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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1734 [new locus tag: NWMN_RS09770 ]
  • pan locus tag?: SAUPAN004772000
  • symbol: NWMN_1734
  • pan gene symbol?: cbf1
  • synonym:
  • product: 3'-5' exoribonuclease YhaM

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1734 [new locus tag: NWMN_RS09770 ]
  • symbol: NWMN_1734
  • product: 3'-5' exoribonuclease YhaM
  • replicon: chromosome
  • strand: -
  • coordinates: 1933181..1934122
  • length: 942
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGAAATATAGAGAATCTAAATCCCGGAGATTCAGTTGATCACTTTTTCTTAGTGCAT
    AAAGCTACACAGGGTGTAACAGCACAAGGTAAAGATTATATGACATTACATTTGCAAGAT
    AAAAGTGGTGAAATTGAAGCGAAATTTTGGACGGCTACAAAAAATGATATGGCAACAATC
    AAGCCTGAAGAAATTGTACATGTTAAAGGTGACATCATAAACTATCGCGGAAATAAACAG
    ATGAAAGTCAACCAAATTAGACTAGCGACAACTGAAGATCAATTAAAAACAGAACAATTT
    GTAGATGGTGCACCTTTATCACCGGCAGAAATACAAGAAGAGATTTCTCATTATTTGCTA
    GATATTGAAAATGCTAATTTACAACGTATCACACGTCATTTATTGAAAAAATATCAAGAA
    CGATTTTACACATATCCAGCTGCTAGTTCTCATCATCATAACTTTGCGAGTGGCTTAAGC
    TATCATGTATTAACGATGTTACGTATTGCAAAATCAATTTGTGACATTTATCCATTGTTA
    AACAAAAGTTTGTTATATAGTGGTATTATTTTGCATGATATTGGTAAAGTTAGAGAATTG
    AGTGGTCCTGTTGCGACGTCGTATACAGTCGAAGGTAACTTATTAGGACACATCTCGATT
    GCGAGTGATGAAGTAGTTGAAGCAGCTCGTGAATTGAACATTGAAGGAGAAGAAATCATG
    TTGTTAAGACATATGATTTTATCTCATCATGGTAAGTTAGAGTATGGTTCTCCAAAACTG
    CCATACTTAAAAGAAGCAGAAATTTTATGCTATATCGATAATATCGATGCTAGAATGAAT
    ATGTTTGAAAAGGCATATAAAAAAACTGACAAGGGTCAGTTTACAGATAAAATATTTGGT
    CTTGAAAATCGTAGATTCTACAATCCTGAATCACTCGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    942

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1734 [new locus tag: NWMN_RS09770 ]
  • symbol: NWMN_1734
  • description: 3'-5' exoribonuclease YhaM
  • length: 313
  • theoretical pI: 6.56818
  • theoretical MW: 35778.6
  • GRAVY: -0.417891

⊟Function[edit | edit source]

  • TIGRFAM:
    CRISPR-associated endonuclease Cas3-HD (TIGR01596; EC 3.1.-.-; HMM-score: 23.3)
    HDIG domain (TIGR00277; HMM-score: 20.9)
    and 1 more
    integrating conjugative element relaxase, PFGI-1 class (TIGR03760; HMM-score: 13.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 41.2)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 34.9)
    and 1 more
    HD_PDEase (CL0237) TraI_2; Putative helicase (PF07514; HMM-score: 18.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013949
    • TAT(Tat/SPI): 0.001698
    • LIPO(Sec/SPII): 0.001301
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGEIEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVDGAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLSYHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHISIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNIDARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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