NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
• pan locus tag^?: SAUPAN004076000
• symbol: ispA
• pan gene symbol^?: ispA
• synonym:
• product: geranyltranstransferase

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
• symbol: ispA
• product: geranyltranstransferase
• replicon: chromosome
• strand: -
• coordinates: 1598865..1599746
• length: 882
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331725 NCBI
• RefSeq: YP_001332461 NCBI
• BioCyc:
• MicrobesOnline: 3706979 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGACGAATCTACCGATGAATAAATTAATAGATGAAGTCAATAATGAATTATCGGTTGCG
  ATAAATAAATCAGTAATGGATACTCAGCTAGAAGAAAGTATGTTGTATTCATTAAATGCT
  GGAGGTAAACGCATCCGACCAGTTCTGTTATTACTCACTTTAGATTCACTAAATACCGAG
  TATGAGTTAGGTATGAAGAGCGCAATTGCACTAGAAATGATTCATACATATTCACTTATT
  CATGATGACCTACCAGCGATGGATAATGATGATTATCGACGAGGAAAATTAACAAATCAT
  AAAGTATATGGTGAGTGGACTGCGATATTAGCAGGTGATGCTTTATTAACTAAAGCATTT
  GAACTTATTTCAAGTGATGATAGATTAACTGATGAAGTAAAAATAAAAGTTCTACAACGG
  CTGTCAATAGCAAGTGGTCATGTTGGAATGGTCGGCGGTCAAATGTTAGATATGCAAAGC
  GAAGGCCAACCAATTGATCTTGAAACTTTGGAAATGATACACAAAACAAAAACAGGAGCA
  TTATTAACTTTTGCGGTTATGAGTGCAGCAGATATCGCTAATGTCGATGATACAACTAAA
  GAACATTTAGAAAGTTATAGTTATCATTTAGGTATGATGTTCCAGATTAAAGATGATTTA
  TTAGACTGCTATGGTGATGAAGCAAAGTTAGGTAAAAAAGTGGGCAGCGATCTTGAAAAT
  AATAAAAGTACGTACGTGAGTTTATTAGGGAAAGATGGCGCAGAAGATAAATTGACTTAT
  CATAGAGACGCAGCAGTGGATGAACTAACGCAAATTGATGAACAATTCAATACAAAACAC
  TTATTAGAAATCGTTGATTTATTTTATAGCAGGGATCACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  882
  

⊟Protein

⊟General

• locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
• symbol: IspA
• description: geranyltranstransferase
• length: 293
• theoretical pI: 4.49116
• theoretical MW: 32808.1
• GRAVY: -0.272696

⊟Function

• TIGRFAM:
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneheptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 119.1)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonesolanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 104.5)
• TheSEED:  
  Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsArchaeal lipids (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  and 15 more
  Dimethylallyltransferase (EC 2.5.1.1)
  Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)
  archaeal lipids bobik (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Carotenoids (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)
  Isoprenoid Biosynthesis (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Dimethylallyltransferase (EC 2.5.1.1)
  Isoprenoid Biosynthesis: Interconversions (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Isoprenoinds for Quinones (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Dimethylallyltransferase (EC 2.5.1.1)
  Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)
  Octaprenyl diphosphate synthase (EC 2.5.1.90)
  Polyprenyl Diphosphate Biosynthesis (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)
  Octaprenyl diphosphate synthase (EC 2.5.1.90)
• PFAM:
  Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 188.6)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002222
  • TAT(Tat/SPI): 0.000218
  • LIPO(Sec/SPII): 0.00037
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221639 NCBI
• RefSeq: YP_001332461 NCBI
• UniProt: A0A0H3K8P7 UniProt

⊟Protein sequence

• MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKRIRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKVYGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQSEGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKDDLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQFNTKHLLEIVDLFYSRDH

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• ispA no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.