PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
• pan locus tag^?: SAUPAN004076000
• symbol: ispA
• pan gene symbol^?: ispA
• synonym:
• product: geranyltranstransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
• symbol: ispA
• product: geranyltranstransferase
• replicon: chromosome
• strand: -
• coordinates: 1598865..1599746
• length: 882
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331725 NCBI
• RefSeq: YP_001332461 NCBI
• BioCyc:
• MicrobesOnline: 3706979 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACGAATCTACCGATGAATAAATTAATAGATGAAGTCAATAATGAATTATCGGTTGCG
  ATAAATAAATCAGTAATGGATACTCAGCTAGAAGAAAGTATGTTGTATTCATTAAATGCT
  GGAGGTAAACGCATCCGACCAGTTCTGTTATTACTCACTTTAGATTCACTAAATACCGAG
  TATGAGTTAGGTATGAAGAGCGCAATTGCACTAGAAATGATTCATACATATTCACTTATT
  CATGATGACCTACCAGCGATGGATAATGATGATTATCGACGAGGAAAATTAACAAATCAT
  AAAGTATATGGTGAGTGGACTGCGATATTAGCAGGTGATGCTTTATTAACTAAAGCATTT
  GAACTTATTTCAAGTGATGATAGATTAACTGATGAAGTAAAAATAAAAGTTCTACAACGG
  CTGTCAATAGCAAGTGGTCATGTTGGAATGGTCGGCGGTCAAATGTTAGATATGCAAAGC
  GAAGGCCAACCAATTGATCTTGAAACTTTGGAAATGATACACAAAACAAAAACAGGAGCA
  TTATTAACTTTTGCGGTTATGAGTGCAGCAGATATCGCTAATGTCGATGATACAACTAAA
  GAACATTTAGAAAGTTATAGTTATCATTTAGGTATGATGTTCCAGATTAAAGATGATTTA
  TTAGACTGCTATGGTGATGAAGCAAAGTTAGGTAAAAAAGTGGGCAGCGATCTTGAAAAT
  AATAAAAGTACGTACGTGAGTTTATTAGGGAAAGATGGCGCAGAAGATAAATTGACTTAT
  CATAGAGACGCAGCAGTGGATGAACTAACGCAAATTGATGAACAATTCAATACAAAACAC
  TTATTAGAAATCGTTGATTTATTTTATAGCAGGGATCACTAA
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⊟Protein

⊟General

• locus tag: NWMN_1427 [new locus tag: NWMN_RS08055 ]
• symbol: IspA
• description: geranyltranstransferase
• length: 293
• theoretical pI: 4.49116
• theoretical MW: 32808.1
• GRAVY: -0.272696

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 119.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 104.5)
• TheSEED  :
  • (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  • Dimethylallyltransferase (EC 2.5.1.1)
  • Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)
  • Octaprenyl diphosphate synthase (EC 2.5.1.90)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids  (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  and 5 more
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Carotenoids  (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis  (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis: Interconversions  (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoinds for Quinones  (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
  Fatty Acids, Lipids, and Isoprenoids Isoprenoids Polyprenyl Diphosphate Biosynthesis  (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
• PFAM:
  Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 191.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9914
  • Cytoplasmic Membrane Score: 0.0068
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0018
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002222
  • TAT(Tat/SPI): 0.000218
  • LIPO(Sec/SPII): 0.00037
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221639 NCBI
• RefSeq: YP_001332461 NCBI
• UniProt: A0A0H3K8P7 UniProt

⊟Protein sequence

• MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKRIRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKVYGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQSEGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKDDLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQFNTKHLLEIVDLFYSRDH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications