PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS11245 [old locus tag: SAUSA300_2044 ]
• pan locus tag^?: SAUPAN005364000
• symbol: SAUSA300_RS11245
• pan gene symbol^?: cls2
• synonym:
• product: cardiolipin synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS11245 [old locus tag: SAUSA300_2044 ]
• symbol: SAUSA300_RS11245
• product: cardiolipin synthase
• replicon: chromosome
• strand: +
• coordinates: 2208454..2209938
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2208454..2209938) NCBI
• BioCyc: SAUSA300_RS11245 BioCyc
• MicrobesOnline: see SAUSA300_2044

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGATAGAGTTATTATCCATTGCACTCAAGCATTCTAATATTATTTTAAATTCAATATTT
  ATTGGTGCATTTATTTTAAACTTATTATTCGCCTTTACCATTATTTTCATGGAAAGACGT
  TCTGCCAATTCTATCTGGGCTTGGTTACTAGTCTTAGTTTTCTTGCCTTTATTCGGCTTC
  ATTTTATACTTACTATTAGGACGACAAATTCAACGTGACCAAATTTTCAAAATTGATAAG
  GAAGATAAAAAAGGATTAGAGTTAATCGTTGATGAGCAATTAGCTGCTTTAAAAAATGAA
  AACTTTTCAAATTCCAATTATCAAATTGTAAAATTTAAAGAAATGATTCAAATGTTGTTA
  TATAATAACGCAGCATTTTTAACAACAGACAACGATTTAAAAATATACACAGACGGCCAA
  GAAAAATTTGATGACCTAATACAAGACATCCGTAATGCTACTGATTATATTCATTTTCAG
  TACTATATTATTCAAAATGATGAATTAGGTCGTACCATTTTAAATGAACTTGGTAAAAAA
  GCGGAACAAGGTGTAGAAGTTAAAATTCTTTATGATGACATGGGTTCTCGTGGACTGCGT
  AAAAAAGGCTTACGCCCGTTTCGCAATAAAGGTGGACATGCTGAAGCATTTTTCCCATCA
  AAATTACCTTTAATTAACTTGCGTATGAACAATCGAAACCATCGAAAAATTGTTGTAATA
  GATGGGCAAATTGGATATGTTGGTGGTTTTAATGTTGGTGATGAGTACTTAGGTAAATCA
  AAAAAATTCGGCTATTGGCGAGATACGCATTTACGAATTGTCGGGGATGCAGTGAATGCA
  TTGCAATTACGATTTATTCTAGATTGGAATTCACAAGCCACACGTGACCACATCTCCTAT
  GATGATCGTTATTTCCCAGATGTAAATTCTGGTGGAACAATTGGCGTTCAAATAGCTTCT
  AGTGGTCCTGACGAAGAATGGGAACAGATTAAATACGGCTATTTGAAAATGATTTCATCT
  GCTAAAAAATCGATTTATATTCAATCTCCCTATTTCATACCTGATCAAGCCTTTTTAGAT
  TCTATTAAAATTGCGGCATTAGGTGGTGTTGATGTCAATATCATGATTCCTAATAAACCT
  GACCATCCGTTTGTTTTTTGGGCTACTTTAAAAAATGCAGCATCCTTATTAGATGCCGGT
  GTTAAAGTATTTCACTACGACAATGGCTTTTTACACTCAAAAACACTTGTTATAGATGAT
  GAAATTGCAAGTGTGGGAACAGCTAATATGGACCATCGCAGTTTCACATTGAATTTCGAA
  GTCAACGCTTTTATTTATGACCAACAAATTGCCAAAAAATTAAAACAAGCTTTTATAGAT
  GATTTAGCAGTATCTTCTGAATTAACAAAAGCACGTTATGCTAAGCGAAGTCTTTGGATT
  AAATTTAAAGAAGGTATTTCACAATTATTGTCACCTATCTTATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1485
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS11245 [old locus tag: SAUSA300_2044 ]
• symbol: SAUSA300_RS11245
• description: cardiolipin synthase
• length: 494
• theoretical pI: 8.49192
• theoretical MW: 56517.7
• GRAVY: -0.0979757

⊟Function

• reaction:
  EC 2.7.8.-?  ExPASy
  2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol?
• TIGRFAM:
  cardiolipin synthase (TIGR04265; EC 2.7.8.-; HMM-score: 821.2)
  and 2 more
  Genetic information processing Protein fate Protein folding and stabilization cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.9)
  Metabolism Energy metabolism Electron transport cytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 10.9)
• TheSEED: see SAUSA300_2044
• PFAM:
  PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 157.1)
  and 2 more
  PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 58.5)
  no clan defined PLDc_N; Phospholipase_D-nuclease N-terminal (PF13396; HMM-score: 43.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000568
  • TAT(Tat/SPI): 0.000123
  • LIPO(Sec/SPII): 0.028922
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: 446493695 NCBI
• RefSeq: WP_000571549 NCBI
• UniProt: see SAUSA300_2044

⊟Protein sequence

• MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANSIWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENFSNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQYYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFFPSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGDAVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGYLKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKNAASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQIAKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications