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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2044 [new locus tag: NWMN_RS11805 ]
  • pan locus tag?: SAUPAN005437000
  • symbol: NWMN_2044
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2044 [new locus tag: NWMN_RS11805 ]
  • symbol: NWMN_2044
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2261298..2261711
  • length: 414
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGCCAATCGTATATTATGATGGAAACTGTATATATTGTTATAACTATGTCATTTGGTTA
    ATTCAACATGACCTACCAAGAAATTATCAATTTGCCACGTTAAAAGGTGAAGTTGGCCAA
    CAATTTTTTAAACAACATCCAGAAGCGGCAAATAAAAATAGTGTGATTTTACAGAAAGGT
    GATCGTATATATTTTGAATCGCAGGCGATTATTAAACTAATCACAGCACTACCTAATCGA
    ACTAAATTATTGGGCGTAGGTTTATGGCTTGTACCTAAACCAGTCCGTAATTTCGGTTAT
    CGAATGTTTGCTAATAATAGAAATAGAATGTGGAAAACAACATGGCACCAACCAAATGAT
    TATGAAAAATCGTTCTTTTTAGATGATAATGCGAAAGTAAAACTTACTGATTGA
    60
    120
    180
    240
    300
    360
    414

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2044 [new locus tag: NWMN_RS11805 ]
  • symbol: NWMN_2044
  • description: hypothetical protein
  • length: 137
  • theoretical pI: 9.81923
  • theoretical MW: 16253.6
  • GRAVY: -0.459854

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined DUF393; Protein of unknown function, DUF393 (PF04134; HMM-score: 75.9)
    and 1 more
    DUF295; Protein of unknown function (DUF295) (PF03478; HMM-score: 7.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005967
    • TAT(Tat/SPI): 0.000154
    • LIPO(Sec/SPII): 0.000961
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPIVYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFKQHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRMFANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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