PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1660 [new locus tag: NWMN_RS09320 ]
• pan locus tag^?: SAUPAN004456000
• symbol: ribA
• pan gene symbol^?: ribA
• synonym:
• product: riboflavin biosynthesis protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1660 [new locus tag: NWMN_RS09320 ]
• symbol: ribA
• product: riboflavin biosynthesis protein
• replicon: chromosome
• strand: -
• coordinates: 1855927..1857108
• length: 1182
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332355 NCBI
• RefSeq: YP_001332694 NCBI
• BioCyc:
• MicrobesOnline: 3707212 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCAATTCGATAATATTGACAGTGCTTTAATGGCTTTAAAAAATGGAGAACCAATTATT
  GTAGTAGATGATGAGAATCGTGAAAATGAAGGTGATTTAGTAGCGGTTACTGAATGGATG
  AACGATAATACCATTAATTTTATGGCGAAAGAAGCAAGGGGATTAATATGCGCACCAGTG
  TCTAAAGATATTGCACAACGTTTGGATTTGGTACAAATGGTTGATGATAACTCCGACATC
  TTTGGTACGCAATTTACAGTGAGTATTGATCATGTAGATACAACAACAGGAATTAGTGCT
  TATGAACGTACATTGACTGCCAAAAAGCTCATTGATCCTAGTAGTGAAGCTAAAGATTTT
  AATCGTCCTGGTCATTTATTTCCATTAGTAGCACAAGATAAAGGCGTATTAGCTAGAAAT
  GGACACACAGAAGCGGCTGTTGATTTAGCTAAACTTACTGGTGCCAAGCCCGCTGGTGTC
  ATTTGTGAGATTATGAATGATGACGGCACGATGGCGAAAGGACAAGATTTACAAAAGTTT
  AAAGAAAAACATCAATTAAAGATGATTACGATTGATGATTTAATTGAATATCGTAAAAAA
  TTAGAACCAGAAATTGAATTTAAGGCAAAAGTGAAAATGCCTACAGATTTCGGAACATTT
  GATATGTATGGTTTTAAAGCGACATACACAGATGAAGAGATAGTTGTACTGACAAAAGGT
  GCAATTCGACAACATGAAAATGTACGCTTACATTCTGCGTGCCTTACAGGCGATATTTTC
  CATAGTCAACGTTGTGATTGTGGTGCTCAACTTGAATCGTCTATGAAGTATATCAATGAA
  CATGGTGGCATGATTATTTATCTACCTCAAGAAGGTCGTGGCATAGGATTGTTAAACAAA
  TTACGCGCATATGAATTAATTGAGCAAGGATATGATACAGTAACTGCAAATTTAGCATTA
  GGTTTTGATGAAGATTTGCGAGATTATCATATTGCTGCACAGATTTTAAAATATTTTAAC
  ATCGAACATATCAATTTATTAAGTAATAATCCAAGTAAATTTGAGGGATTAAAACAATAT
  GGCATTGATATTGCAGAAAGAATTGAAGTTATCGTACCAGAAACGGTACATAATCATGAT
  TATATGGAAACGAAAAAAATAAAAATGGGTCATTTAATATAG
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⊟Protein

⊟General

• locus tag: NWMN_1660 [new locus tag: NWMN_RS09320 ]
• symbol: RibA
• description: riboflavin biosynthesis protein
• length: 393
• theoretical pI: 4.88903
• theoretical MW: 44188.1
• GRAVY: -0.326718

⊟Function

• reaction:
  EC 3.5.4.25?  ExPASy
  GTP cyclohydrolase II GTP + 3 H₂O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
  EC 4.1.99.12?  ExPASy
  3,4-dihydroxy-2-butanone-4-phosphate synthase D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD 3,4-dihydroxy-2-butanone-4-phosphate synthase (TIGR00506; EC 4.1.99.12; HMM-score: 247.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD GTP cyclohydrolase II (TIGR00505; EC 3.5.4.25; HMM-score: 227)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined DHBP_synthase; 3,4-dihydroxy-2-butanone 4-phosphate synthase (PF00926; HMM-score: 268)
  and 1 more
  GTP_cyclohydro2; GTP cyclohydrolase II (PF00925; HMM-score: 209.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺, Mn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002296
  • TAT(Tat/SPI): 0.000204
  • LIPO(Sec/SPII): 0.000515
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221872 NCBI
• RefSeq: YP_001332694 NCBI
• UniProt: A0A0H3K947 UniProt

⊟Protein sequence

• MQFDNIDSALMALKNGEPIIVVDDENRENEGDLVAVTEWMNDNTINFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYERTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGVICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFGTFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMKYINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHLI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ribH < ribA < ribB < ribD

⊟Regulation

• regulator: FMN-box (transcription termination) regulon

  FMN-box

  (RNA)

  important in Riboflavin biosynthesis; transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications