PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1988 [new locus tag: NWMN_RS11500 ]
• pan locus tag^?: SAUPAN005357000
• symbol: NWMN_1988
• pan gene symbol^?: rodA
• synonym:
• product: cell division protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1988 [new locus tag: NWMN_RS11500 ]
• symbol: NWMN_1988
• product: cell division protein
• replicon: chromosome
• strand: +
• coordinates: 2210624..2211826
• length: 1203
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331856 NCBI
• RefSeq: YP_001333022 NCBI
• BioCyc:
• MicrobesOnline: 3707581 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGAATTATTCATCTCGTCAACAGCCGGATAAGCATTGGCTTCGCAAAGTAGACTGGGTA
  TTAGTAGCCACTATAGCTGTTTTAGCAATTTTCAGTGTTCTGCTTATTAACTCGGCAATG
  GGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGT
  GCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATAT
  TTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCT
  ATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAG
  CCATCTGAATTCATGAAAATTATTTTAATTTTAGCATTAGCGCGTGTCGTTTCTAGACAT
  AATCAATTCACATTCAATAAATCATTCCAAAGTGATTTGTTATTATTTTTCAAAATTATT
  GGTGTCTCGTTAGTACCAAGTATTTTAATATTACTGCAAAATGACCTAGGAACTACATTA
  GTATTAGCTGCTATTATTGCAGGTGTGATGTTAGTAAGTGGTATAACATGGCGTATCTTA
  GCACCTATCTTTATTACAGGTATTGTTGGTGCAATGACAGTCATTTTAGGTATTCTATAT
  GCACCCGCATTAATTGAAAATTTATTAGGTGTCCAACTGTATCAAATGGGACGAATCAAT
  TCATGGCTTGACCCCTATACATATAGTAGTGGTGATGGCTATCATTTAACTGAATCACTT
  AAAGCTATCGGTTCTGGACAGTTACTAGGTAAAGGATACAATCACGGTGAAGTTTATATA
  CCTGAAAATCATACTGACTTTATCTTTTCAGTGATTGGAGAGGAACTTGGCTTTATCGGT
  TCTGTCATATTGATCTTAATATTTTTATTTTTAATCTTCCATCTAATAAGATTAGCTGCG
  AAAATTGAAGATCAATTTAACAAAATCTTTATCGTTGGTTTCGTCACTTTACTTGTGTTC
  CATATTTTACAAAATATTGGTATGACAATTCAGTTGTTACCAATCACTGGTATTCCATTA
  CCATTTATTAGTTATGGTGGTAGTGCGCTATGGAGTATGATGACTGGAATAGGTATAGTC
  TTATCAATTTATTATCATGAACCAAAACGATATGTCGATTTATACCATCCAAAAAGTAAT
  TAA
  60
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  1140
  1200
  1203
  

⊟Protein

⊟General

• locus tag: NWMN_1988 [new locus tag: NWMN_RS11500 ]
• symbol: NWMN_1988
• description: cell division protein
• length: 400
• theoretical pI: 9.31632
• theoretical MW: 44600.8
• GRAVY: 0.765

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein RodA (TIGR02210; HMM-score: 326.2)
  Cellular processes Cellular processes Cell division rod shape-determining protein RodA (TIGR02210; HMM-score: 326.2)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan cell division protein FtsW (TIGR02614; HMM-score: 273.1)
  Cellular processes Cellular processes Cell division cell division protein FtsW (TIGR02614; HMM-score: 273.1)
  and 2 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein E (TIGR02615; HMM-score: 199.3)
  Cellular processes Cellular processes Sporulation and germination stage V sporulation protein E (TIGR02615; HMM-score: 199.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Membrane_trans (CL0142) FTSW_RODA_SPOVE; Cell cycle protein (PF01098; HMM-score: 319.3)
  and 1 more
  no clan defined Clostridium_P47; Clostridium P-47 protein (PF06597; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022773
  • TAT(Tat/SPI): 0.000872
  • LIPO(Sec/SPII): 0.025839
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222200 NCBI
• RefSeq: YP_001333022 NCBI
• UniProt: A0A0H3K9L0 UniProt

⊟Protein sequence

• MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQYSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPITPIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKIIGVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGILYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHGEVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGFVTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYVDLYHPKSN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications