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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1566 [new locus tag: SACOL_RS07975 ]
- pan locus tag?: SAUPAN004076000
- symbol: ispA
- pan gene symbol?: ispA
- synonym:
- product: geranyltranstransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1566 [new locus tag: SACOL_RS07975 ]
- symbol: ispA
- product: geranyltranstransferase
- replicon: chromosome
- strand: -
- coordinates: 1602685..1603566
- length: 882
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237242 NCBI
- RefSeq: YP_186407 NCBI
- BioCyc:
- MicrobesOnline: 913015 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGACGAATCTACCGATGAATAAATTAATAGATGAAGTCAATAATGAATTATCGGTTGCG
ATAAATAAATCAGTAATGGATACTCAGCTAGAAGAAAGTATGTTGTATTCATTAAATGCT
GGAGGTAAACGCATCCGACCAGTTCTGTTATTACTCACTTTAGATTCACTAAATACCGAG
TATGAGTTAGGTATGAAGAGCGCAATTGCACTAGAAATGATTCATACATATTCACTTATT
CATGATGACCTACCAGCGATGGATAATGATGATTATCGACGAGGAAAATTAACAAATCAT
AAAGTATATGGTGAGTGGACTGCGATATTAGCAGGTGATGCTTTATTAACTAAAGCATTT
GAACTTATTTCAAGTGATGATAGATTAACTGATGAAGTAAAAATAAAAGTTCTACAACGG
CTGTCAATAGCAAGTGGTCATGTTGGAATGGTCGGCGGTCAAATGTTAGATATGCAAAGC
GAAGGCCAACCAATTGATCTTGAAACTTTGGAAATGATACACAAAACAAAAACAGGAGCA
TTATTAACTTTTGCGGTTATGAGTGCAGCAGATATCGCTAATGTCGATGATACAACTAAA
GAACATTTAGAAAGTTATAGTTATCATTTAGGTATGATGTTCCAGATTAAAGATGATTTA
TTAGACTGCTATGGTGATGAAGCAAAGTTAGGTAAAAAAGTGGGCAGCGATCTTGAAAAT
AATAAAAGTACGTACGTGAGTTTATTAGGGAAAGATGGCGCAGAAGATAAATTGACTTAT
CATAGAGACGCAGCAGTGGATGAACTAACGCAAATTGATGAACAATTCAATACAAAACAC
TTATTAGAAATCGTTGATTTATTTTATAGCAGGGATCACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1566 [new locus tag: SACOL_RS07975 ]
- symbol: IspA
- description: geranyltranstransferase
- length: 293
- theoretical pI: 4.49116
- theoretical MW: 32808.1
- GRAVY: -0.272696
⊟Function[edit | edit source]
- reaction: EC 2.5.1.10? ExPASy(2E,6E)-farnesyl diphosphate synthase Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 119.1)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 104.5)
- TheSEED: Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)and 15 moreDimethylallyltransferase (EC 2.5.1.1)Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)Dimethylallyltransferase (EC 2.5.1.1)Dimethylallyltransferase (EC 2.5.1.1)Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)Octaprenyl diphosphate synthase (EC 2.5.1.90)Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)Octaprenyl diphosphate synthase (EC 2.5.1.90)
- PFAM: Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 188.6)
⊟Structure, modifications & interactions[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002222
- TAT(Tat/SPI): 0.000218
- LIPO(Sec/SPII): 0.00037
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKRIRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKVYGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQSEGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKDDLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQFNTKHLLEIVDLFYSRDH
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlasquantitative data / protein copy number per cell: 199 [4]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: 22.66 h [5]
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑
Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑
Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑
Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑
Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑
Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]