PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1591
• pan locus tag^?: SAUPAN004323000
• symbol: NWMN_1591
• pan gene symbol^?: —
• synonym:
• product: putative transposase for IS1272

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1591
• symbol: NWMN_1591
• product: putative transposase for IS1272
• replicon: chromosome
• strand: -
• coordinates: 1763298..1764125
• length: 828
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332549 NCBI
• RefSeq: YP_001332625 NCBI
• BioCyc:
• MicrobesOnline: 3707143 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTATAAAATTTATAACATGACCCAACTTACACTACCAATAGAAACCTCTGTTAGAATT
  CCTCAAAATGATATTTCGCGATATGTTAATGAAATTGTTGAAACGATACCTGATAGCGAA
  TTCGATGAATTCAGACATCATCGTGGCGCAACATCCTATCATCCAAAAATGATGTTAAAA
  ATCATCTTATATGCATATACTCAATCTGTATTTTCTGGTCGTAGAATAGAAAAATTACTT
  CATGACAGTATTCGAATGATGTGGTTAGCTCAAGATCAAACACCTTCTTATAAAACTATT
  AATCGTTTTAGAGTGAATCCTAATACTGATGCGTTAATTGAATCTTTATTTATTCAGTTC
  CATAGTCAATGTTTAAAGCAAAATCTTATTGATAATAATTCAATTTTTATTGATGGTACA
  AAAGTAGAAGCTAATGCCAATAGATATACATTTGTGTGGAAGAAAAGTATTCAAAATCAC
  GAATCGAAATTGAACGAAAATTCAAAAACATTATATCGTGACTTAGTTGAAGAAAAAATA
  ATACCAGAGATAAAAGAAGATGGAGATAGCGATTTAACAATAGAAGAAATAGATTTAATT
  GGTAGTCATTTAGATAAAGAAATCGAAGATTTAAATCATTCTATTGAGAACGAAGATTGT
  GCTCAAATTAGAAAACAGACCCGTAAAAAAATAACTGAGATTAAGAAGTTCAAAAAGAAA
  TTTGATGATTATTCCGAAAGAAAAAATAAATATGAAGAACAAAAATCGATTCTTAAAGAT
  AGAAATAGTTTTTCTAAAACTGATCTGATCATGATGCAACTTTTATGA
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⊟Protein

⊟General

• locus tag: NWMN_1591
• symbol: NWMN_1591
• description: putative transposase for IS1272
• length: 275
• theoretical pI: 6.96526
• theoretical MW: 32667
• GRAVY: -0.736

⊟Function

• TIGRFAM:
• TheSEED: data available for NCTC8325
• PFAM:
  no clan defined DUF772; Transposase domain (DUF772) (PF05598; HMM-score: 59.9)
  and 4 more
  DUF2139; Uncharacterized protein conserved in archaea (DUF2139) (PF09910; HMM-score: 11.5)
  DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 10.7)
  PCRF; PCRF domain (PF03462; HMM-score: 9.3)
  NTP_transf (CL0260) PolyA_pol; Poly A polymerase head domain (PF01743; HMM-score: 7.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012412
  • TAT(Tat/SPI): 0.000185
  • LIPO(Sec/SPII): 0.001221
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221803 NCBI
• RefSeq: YP_001332625 NCBI
• UniProt: A0A0H3KH03 UniProt

⊟Protein sequence

• MYKIYNMTQLTLPIETSVRIPQNDISRYVNEIVETIPDSEFDEFRHHRGATSYHPKMMLKIILYAYTQSVFSGRRIEKLLHDSIRMMWLAQDQTPSYKTINRFRVNPNTDALIESLFIQFHSQCLKQNLIDNNSIFIDGTKVEANANRYTFVWKKSIQNHESKLNENSKTLYRDLVEEKIIPEIKEDGDSDLTIEEIDLIGSHLDKEIEDLNHSIENEDCAQIRKQTRKKITEIKKFKKKFDDYSERKNKYEEQKSILKDRNSFSKTDLIMMQLL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: tnp < NWMN_1591

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications