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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1594 [new locus tag: NWMN_RS08940 ]
  • pan locus tag?: SAUPAN004347000
  • symbol: accA
  • pan gene symbol?: accA
  • synonym:
  • product: acetyl-CoA carboxylase carboxyltransferase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1594 [new locus tag: NWMN_RS08940 ]
  • symbol: accA
  • product: acetyl-CoA carboxylase carboxyltransferase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1767782..1768726
  • length: 945
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAGATTTTGAAAAACCACTTTTTGAAATTCGAAATAAAATTGAATCTTTAAAAGAA
    TCTCAAGATAAAAATGATGTGGATTTACAAGAAGAAATTGACATGCTTGAAGCGTCATTG
    GAACGAGAAACTAAAAAAATATATACAAATCTAAAACCATGGGATCGTGTGCAAATTGCG
    CGTTTGCAAGAAAGACCTACGACCCTAGATTATATTCCATATATCTTTGATTCGTTTATG
    GAACTACATGGTGATCGTAATTTTAGAGATGATCCAGCAATGATTGGTGGTATTGGCTTT
    TTAAATGGTCGTGCTGTTACAGTTATTGGACAACAACGTGGAAAAGATACAAAAGATAAT
    ATTTATCGAAATTTTGGTATGGCGCATCCAGAAGGTTATCGAAAAGCATTACGTTTAATG
    AAACAAGCTGAAAAATTCAATCGTCCTATCTTTACATTTATAGATACAAAAGGTGCATAT
    CCTGGTAAAGCTGCTGAAGAACGTGGACAAAGTGAATCTATCGCAACAAATTTGATTGAG
    ATGGCTTCATTAAAAGTACCAGTTATTGCGATTGTCATTGGTGAAGGTGGCAGTGGAGGT
    GCTCTAGGTATTGGTATTGCCAATAAAGTATTGATGTTAGAGAATAGTACTTACTCTGTT
    ATATCTCCTGAAGGTGCAGCGGCATTATTATGGAAAGACAGTAATTTGGCTAAAATTGCA
    GCTGAAACAATGAAAATTACTGCCCATGATATTAAGCAATTAGGTATTATAGATGATGTC
    ATTTCTGAACCACTTGGCGGTGCACATAAAGATATTGAACAGCAAGCTTTAGCTATTAAA
    TCAGCGTTTGTTGCACAGTTAGATTCACTTGAGTCATTATCACGTGATGAAATTGCTAAT
    GATCGCTTTGAAAAATTCAGAAATATCGGTTCTTATATAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

⊟Protein[edit | edit source]

Protein Data Bank: 2F9I
Protein Data Bank: 5KDR

⊟General[edit | edit source]

  • locus tag: NWMN_1594 [new locus tag: NWMN_RS08940 ]
  • symbol: AccA
  • description: acetyl-CoA carboxylase carboxyltransferase subunit alpha
  • length: 314
  • theoretical pI: 4.95687
  • theoretical MW: 35069.7
  • GRAVY: -0.340764

⊟Function[edit | edit source]

  • reaction:
    EC 6.4.1.2?  ExPASy
    Acetyl-CoA carboxylase ATP + acetyl-CoA + HCO3(-) = ADP + phosphate + malonyl-CoA
  • TIGRFAM:
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, carboxyl transferase, alpha subunit (TIGR00513; EC 6.4.1.2; HMM-score: 486.1)
    and 4 more
    biotin-independent malonate decarboxylase, gamma subunit (TIGR03134; EC 4.1.1.88; HMM-score: 45.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
    Metabolism Energy metabolism Fermentation methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
    Metabolism Energy metabolism ATP-proton motive force interconversion methylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    ClpP_crotonase (CL0127) ACCA; Acetyl co-enzyme A carboxylase carboxyltransferase alpha subunit (PF03255; HMM-score: 211.4)
    and 3 more
    Carboxyl_trans; Carboxyl transferase domain (PF01039; HMM-score: 55.1)
    MdcE; Malonate decarboxylase gamma subunit (MdcE) (PF06833; HMM-score: 18.7)
    ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 17)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001877
    • TAT(Tat/SPI): 0.000609
    • LIPO(Sec/SPII): 0.000422
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERETKKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLNGRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAYPGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTYSVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQALAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_1595(accD)acetyl-CoA carboxylase subunit beta  [1] (data from MRSA252)
    NWMN_1605(ackA)acetate kinase  [1] (data from MRSA252)
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_0745(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    NWMN_1096(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0741(gapA)glyceraldehyde 3-phosphate dehydrogenase 1  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_2040(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0960(phdB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2152(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    NWMN_2151(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_1613(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_2127(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)
    NWMN_1604universal stress protein family protein  [1] (data from MRSA252)
    NWMN_2504malate:quinone oxidoreductase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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