PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1992 [new locus tag: NWMN_RS11520 ]
• pan locus tag^?: SAUPAN005364000
• symbol: cls
• pan gene symbol^?: cls2
• synonym:
• product: cardiolipin synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1992 [new locus tag: NWMN_RS11520 ]
• symbol: cls
• product: cardiolipin synthetase
• replicon: chromosome
• strand: +
• coordinates: 2213035..2214519
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331222 NCBI
• RefSeq: YP_001333026 NCBI
• BioCyc:
• MicrobesOnline: 3707585 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATAGAGTTATTATCCATTGCACTCAAGCATTCTAATATTATTTTAAATTCAATATTT
  ATTGGTGCATTTATTTTAAACTTATTATTCGCCTTTACCATTATTTTCATGGAAAGACGT
  TCTGCCAATTCTATCTGGGCTTGGTTACTAGTCTTAGTTTTCTTGCCTTTATTCGGCTTC
  ATTTTATACTTACTATTAGGACGACAAATTCAACGTGACCAAATTTTCAAAATTGATAAG
  GAAGATAAAAAAGGATTAGAGTTAATCGTTGATGAGCAATTAGCTGCTTTAAAAAATGAA
  AACTTTTCAAATTCCAATTATCAAATTGTAAAATTTAAAGAAATGATTCAAATGTTGTTA
  TATAATAACGCAGCATTTTTAACAACAGACAACGATTTAAAAATATACACAGACGGCCAA
  GAAAAATTTGATGACCTAATACAAGACATCCGTAATGCTACTGATTATATTCATTTTCAG
  TACTATATTATTCAAAATGATGAATTAGGTCGTACCATTTTAAATGAACTTGGTAAAAAA
  GCGGAACAAGGTGTAGAAGTTAAAATTCTTTATGATGACATGGGTTCTCGTGGACTGCGT
  AAAAAAGGCTTACGCCCGTTTCGCAATAAAGGTGGACATGCTGAAGCATTTTTCCCATCA
  AAATTACCTTTAATTAACTTGCGTATGAACAATCGAAACCATCGAAAAATTGTTGTAATA
  GATGGGCAAATTGGATATGTTGGTGGTTTTAATGTTGGTGATGAGTACTTAGGTAAATCA
  AAAAAATTCGGCTATTGGCGAGATACGCATTTACGAATTGTCGGGGATGCAGTGAATGCA
  TTGCAATTACGATTTATTCTAGATTGGAATTCACAAGCCACACGTGACCACATCTCCTAT
  GATGATCGTTATTTCCCAGATGTAAATTCTGGTGGAACAATTGGCGTTCAAATAGCTTCT
  AGTGGTCCTGACGAAGAATGGGAACAGATTAAATACGGCTATTTGAAAATGATTTCATCT
  GCTAAAAAATCGATTTATATTCAATCTCCCTATTTCATACCTGATCAAGCCTTTTTAGAT
  TCTATTAAAATTGCGGCATTAGGTGGTGTTGATGTCAATATCATGATTCCTAATAAACCT
  GACCATCCGTTTGTTTTTTGGGCTACTTTAAAAAATGCAGCATCCTTATTAGATGCCGGT
  GTTAAAGTATTTCACTACGACAATGGCTTTTTACACTCAAAAACACTTGTTATAGATGAT
  GAAATTGCAAGTGTGGGAACAGCTAATATGGACCATCGCAGTTTCACATTGAATTTCGAA
  GTCAACGCTTTTATTTATGACCAACAAATTGCCAAAAAATTAAAACAAGCTTTTATAGAT
  GATTTAGCAGTATCTTCTGAATTAACAAAAGCACGTTATGCTAAGCGAAGTCTTTGGATT
  AAATTTAAAGAAGGTATTTCACAATTATTGTCACCTATCTTATAA
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⊟Protein

⊟General

• locus tag: NWMN_1992 [new locus tag: NWMN_RS11520 ]
• symbol: Cls
• description: cardiolipin synthetase
• length: 494
• theoretical pI: 8.49192
• theoretical MW: 56517.7
• GRAVY: -0.0979757

⊟Function

• reaction:
  EC 2.7.8.-?  ExPASy
  2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
• TIGRFAM:
  cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 821.2)
  and 2 more
  Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.9)
  Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 157.1)
  and 2 more
  PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 58.5)
  no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 43.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000568
  • TAT(Tat/SPI): 0.000123
  • LIPO(Sec/SPII): 0.028922
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 151222204 NCBI
• RefSeq: YP_001333026 NCBI
• UniProt: A0A0H3KHY3 UniProt

⊟Protein sequence

• MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cls > NWMN_1993

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications