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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2178 [new locus tag: SACOL_RS11460 ]
  • pan locus tag?: SAUPAN005583000
  • symbol: SACOL2178
  • pan gene symbol?: —
  • synonym:
  • product: alcohol dehydrogenase, zinc-containing

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2178 [new locus tag: SACOL_RS11460 ]
  • symbol: SACOL2178
  • product: alcohol dehydrogenase, zinc-containing
  • replicon: chromosome
  • strand: -
  • coordinates: 2260085..2261086
  • length: 1002
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAAATAAACAAATTCTTTTTAACAAAATACCAGAAGGCATGCCTCAAGAGGATACA
    TTCAAAATTGAAGAGATTGACACGCCTAAACTTGAGAGCGATGGTGTACTTGTACAAACG
    CTGTATATTTCAGTGGATCCATATATGAGAGGACGTATGACTAAGGCAGATTCGTATGTA
    CAGCCTTTTGAAATTGGTAAACCGATTGTTAGCCATGTCGTTGCAAAAGTAATTGATTCG
    ACACTCGCAGATTATAAAAAAGGAGATGTCGTTGTAGGTATGTTGCCTTGGCGAATCATT
    AATCATGTCCAAGCGGATCAAATTACTAAAGTTCCAACGACAGATGTACCACTTGATTTA
    TATTTAAGTGTTTTAGGAATGCCAGGACAAACAGCATATCATGGTCTGCTTGATATTGGA
    CAACCTAAAGCCGGTGATACTGTTGTGGTTTCAGCTGCATCTGGTGCTGTAGGTTCAGTT
    GTAGGTCAAATTGCTAAGATTAAAGGTTGCCGTGTAGTAGGTATTGCGGGTGGCGACAAA
    AAAGTAAATTATTTAACAGAGACACTGGGCTTTGACGCTGGTATTGATTATAAGAAAGAG
    GATTTTGCAGAAGCGTTAGCACAAGCAGTACCAGATGGTATTGATGTTTATTTTGAAAAT
    GTTGGTGGAACAGTTGGCGATGAAGTATTTAAGCATTTAAATCGTTTTGCACGTGTACCA
    GTATGTGGCGCAATTTCGTCATATAATCATCCTGAAGCCGATATCGGACCACGTATTCAA
    GGTACGCTTATTAAGAAGCAAGTAATGATGAGAGGCTTCTTGGTAAGTGAATTCGCTAAT
    GCTTTTAAAGAAGCTAGTGAACAGCTTGCAACATGGGTTCAAGAAGGTAAAATTCAGTCA
    CAAGTAACCATTGAAGATGGTTTTGAAAATGCGCCACATGCATTTAAAAATTTATTTACT
    GGCGATAACTTTGGTAAACAAGTTATTAAAGTAACGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2178 [new locus tag: SACOL_RS11460 ]
  • symbol: SACOL2178
  • description: alcohol dehydrogenase, zinc-containing
  • length: 333
  • theoretical pI: 5.08506
  • theoretical MW: 36266.2
  • GRAVY: -0.0948949

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.1?  ExPASy
    Alcohol dehydrogenase A primary alcohol + NAD+ = an aldehyde + NADH A secondary alcohol + NAD+ = a ketone + NADH
  • TIGRFAM:
    leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 267.3)
    and 6 more
    Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 86.4)
    Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 44.5)
    crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 34.2)
    Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 22.6)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.4)
    Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 12.7)
  • TheSEED  :
    • Putative oxidoreductase YncB
    Miscellaneous Miscellaneous - no subcategory Broadly distributed proteins not in subsystems  Putative oxidoreductase YncB
  • PFAM:
    GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 117.7)
    and 3 more
    NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 72.5)
    ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 52.5)
    no clan defined Cas_VVA1548; Putative CRISPR-associated protein (Cas_VVA1548) (PF09652; HMM-score: 14.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007734
    • TAT(Tat/SPI): 0.000251
    • LIPO(Sec/SPII): 0.000483
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYISVDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINHVQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSVVGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYFENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVSEFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 758 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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