PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2044 [new locus tag: SAUSA300_RS11245 ]
• pan locus tag^?: SAUPAN005364000
• symbol: cls
• pan gene symbol^?: cls2
• synonym:
• product: cardiolipin synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2044 [new locus tag: SAUSA300_RS11245 ]
• symbol: cls
• product: cardiolipin synthetase
• replicon: chromosome
• strand: +
• coordinates: 2208379..2209938
• length: 1560
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912807 NCBI
• RefSeq: YP_494690 NCBI
• BioCyc: see SAUSA300_RS11245
• MicrobesOnline: 1293559 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATATTTAAATTGCTTCATGATACAATTAATAAGATATACTACTCGTTTAAGATTTTC
  GATGGGAGGTTTCAAATGATAGAGTTATTATCCATTGCACTCAAGCATTCTAATATTATT
  TTAAATTCAATATTTATTGGTGCATTTATTTTAAACTTATTATTCGCCTTTACCATTATT
  TTCATGGAAAGACGTTCTGCCAATTCTATCTGGGCTTGGTTACTAGTCTTAGTTTTCTTG
  CCTTTATTCGGCTTCATTTTATACTTACTATTAGGACGACAAATTCAACGTGACCAAATT
  TTCAAAATTGATAAGGAAGATAAAAAAGGATTAGAGTTAATCGTTGATGAGCAATTAGCT
  GCTTTAAAAAATGAAAACTTTTCAAATTCCAATTATCAAATTGTAAAATTTAAAGAAATG
  ATTCAAATGTTGTTATATAATAACGCAGCATTTTTAACAACAGACAACGATTTAAAAATA
  TACACAGACGGCCAAGAAAAATTTGATGACCTAATACAAGACATCCGTAATGCTACTGAT
  TATATTCATTTTCAGTACTATATTATTCAAAATGATGAATTAGGTCGTACCATTTTAAAT
  GAACTTGGTAAAAAAGCGGAACAAGGTGTAGAAGTTAAAATTCTTTATGATGACATGGGT
  TCTCGTGGACTGCGTAAAAAAGGCTTACGCCCGTTTCGCAATAAAGGTGGACATGCTGAA
  GCATTTTTCCCATCAAAATTACCTTTAATTAACTTGCGTATGAACAATCGAAACCATCGA
  AAAATTGTTGTAATAGATGGGCAAATTGGATATGTTGGTGGTTTTAATGTTGGTGATGAG
  TACTTAGGTAAATCAAAAAAATTCGGCTATTGGCGAGATACGCATTTACGAATTGTCGGG
  GATGCAGTGAATGCATTGCAATTACGATTTATTCTAGATTGGAATTCACAAGCCACACGT
  GACCACATCTCCTATGATGATCGTTATTTCCCAGATGTAAATTCTGGTGGAACAATTGGC
  GTTCAAATAGCTTCTAGTGGTCCTGACGAAGAATGGGAACAGATTAAATACGGCTATTTG
  AAAATGATTTCATCTGCTAAAAAATCGATTTATATTCAATCTCCCTATTTCATACCTGAT
  CAAGCCTTTTTAGATTCTATTAAAATTGCGGCATTAGGTGGTGTTGATGTCAATATCATG
  ATTCCTAATAAACCTGACCATCCGTTTGTTTTTTGGGCTACTTTAAAAAATGCAGCATCC
  TTATTAGATGCCGGTGTTAAAGTATTTCACTACGACAATGGCTTTTTACACTCAAAAACA
  CTTGTTATAGATGATGAAATTGCAAGTGTGGGAACAGCTAATATGGACCATCGCAGTTTC
  ACATTGAATTTCGAAGTCAACGCTTTTATTTATGACCAACAAATTGCCAAAAAATTAAAA
  CAAGCTTTTATAGATGATTTAGCAGTATCTTCTGAATTAACAAAAGCACGTTATGCTAAG
  CGAAGTCTTTGGATTAAATTTAAAGAAGGTATTTCACAATTATTGTCACCTATCTTATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2044 [new locus tag: SAUSA300_RS11245 ]
• symbol: Cls
• description: cardiolipin synthetase
• length: 519
• theoretical pI: 9.01273
• theoretical MW: 59638.4
• GRAVY: -0.0917148

⊟Function

• reaction:
  EC 2.7.8.-?  ExPASy
  2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
• TIGRFAM:
  cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 821)
  and 2 more
  Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.7)
  Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.7)
• TheSEED  :
  • Cardiolipin synthase, bacterial type ClsA
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Cardiolipin synthetase (EC 2.7.8.-)
• PFAM:
  PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 156.8)
  and 2 more
  PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 58.3)
  no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 43)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helices: 2
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000457
  • TAT(Tat/SPI): 0.000058
  • LIPO(Sec/SPII): 0.001635
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 87160381 NCBI
• RefSeq: YP_494690 NCBI
• UniProt: A0A0H2XGI3 UniProt

⊟Protein sequence

• MIFKLLHDTINKIYYSFKIFDGRFQMIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cls > SAUSA300_2045

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications