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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1375 [new locus tag: NWMN_RS07740 ]
  • pan locus tag?: SAUPAN003924000
  • symbol: aroA
  • pan gene symbol?: aroA
  • synonym:
  • product: 3-phosphoshikimate 1-carboxyvinyltransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1375 [new locus tag: NWMN_RS07740 ]
  • symbol: aroA
  • product: 3-phosphoshikimate 1-carboxyvinyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1540857..1542155
  • length: 1299
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGTAAATGAACAAATCATTGATATTTCAGGTCCGTTAAAGGGCGAAATAGAAGTGCCG
    GGCGATAAGTCAATGACACACCGTGCAATCATGTTGGCGTCGCTAGCTGAAGGTGTATCT
    ACTATATATAAGCCACTACTTGGCGAAGATTGTCGTCGTACGATGGACATTTTCCGACTG
    TTAGGTGTAGAAATCAAAGAAGATGATGAAAAATTAGTTGTGACTTCCCCAGGATATCAA
    TCTTTTAACACGCCACATCAAGTATTGTATACAGGTAATTCTGGTACGACAACACGATTA
    TTGGCAGGTTTGTTAAGTGGTTTAGGTATTGAAAGTGTTTTGTCTGGCGATGTTTCAATT
    GGTAAAAGGCCAATGGATCGTGTCTTGAGACCATTGAAACTTATGGATGCGAATATTGAA
    GGTATTGAAGATAATTATACACCATTAATTATTAAGCCATCTGTCATAAAAGGTATAAAT
    TATCAAATGGAAGTTGCAAGTGCACAAGTAAAAAGTGCCATTTTATTTGCAAGTTTGTTT
    TCTAAGGAACCGACCATCATTAAAGAATTAGATGTAAGTCGAAATCATACTGAGACGATG
    TTCAAACATTTTAATATTCCAATTGAAGCAGAAGGGTTATCAATTAATACAACCCCTGAA
    GCAATTCGATACATTAAACCTGCAGATTTTCATGTTCCTGGCGATATTTCATCTGCAGCG
    TTCTTTATTGTTGCAGCACTTATCACACCAGGAAGTGATGTAACAATTCATAATGTTGGA
    ATCAATCCAACACGTTCAGGTATTATTGATATTGTTGAAAAAATGGGCGGTAATATCCAA
    CTTTTCAATCAAACAACTGGTGCTGAACCTACTGCTTCTATTCGTATTCAATACACACCA
    ATGCTTCAACCAATAACAATCGAAGGAGAATTAGTTCCAAAAGCAATTGATGAACTGCCT
    GTAATAGCATTACTTTGTACACAAGCAGTTGGCACGAGTACAATTAAAGATGCCGAGGAA
    TTAAAAGTAAAAGAAACAAATAGAATTGATACAACGGCTGATATGTTAAACTTGTTAGGG
    TTTGAATTACAACCAACTAATGATGGATTGATTATTCATCCGTCAGAATTTAAAACAAAT
    GCAACAGTTGATAGTTTAACTGATCATCGAATAGGAATGATGCTTGCAGTTGCTTCTCTA
    CTTTCAAGCGAGCCTGTCAAAATCAAACAATTTGATGCTGTAAATGTATCATTTCCAGGA
    TTTTTACCAAAACTAAAGCTTTTAGAAAATGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1299

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1375 [new locus tag: NWMN_RS07740 ]
  • symbol: AroA
  • description: 3-phosphoshikimate 1-carboxyvinyltransferase
  • length: 432
  • theoretical pI: 4.77676
  • theoretical MW: 47002.9
  • GRAVY: 0.0409722

Function[edit | edit source]

  • reaction:
    EC 2.5.1.19?  ExPASy
    3-phosphoshikimate 1-carboxyvinyltransferase Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Aromatic amino acid family 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 379.1)
    and 1 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 72.7)
  • TheSEED:  
    Amino Acids and Derivatives Aromatic amino acids and derivatives Chorismate Synthesis  5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)
    and 3 more
    Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate)  5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)
    5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) alpha sub-homology division
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Tryp Chlamydiae  5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)
  • PFAM:
    EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 455.3)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004546
    • TAT(Tat/SPI): 0.001018
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]