PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

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⊟Summary

• organism: Staphylococcus aureus Newman
locus tag: NWMN_1879 [new locus tag: NWMN_RS15465 ]
pan locus tag^?: SAUPAN005025000
symbol: NWMN_1879
pan gene symbol^?: truncated lytA
• synonym:
• product: truncated phage amidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1879 [new locus tag: NWMN_RS15465 ]
• symbol: NWMN_1879
• product: truncated phage amidase
• replicon: chromosome
• strand: -
• coordinates: 2091215..2091469
• length: 255
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331145 NCBI
• RefSeq: YP_001332913 NCBI
• BioCyc:
• MicrobesOnline: 3707467 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  
  TTGATATCCTTTTTGCCCTTCACTCGATACATATATCTCAACAACATAGAAATATTACAG
  TCGCTACACCGCATCTTAAATGGTGTGGTTATTTTTATTGGAAGTGTGTATCAGGTATCA
  GTAATGTTAAAACACCAGCTAAAAATGAAAAGAATTCACCAGTGCCAGCAGGTTATACAC
  TCGATAAAAACAATGTACCGTATAAAAAAGAGACTGGTTATTACACAGTTGCCAATGTTA
  AAGGTAATAACGTGA
  60
  120
  180
  240
  255
  

⊟Protein

⊟General

• locus tag: NWMN_1879 [new locus tag: NWMN_RS15465 ]
• symbol: NWMN_1879
• description: truncated phage amidase
• length: 84
• theoretical pI: 11.2121
• theoretical MW: 10010.2
• GRAVY: 0.397619

⊟Function

• TIGRFAM:
• TheSEED: data available for N315
• PFAM:
  SH3 (CL0010) Gemin6; Gemin6 Sm-like domain (PF06372; HMM-score: 13.4)
  no clan defined DUF1667; Protein of unknown function (DUF1667) (PF07892; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helix: 1
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7445
  • Cytoplasmic Membrane Score: 0.0752
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.18
• ⊞LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 2
  • Predicted Cleavage Site: No CleavageSite
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003588
  • TAT(Tat/SPI): 0.000311
  • LIPO(Sec/SPII): 0.000758
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151222091 NCBI
• RefSeq: YP_001332913 NCBI
• UniProt: A0A0H3K9C5 UniProt

⊟Protein sequence

• MISFLPFTRYIYLNNIEILQSLHRILNGVVIFIGSVYQVSVMLKHQLKMKRIHQCQQVIHSIKTMYRIKKRLVITQLPMLKVIT

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_1878 < NWMN_1879

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications