PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2025 [new locus tag: NWMN_RS11700 ]
• pan locus tag^?: SAUPAN005413000
• symbol: rho
• pan gene symbol^?: rho
• synonym:
• product: transcription termination factor Rho

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2025 [new locus tag: NWMN_RS11700 ]
• symbol: rho
• product: transcription termination factor Rho
• replicon: chromosome
• strand: -
• coordinates: 2240633..2241949
• length: 1317
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332393 NCBI
• RefSeq: YP_001333059 NCBI
• BioCyc:
• MicrobesOnline: 3707618 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCCTGAAAGAGAACGTACATCTCCTCAGTATGAATCATTCCACGAATTGTACAAGAAC
  TATACTACCAAGGAACTCACTCAAAAAGCTAAAACTCTTAAGTTGACGAACTATAGTAAA
  TTAAATAAAAAAGAACTTGTTCTAGCTATTATGGAAGCACAAATGGAAAAAGATGGTAAC
  TATTATATGGAAGGTATCTTAGATGATATACAACCAGATGGTTATGGTTTTTTAAGAACA
  GTGAACTATTCTAAAGGGGAAAAAGATATTTATATATCTGCTAGCCAAATTCGTCGTTTT
  GAAATTAAACGTGGGGATAAAGTAACTGGGAAAGTTAGAAAACCTAAAGATAACGAAAAA
  TATTATGGCTTATTACAAGTTGACTTTGTCAATGACCATAACGCAGAAGAAGTGAAGAAA
  CGTCCGCATTTCCAAGCTTTGACACCACTTTATCCAGATGAGCGTATTAAATTAGAGACA
  GAAACACAAAATTATTCAACGCGCATCATGGATTTAGTAACACCGATTGGTTTAGGTCAA
  CGTGGTTTAATAGTGGCGCCACCTAAAGCAGGTAAAACATCGTTATTAAAAGAAATAGCG
  AATGCAATCAGTACGAACAAACCAGATGCAAAGCTATTTATTTTGTTAGTTGGCGAGCGT
  CCTGAAGAGGTAACAGATTTAGAACGCTCAGTAGAAGCTGCTGAAGTCGTTCATTCAACG
  TTTGACGAACCACCAGAACACCATGTTAAAGTAGCTGAATTATTACTTGAACGTGCAAAG
  CGTTTAGTAGAAATTGGGGAAGATGTCATTATTTTAATGGATTCTATAACGAGATTAGCA
  CGCGCTTATAACTTAGTTATTCCACCAAGTGGTCGTACATTATCAGGTGGTTTAGATCCT
  GCATCTTTACACAAACCAAAAGCATTCTTCGGTGCAGCGAGAAATATTGAAGCGGGTGGA
  AGTTTAACAATACTTGCAACTGCATTAGTTGATACGGGTTCACGTATGGACGATATGATT
  TACGAAGAATTTAAAGGAACAGGTAACATGGAGTTACATTTAGATCGTAAATTGTCTGAA
  CGTCGTATCTTCCCTGCAATTGATATTGGCAGAAGTTCAACGCGTAAAGAAGAATTGTTG
  ATAAGTAAATCTGAATTAGACACATTATGGCAATTAAGAAATCTATTCACTGACTCAACT
  GACTTTACTGAAAGATTTATTCGCAAACTTAAAAGGTCTAAGAATAATGAAGATTTCTTC
  AAGCAGCTACAAAAGTCTGCAGAAGAAAGTACTAAAACGGGTCGACCTATAATTTAA
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⊟Protein

⊟General

• locus tag: NWMN_2025 [new locus tag: NWMN_RS11700 ]
• symbol: Rho
• description: transcription termination factor Rho
• length: 438
• theoretical pI: 8.53546
• theoretical MW: 49957.7
• GRAVY: -0.581279

⊟Function

• reaction:
  EC 3.6.4.-?  ExPASy
• TIGRFAM:
  Genetic information processing Transcription Transcription factors transcription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 622.7)
  and 11 more
  Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 83.8)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 83.7)
  Cellular processes Cellular processes Pathogenesis type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 83.1)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 83.1)
  flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 82.9)
  Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 73.8)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 36.8)
  alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 33)
  alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 25.3)
  Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AA (TIGR02858; HMM-score: 24.2)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 19.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  OB (CL0021) Rho_RNA_bind; Rho termination factor, RNA-binding domain (PF07497; HMM-score: 98.1)
  and 9 more
  P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 75.6)
  HeH (CL0306) Rho_N; Rho termination factor, N-terminal domain (PF07498; HMM-score: 44.2)
  P-loop_NTPase (CL0023) NACHT; NACHT domain (PF05729; HMM-score: 25.2)
  OB (CL0021) OB_RNB; Ribonuclease B OB domain (PF08206; HMM-score: 21.2)
  P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16)
  AAA_25; AAA domain (PF13481; HMM-score: 15.5)
  HTH (CL0123) Mor; Mor transcription activator family (PF08765; HMM-score: 14.8)
  P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 13.8)
  AAA_14; AAA domain (PF13173; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002608
  • TAT(Tat/SPI): 0.000357
  • LIPO(Sec/SPII): 0.000255
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222237 NCBI
• RefSeq: YP_001333059 NCBI
• UniProt: A0A0H3KHZ8 UniProt

⊟Protein sequence

• MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

R S Washburn, A Marra, A P Bryant, M Rosenberg, D R Gentry
rho is not essential for viability or virulence in Staphylococcus aureus.
Antimicrob Agents Chemother: 2001, 45(4);1099-103
[PubMed:11257021] [WorldCat.org] [DOI] (P p)