PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2091 [new locus tag: NWMN_RS12105 ]
• pan locus tag^?: SAUPAN005583000
• symbol: NWMN_2091
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2091 [new locus tag: NWMN_RS12105 ]
• symbol: NWMN_2091
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2328467..2329468
• length: 1002
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331283 NCBI
• RefSeq: YP_001333125 NCBI
• BioCyc:
• MicrobesOnline: 3707693 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCAAAATAAACAAATTCTTTTTAACAAAATACCAGAAGGCATGCCTCAAGAGGATACA
  TTCAAAATTGAAGAGATTGACACGCCTAAACTTGAGAGCGATGGTGTACTTGTACAAACG
  CTGTATATTTCAGTGGATCCATATATGAGAGGACGTATGACTAAGGCAGATTCGTATGTA
  CAGCCTTTTGAAATTGGTAAACCGATTGTTAGCCATGTCGTTGCAAAAGTAATTGATTCG
  ACACTCGCAGATTATAAAAAAGGAGATGTCGTTGTAGGTATGTTGCCTTGGCGAATCATT
  AATCATGTCCAAGCGGATCAAATTACTAAAGTTCCAACGACAGATGTACCACTTGATTTA
  TATTTAAGTGTTTTAGGAATGCCAGGACAAACAGCATATCATGGTCTGCTTGATATTGGA
  CAACCTAAAGCCGGTGATACTGTTGTGGTTTCAGCTGCATCTGGTGCTGTAGGTTCAGTT
  GTAGGTCAAATTGCTAAGATTAAAGGTTGCCGTGTAGTAGGTATTGCGGGTGGCGACAAA
  AAAGTAAATTATTTAACAGAGACACTGGGCTTTGACGCTGGTATTGATTATAAGAAAGAG
  GATTTTGCAGAAGCGTTAGCACAAGCAGTACCAGATGGTATTGATGTTTATTTTGAAAAT
  GTTGGTGGAACAGTTGGCGATGAAGTATTTAAGCATTTAAATCGTTTTGCACGTGTACCA
  GTATGTGGCGCAATTTCGTCATATAATCATCCTGAAGCCGATATCGGACCACGTATTCAA
  GGTACGCTTATTAAGAAGCAAGTAATGATGAGAGGCTTCTTGGTAAGTGAATTCGCTAAT
  GCTTTTAAAGAAGCTAGTGAACAGCTTGCAACATGGGTTCAAGAAGGTAAAATTCAGTCA
  CAAGTAACCATTGAAGATGGTTTTGAAAATGCGCCACATGCATTTAAAAATTTATTTACT
  GGCGATAACTTTGGTAAACAAGTTATTAAAGTAACGGAATAA
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⊟Protein

⊟General

• locus tag: NWMN_2091 [new locus tag: NWMN_RS12105 ]
• symbol: NWMN_2091
• description: hypothetical protein
• length: 333
• theoretical pI: 5.08506
• theoretical MW: 36266.2
• GRAVY: -0.0948949

⊟Function

• TIGRFAM:
  leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 267.3)
  and 6 more
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 86.4)
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 44.5)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 34.2)
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 22.6)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.4)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 12.7)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 117.7)
  and 3 more
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 72.5)
  ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 52.5)
  no clan defined Cas_VVA1548; Putative CRISPR-associated protein (Cas_VVA1548) (PF09652; HMM-score: 14.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007734
  • TAT(Tat/SPI): 0.000251
  • LIPO(Sec/SPII): 0.000483
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222303 NCBI
• RefSeq: YP_001333125 NCBI
• UniProt: A0A0H3KI57 UniProt

⊟Protein sequence

• MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYISVDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINHVQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSVVGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYFENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVSEFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications