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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1836 [new locus tag: NWMN_RS10550 ]
  • pan locus tag?: SAUPAN004922000
  • symbol: NWMN_1836
  • pan gene symbol?: dgkB
  • synonym:
  • product: putative lipid kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1836 [new locus tag: NWMN_RS10550 ]
  • symbol: NWMN_1836
  • product: putative lipid kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2042893..2043840
  • length: 948
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGGAAACGTGCTAGAATCATTTATAACCCGACATCAGGTAAAGAGCTATTTAAAAGA
    GAATTACCTGATGCCTTAATAAAATTAGAAAAAGCGGGATATGAAACGAGTGCATATGCA
    ACCGAGAAAATAGGTGATGCCACACTTGAAGCAGAAAGAGCTATGCATGAAAATTATGAT
    GTATTAATCGCTGCAGGTGGTGATGGAACATTAAATGAAGTAGTTAATGGTATCGCAGAA
    AAGCCTAATCGTCCTAAGCTAGGTGTCATTCCTATGGGTACTGTTAATGACTTTGGACGT
    GCATTGCATATACCTAATGACATCATGGGGGCACTTGATGTCATCATTGAAGGTCATTCT
    ACTAAAGTAGATATTGGTAAAATGAATAATCGATACTTTATTAATTTAGCTGCAGGCGGA
    CAATTGACGCAAGTCTCTTATGAAACACCGAGTAAATTGAAATCTATTGTTGGTCCATTT
    GCTTATTACATCAAAGGTTTCGAAATGTTACCTCAAATGAAAGCTGTAGATTTAAGAATT
    GAATATGATGGTAATGTTTTCCAAGGAGAAGCATTATTATTCTTTTTAGGTTTAACAAAT
    TCAATGGCAGGATTTGAAAAATTAGTGCCGGACGCTAAGTTAGATGACGGCTATTTTACG
    TTAATTATAGTTGAAAAATCAAACCTTGCAGAACTTGGTCATATTATGACTTTAGCTTCA
    AGAGGAGAACATACTAAGCATCCTAAAGTTATTTATGAAAAAGCAAAGGCAATTAATATT
    TCATCATTCACAGACTTACAACTAAATGTAGATGGAGAATATGGTGGTAAATTACCAGCG
    AATTTCTTAAATCTAGAACGTCATATAGATGTCTTTGCACCTAATGATATTGTAAATGAA
    GAGTTAATAAATAATGATCATGTTGATGACAATTTAATCGAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    948

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1836 [new locus tag: NWMN_RS10550 ]
  • symbol: NWMN_1836
  • description: putative lipid kinase
  • length: 315
  • theoretical pI: 4.92012
  • theoretical MW: 34886.6
  • GRAVY: -0.214921

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.107?  ExPASy
    Diacylglycerol kinase (ATP) ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
  • TIGRFAM:
    Unknown function Enzymes of unknown specificity lipid kinase, YegS/Rv2252/BmrU family (TIGR00147; EC 2.7.1.-; HMM-score: 307.9)
    and 1 more
    Unknown function Enzymes of unknown specificity lipid kinase YegS (TIGR03702; EC 2.7.1.-; HMM-score: 171.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 117)
    and 1 more
    NAD_kinase; ATP-NAD kinase (PF01513; HMM-score: 21.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009172
    • TAT(Tat/SPI): 0.0006
    • LIPO(Sec/SPII): 0.000736
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKIGDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRALHIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPFAYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGYFTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGGKLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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