF02456; HMM-score: 12.7)ABC_ATPase; Predicted ATPase of the ABC class (PF09818; HMM-score: 12.6)no clan defined VF530; DNA-binding protein VF530 (PF09905; HMM-score: 12.5)P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 12.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010288
- TAT(Tat/SPI): 0.000684
- LIPO(Sec/SPII): 0.001655
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDLWYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRDQDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSLRGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRVEELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTEDYISGRFG
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: phoU < pstB < NWMN_1298 < NWMN_1299
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)