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NCBI: 06-JUL-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_1888 [new locus tag: NWMN_RS10865 ]
- pan locus tag?: SAUPAN005048000
- symbol: NWMN_1888
- pan gene symbol?: —
- synonym:
- product: phage tail tape measure protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: NWMN_1888 [new locus tag: NWMN_RS10865 ]
- symbol: NWMN_1888
- product: phage tail tape measure protein
- replicon: chromosome
- strand: -
- coordinates: 2101579..2106108
- length: 4530
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 5331152 NCBI
- RefSeq: YP_001332922 NCBI
- BioCyc:
- MicrobesOnline: 3707476 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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4501ATGGGAGAAAGAATAAAAGGTTTATCTATAGGTTTGGATTTAGATGCAGCAAATTTAAAT
AGATCATTTGCAGAAATCAAACGAAACTTTAAAACTTTAAATTCTGACTTAAAATTAACC
GGTAACAACTTCAAATATACCGAAAAATCAACTCATAGTTACAAACAAAGGATTAAAGAA
CTTGATGGAACTATCACAGGTTATAAGAAAAACGTTGATGATTTAGCCAAGCAATATGGC
AAGGTATCTCAAGAACAGGGCGAAAACAGCGCGGAAGCTCAAAAATTACGACAAGAATAT
AACAAACAAGCAAATGAGCTGAATTTTTTAGAAAAAGAACTAGAAAAAACAACAACTGAG
TTTGAAGAGTTCAAAAAAGCTCAAGTTGAAGCTCAAAGAATGGCAGAAAGTGGCTGGGGA
AAAACCAGTAAAGTTTTTGAAAGTATGGGACCTAAATTAACAAAAATGGGTGATGGTTTA
AAATCCATTGGTAAAGGTTTGATGATTGGTGTAACTGCACCTGTTTTAGGTATTGCAGCA
GCATCAGGAAAAGCTTTTGCAGAAGTTGATAAAGGTTTAGATACAGTTACCCAAGCAACA
GGAGCAACCGGCGGAGAGCTTAAGAAGTTGCAGAATTCATTTAAAGATGTTTATGGCAAC
TTTCCAGCAGACGCTGAGACTGTAGGCGGTGTTTTAGGGGAAGTTAACACAAGGTTAGGT
TTCACTGGCAAAGAACTTGAGAGTGCCACAGAGTCATTCTTGAAATTTAGTCACATAACA
GGTTCTGACGGCGTACAAGCCGTTCAATTAATTACGCGTGCAATGGGTGATGCAGGTATT
GAAGCTGATGAGTATCAAAGTGTACTTGATATGGTAGCGAAAGCAGCACAGGCTAGCGGT
ATAAGTGTTGATACATTAGCTGATAGCATTACTAAATACGGTGCTCCAATGAGGGCTATG
GGCTTTGAGATGAAAGAATCAATCGCTTTATTCTCTCAATGGGAGAAATCAGGTGTTAAT
ACTGAAATAGCCTTCAGTGGTTTGAAAAAAGCTATATCCAATTGGGGTAAAGCGGGTAAA
GACCCAAGAGAAGAATTTAAGAAGACATTAGCAGAAATTGAAAGGACACCGGATATAGCT
AGCGCAACAAGTTTAGCGATTGAAGCATTTGGTGCAAAAGCAGGTCCTGATTTAGCAGAT
GCTATTAAAGGCGGTCGCTTTAGTTACCAAGAGTTCTTAAAAACTATCGAAGATTCGCAA
GGAACGGTCAATCAGACATTTAAAGATTCTGAAAGTGGCTCCGAAAGATTTAAAGTAGCA
ATGAATAAACTTAAATTAGTAGGTGCTGATGTATGGGCTTCTATTGAAAGTGCGTTTGCT
CCAGTCATGGAAGAATTAATCAAAAAGCTATCTGTAGCAGTTGATTGGTTTTCAAGTTTA
AGTGATGGATCTAAAAGGTCGATTGTTATATTCGGTGGTATTGCTGCTGCAATTGGTCCT
GTAGTTTTTGGATTAGGTGCATTCATAAGCACAATTGGCAACGCAGTAACTGTATTAGCT
CCATTATTAGCTAGTATTGTAAAGGCTGGCGGATTGATTAGTTTTTTATCAACTAAAGTG
CCTATTTTAGGAACAGTCTTCACAGCATTAACTGGTCCAATTGGTATCGTGTTAGGTGTA
CTGGCTGGTTTAGCAGTCGCATTTACAATAGCTTATAAGAAATCTGAAACATTCAGAAAT
TTTGTTAATGGTGCAATTAACAGTGTTAAACAAACGTTTAGTAATTTCATTCAATTTATC
CAACCTTTCATTGATTCCGTTAAAAACGTCTTTAAACAAGCGGTTTCAGCAATCGTTGAT
TTCGCTAAAGATATTTGGAGTCAAATTAATGGATTCTTTAATGAAAACGGAATTTCTATT
GTTCAAGCGCTTCAAAATATATGCAATTTTATCAAAGCTATATTTGAATTTATCTTAAAT
TTTGTAATTAAACCAATCATGTTTGCGATTTGGCAAGTGATGCAATTTATTTGGCCGGCG
GTTAAAGCCTTGATTGTCAGTACTTGGGAGAATATAAAAGGAGTAATACAAGGTGCTTTA
AATATCATACTTGGCTTTATTAAGTTCTTTTCAAGTTTATTCACTGGTAATTGGCGAGGT
GTTTGGGACGGTATTGTGATGATACTAAAAGGCACTGTGCAGTTAATTTGGAATTTAATA
CAACTGTGGTTTGTAGGTAAGATTCTAGGTGTTGTTAGATACTTTGGTGGATTGCTTAAA
GGTTTAATATCCGGTATCTGGGGTGTTATCAAAGGTATTTTCACAAAATCATTATCTGCA
ATTTGGAATGCAACGAAAAGTATTTTTGGTTTCTTATACAATAGTGTTAAATCTATTTTC
ACTAATATGAAAAACTGGTTATCTAGTACGTGGAATAATATCAAAAGCAATACCGTCGGC
AAGGCTCATTCGTTATTTACGGGTGTAAGGTCTAAATTCACAAGTTTATGGAATGCGACG
AAAGATATATTTACTAAATTAAGAAATTGGATGTCAAACATCTGGAACTCTATTAAAGAT
AACACGGTAGGTATAGCGGGTCGCTTATGGGATAGAGTGCGTAACATCTTTGGAAGCATG
CGTGACGGTTTAAAATCTATCATTAGTAAAATTAAAGATCATATCGGTGGTATGGTAGAC
GCTGTTAAAAGAGGTCTTAATAAATTAATTGAAGGTTTAAACTGGGTCGGTGGTAAGTTG
GGTATGGACAAAATACCGAAGTTACACACTGGTACTGAACATACACATACTACTACAAGA
TTAGTTAAGAACGGTAAGATTGCACGTGACACATTCGCTACAGTTGGGGATAAGGGACGC
GGAAATGGTCCAAATGGTTTCAGAAATGAAATGATTGAATTCCCTAATGGTAAACGTGTA
ATCACACCAAATACAGATACTACGGCTTATTTACCTAAAGGCTCAAAAGTATACAACGGG
GCACAAACTTATTCAATGTTAAACGGAACGCTTCCGAGATTTAGTTTAGGTACTATGTGG
AAAGATATTAAATCTGGTGCATCATCGGCATTTAACTGGACAAAAGATAAAATAGGTAAA
GGTACCAAATGGCTTGGCGATAAAGTTGGCGATGTTTTAGATTTTATGGAAAATCCAGGC
AAACTTTTAAATTATATACTTGAAGCTTTTGGAATTGATTTCAATTCTTTAACTAAAGGT
ATGGGAATTGCAGGCGACATAACAAAAGCTGCATGGTCTAAGATTAAGAAAAGTGCTACT
GATTGGATAAAAGAAAATTTAGAAGCTATGGGCGGTGGCGATTTAGTCGGTGGAATATTA
GACCCTGACAAAATTAATTATCATTATGGACGTACCGCAGCTTATACCGCTGCAACTGGA
AGACCATTTCATGAAGGTGTCGATTTTCCATTTGTATATCAAGAAGTTAGAACGCCGATG
GGTGGCAGACTTACAAGAATGCCATTTATGTCTGGTGGTTATGGTAATTATGTAAAAATT
ACTAGTGGCGTTATCGATATGCTATTTGCGCATTTGAAAAACTTTAGCAAATCACCACCT
AGTGGCACGATGGTAAAGCCCGGTGATGTTGTTGGTTTAACTGGTAATACCGGATTTAGT
ACAGGACCACATTTACATTTTGAAATGAGGAGAAATGGAAGACATTTTGACCCTGAACCA
TATTTAAGGAATGCTAAGAAAAAAGGTAGGTTATCAATTGGTGGCGGTGGCGCTACTTCT
GGAAGTGGTGCAACTTATGCCAGCCGAGTAATCCGACAAGCACAAAGTATTTTAGGAGGA
CGTTATAAAGGTAAGTGGATTCATGACCAGATGATGCGAGTTGCAAAGCGCGAAAGTAAC
TATCAATCAAATGCAGTGAATAATTGGGACATTAATGCTCAAAGAGGAGACCCGTCTAGA
GGATTATTCCAAATTATCGGCTCAACTTTTAGAGCTAACGCTAAACGAGGGTACACTAAT
TATAATAATCCAGTACATCAAGGTATCTCAGCAATGCAGTACATTGTTAGACGATATGGT
TGGGGTGGTTTTAAACGTGCTGGTGATTACGCATATGCTACAGGTGGAAAAGTTTTTGAT
GGTTGGTATAACTTAGGTGAAGACGGTCATCCAGAATGGATTATTCCAACAGATCCAGCT
CGTAGAAATGATGCAATGAAGATTTTGCATTATGCAGCAGCAGAAGTAAGAGGGAAAAAA
GCGAGTAAAAATAAGCGTCCTAGCCAATTATCAGACTTAAACGGGTTTGATGATCCTAGC
TTATTATTGAAAATGATTGAACAACAGCAACAACAAATAGCTTTATTACTGAAAATAGCA
CAATCTAACGATGTGATTGCAGATAAAGATTATCAGCCGATTATTGACGAATACGCTTTT
GATAAAAAGGTGAACGCGTCTATAGAAAAGCGAGAAAGGCAAGAATCAACAAAAGTAAAG
TTTAGAAAAGGAGGAATTGCTATTCAATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: NWMN_1888 [new locus tag: NWMN_RS10865 ]
- symbol: NWMN_1888
- description: phage tail tape measure protein
- length: 1509
- theoretical pI: 10.2657
- theoretical MW: 165439
- GRAVY: -0.203048
⊟Function[edit | edit source]
- TIGRFAM: Mobile and extrachromosomal element functions Prophage functions phage tail tape measure protein, TP901 family, core region (TIGR01760; HMM-score: 65.3)and 11 moretwo transmembrane protein (TIGR04527; HMM-score: 26.1)Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 24.3)DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 24.3)Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 23.8)DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 23.8)Unknown function Enzymes of unknown specificity quinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 13.6)DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 13)Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 13)Protein synthesis tRNA aminoacylation serine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 11.7)phasin (TIGR01985; HMM-score: 8.8)SH3 domain protein (TIGR04211; HMM-score: 8.1)
- TheSEED: data available for N315, USA300_FPR3757
- PFAM: no clan defined PhageMin_Tail; Phage-related minor tail protein (PF10145; HMM-score: 66.9)Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 61.9)and 27 moreLysozyme (CL0037) SLT; Transglycosylase SLT domain (PF01464; HMM-score: 25.6)no clan defined DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 17.7)GAS; Growth-arrest specific micro-tubule binding (PF13851; HMM-score: 17.6)TMP_2; Prophage tail length tape measure protein (PF06791; HMM-score: 17.1)TPR_MLP1_2; TPR/MLP1/MLP2-like protein (PF07926; HMM-score: 16.7)Goodbye; fungal STAND N-terminal Goodbye domain (PF17109; HMM-score: 16)EsxAB (CL0352) WXG100; Proteins of 100 residues with WXG (PF06013; HMM-score: 14.4)no clan defined YtxH; YtxH-like protein (PF12732; HMM-score: 14.4)Baculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 14.2)EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 14)Laminin_I; Laminin Domain I (PF06008; HMM-score: 13.5)FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 13.4)BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 12.9)no clan defined DUF745; Protein of unknown function (DUF745) (PF05335; HMM-score: 12.4)TACC; Transforming acidic coiled-coil-containing protein (TACC) (PF05010; HMM-score: 12.1)TMPIT; TMPIT-like protein (PF07851; HMM-score: 11.5)HTH (CL0123) Mnd1; Mnd1 family (PF03962; HMM-score: 11.2)no clan defined Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 10.1)BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 10)DUF16; Protein of unknown function DUF16 (PF01519; HMM-score: 9.8)tRNA_bind_arm (CL0298) Seryl_tRNA_N; Seryl-tRNA synthetase N-terminal domain (PF02403; HMM-score: 9.7)no clan defined IFT57; Intra-flagellar transport protein 57 (PF10498; HMM-score: 9.6)OmpH; Outer membrane protein (OmpH-like) (PF03938; HMM-score: 9.1)Phage_GPO; Phage capsid scaffolding protein (GPO) serine peptidase (PF05929; HMM-score: 8.8)Tho2; Transcription factor/nuclear export subunit protein 2 (PF11262; HMM-score: 7.8)Carbpep_Y_N; Carboxypeptidase Y pro-peptide (PF05388; HMM-score: 6.6)DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 6.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 9.35
- Cellwall Score: 0.32
- Extracellular Score: 0.32
- Internal Helices: 6
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.020835
- TAT(Tat/SPI): 0.002675
- LIPO(Sec/SPII): 0.001517
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNFKYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNKQANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGLKSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVYGNFPADAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSDGVQAVQLITRAMGDAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQWEKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGAKAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGADVWASIESAFAPVMEELIKKLSVAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAFISTIGNAVTVLAPLLASIVKAGGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAVAFTIAYKKSETFRNFVNGAINSVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKDIWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVKALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLIQLWFVGKILGVVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKSIFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWNSIKDNTVGIAGRLWDRVRNIFGSMRDGLKSIISKIKDHIGGMVDAVKRGLNKLIEGLNWVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMIEFPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSLGTMWKDIKSGASSAFNWTKDKIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDITKAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEGVDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTMVKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGSGATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSRGLFQIIGSTFRANAKRGYTNYNNPVHQGISAMQYIVRRYGWGGFKRAGDYAYATGGKVFDGWYNLGEDGHPEWIIPTDPARRNDAMKILHYAAAEVRGKKASKNKRPSQLSDLNGFDDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQESTKVKFRKGGIAIQ
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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