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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2069 [new locus tag: NWMN_RS11990 ]
  • pan locus tag?: SAUPAN005555000
  • symbol: NWMN_2069
  • pan gene symbol?: sepA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2069 [new locus tag: NWMN_RS11990 ]
  • symbol: NWMN_2069
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2305522..2305995
  • length: 474
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGATTGTGAATTATCTTAAACATAAGTTTTATAACTTGTTAACTACAATGATTGTTCTC
    TTTATTTTTGTACTTTCTGGTGCGATTTTTTTAACATTTCTAGGGTTTGGTTTATATGGA
    TTAAGTAGAATACTTATTTATTTTAGGTTGGGTGACTTTACATATAATAGAAGTATGTAC
    GATAACCTATTATATTATGGCAGTTATATAATCTTTGGGTACTTCATCATATTTGCAGTC
    GAGCATTTAATGGATTATTTTAGAAAAATGTTGCCTGAAAATGCATATTTTAGAGGCGCG
    ACTTTTCATTTGATTTCTTACACAGTTGCAACAACGTTATTTTATTTTATTATTCATCTG
    AACTATGTGTATATCAATATCGATTTTTGGGTCATCATGGTTATTATAGGGTTCTTATAT
    GTTTGTAAGCTTCAATTTTACCCTGAAAGTAAAAATTTAAATAATAGAAAATAG
    60
    120
    180
    240
    300
    360
    420
    474

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2069 [new locus tag: NWMN_RS11990 ]
  • symbol: NWMN_2069
  • description: hypothetical protein
  • length: 157
  • theoretical pI: 9.40365
  • theoretical MW: 18899.3
  • GRAVY: 0.681529

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined SepA; Multidrug Resistance efflux pump (PF17080; HMM-score: 198)
    and 1 more
    DUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002375
    • TAT(Tat/SPI): 0.000374
    • LIPO(Sec/SPII): 0.032554
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRILIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATFHLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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