From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2075 [new locus tag: SACOL_RS10855 ]
  • pan locus tag?: SAUPAN005357000
  • symbol: ftsW
  • pan gene symbol?: rodA
  • synonym:
  • product: cell cycle protein FtsW

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2075 [new locus tag: SACOL_RS10855 ]
  • symbol: ftsW
  • product: cell cycle protein FtsW
  • replicon: chromosome
  • strand: +
  • coordinates: 2142241..2143443
  • length: 1203
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATTATTCATCTCGTCAACAGCCGGATAAGCATTGGCTTCGCAAAGTAGACTGGGTA
    TTAGTAGCCACTATAGCTGTTTTAGCAATTTTCAGTGTTCTGCTTATTAACTCGGCAATG
    GGCGGTGGTCAATACAGTGCTAATTTCGGTATCAGACAAATTTTTTATTACATTTTAGGT
    GCAATTTTTGCAGGTATCATCATGTTTATTTCACCTAAAAAGATTAAACATTATACATAT
    TTATTGTATTTCTTAATCTGTCTATTATTAATAGGCTTGCTCGTTATTCCTGAGTCACCT
    ATTACACCTATTATCAATGGTGCCAAAAGTTGGTACACGTTTGGCCCTATCAGTATTCAG
    CCATCTGAATTCATGAAAATTATTTTAATTTTAGCATTAGCGCGTGTCGTTTCTAGACAT
    AATCAATTCACATTCAATAAATCATTCCAAAGTGATTTGTTATTATTTTTCAAAATTATT
    GGTGTCTCGTTAGTACCAAGTATTTTAATATTACTGCAAAATGACCTAGGAACTACATTA
    GTATTAGCTGCTATTATTGCAGGTGTGATGTTAGTAAGTGGTATAACATGGCGTATCTTA
    GCACCTATCTTTATTACAGGTATTGTTGGTGCAATGACAGTCATTTTAGGTATTCTATAT
    GCACCCGCATTAATTGAAAATTTATTAGGTGTCCAACTGTATCAAATGGGACGAATCAAT
    TCATGGCTTGACCCCTATACATATAGTAGTGGTGATGGCTATCATTTAACTGAATCACTT
    AAAGCTATCGGTTCTGGACAGTTACTAGGTAAAGGATACAATCACGGTGAAGTTTATATA
    CCTGAAAATCATACTGACTTTATCTTTTCAGTGATTGGAGAGGAACTTGGCTTTATCGGT
    TCTGTCATATTGATCTTAATATTTTTATTTTTAATCTTCCATCTAATAAGATTAGCTGCG
    AAAATTGAAGATCAATTTAACAAAATCTTTATCGTTGGTTTCGTCACTTTACTTGTGTTC
    CATATTTTACAAAATATTGGTATGACAATTCAGTTGTTACCAATCACTGGTATTCCATTA
    CCATTTATTAGTTATGGTGGTAGTGCGCTATGGAGTATGATGACTGGAATAGGTATAGTC
    TTATCAATTTATTATCATGAACCAAAACGATATGTCGATTTATACCATCCAAAAAGTAAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2075 [new locus tag: SACOL_RS10855 ]
  • symbol: FtsW
  • description: cell cycle protein FtsW
  • length: 400
  • theoretical pI: 9.31632
  • theoretical MW: 44600.8
  • GRAVY: 0.765

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan rod shape-determining protein RodA (TIGR02210; HMM-score: 326.2)
    Cellular processes Cellular processes Cell division rod shape-determining protein RodA (TIGR02210; HMM-score: 326.2)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan cell division protein FtsW (TIGR02614; HMM-score: 273.1)
    Cellular processes Cellular processes Cell division cell division protein FtsW (TIGR02614; HMM-score: 273.1)
    and 2 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein E (TIGR02615; HMM-score: 199.3)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein E (TIGR02615; HMM-score: 199.3)
  • TheSEED  :
    • Peptidoglycan glycosyltransferase MrdB (EC 2.4.1.129)
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsW
  • PFAM:
    Membrane_trans (CL0142) FTSW_RODA_SPOVE; Cell cycle protein (PF01098; HMM-score: 319.3)
    and 1 more
    no clan defined Clostridium_P47; Clostridium P-47 protein (PF06597; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.022773
    • TAT(Tat/SPI): 0.000872
    • LIPO(Sec/SPII): 0.025839
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQYSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPITPIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKIIGVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGILYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHGEVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGFVTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYVDLYHPKSN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2075&oldid=99188"