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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
  • pan locus tag?: SAUPAN004076000
  • symbol: ispA
  • pan gene symbol?: ispA
  • synonym:
  • product: geranyltranstransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
  • symbol: ispA
  • product: geranyltranstransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1598865..1599746
  • length: 882
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACGAATCTACCGATGAATAAATTAATAGATGAAGTCAATAATGAATTATCGGTTGCG
    ATAAATAAATCAGTAATGGATACTCAGCTAGAAGAAAGTATGTTGTATTCATTAAATGCT
    GGAGGTAAACGCATCCGACCAGTTCTGTTATTACTCACTTTAGATTCACTAAATACCGAG
    TATGAGTTAGGTATGAAGAGCGCAATTGCACTAGAAATGATTCATACATATTCACTTATT
    CATGATGACCTACCAGCGATGGATAATGATGATTATCGACGAGGAAAATTAACAAATCAT
    AAAGTATATGGTGAGTGGACTGCGATATTAGCAGGTGATGCTTTATTAACTAAAGCATTT
    GAACTTATTTCAAGTGATGATAGATTAACTGATGAAGTAAAAATAAAAGTTCTACAACGG
    CTGTCAATAGCAAGTGGTCATGTTGGAATGGTCGGCGGTCAAATGTTAGATATGCAAAGC
    GAAGGCCAACCAATTGATCTTGAAACTTTGGAAATGATACACAAAACAAAAACAGGAGCA
    TTATTAACTTTTGCGGTTATGAGTGCAGCAGATATCGCTAATGTCGATGATACAACTAAA
    GAACATTTAGAAAGTTATAGTTATCATTTAGGTATGATGTTCCAGATTAAAGATGATTTA
    TTAGACTGCTATGGTGATGAAGCAAAGTTAGGTAAAAAAGTGGGCAGCGATCTTGAAAAT
    AATAAAAGTACGTACGTGAGTTTATTAGGGAAAGATGGCGCAGAAGATAAATTGACTTAT
    CATAGAGACGCAGCAGTGGATGAACTAACGCAAATTGATGAACAATTCAATACAAAACAC
    TTATTAGAAATCGTTGATTTATTTTATAGCAGGGATCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
  • symbol: IspA
  • description: geranyltranstransferase
  • length: 293
  • theoretical pI: 4.49116
  • theoretical MW: 32808.1
  • GRAVY: -0.272696

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 119.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 104.5)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 188.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002222
    • TAT(Tat/SPI): 0.000218
    • LIPO(Sec/SPII): 0.00037
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKRIRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKVYGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQSEGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKDDLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQFNTKHLLEIVDLFYSRDH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • ispA no polycistronic organisation predicted

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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