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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2181 [new locus tag: NWMN_RS12600 ]
  • pan locus tag?: SAUPAN005743000
  • symbol: NWMN_2181
  • pan gene symbol?: —
  • synonym:
  • product: acetyltransferase, GNAT family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2181 [new locus tag: NWMN_RS12600 ]
  • symbol: NWMN_2181
  • product: acetyltransferase, GNAT family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2403462..2404238
  • length: 777
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGTCAATGGCTAGTATAAGTATGTCAGATATATATTGTAACGGCACTATATTTGAAAAT
    GACGACGAGCAGTTGATTTATTTAACGCCTTCTTTTCCACAACGATACACAAGTAACACA
    TGGATATATAAAAAGACGCCTACCCAAGAGCGATGGCTGAAAGACTTAGAACGTCAACAT
    CAATTACATACAAATCAAGGTTCAAATCATTATGCGTTTAGTTTCCCGGAAAATGAACAA
    CTTGATAATCATTGGATGGCTATGTTTAAAGATATGAATTTTGAACTAGGTATTATGGAA
    TTGTATGCCATAGAAAGTGATGCGCTTGCCAATTTGCCGCGTAACTCTGACGTTGAAATT
    GCCATCGTTGACGAGTCGCATATAGATGCCTATTTAAAAGTTGCATATCAGTTTAGTTTG
    CCATTTGGAAAAGACTATGCAGATGCACATGAAGAAATGGTAAGGGAACATTATCAAAAA
    GATGTGATTAAACGCTTAGTAGCTTATTTAAATAATGAACCTATTGGCGTTGTAGATGTC
    ATTGAAAGTGAAAATTACATTGAATTAGATGGATTTGGTGTATTAGAACAATTTCGGCAC
    CAAGGAATTGGATCTACAATTCAATCGTTGATAGGTGAATACGCCATATCAAAAAATCAC
    AAACCAATCATATTAGTTGCAGATGGTGAAGATACAGCAAAAGATATGTATGCAAAGCAA
    GGTTATGTCTATCAATCGTTTTGTTATCAAATATTAAAAGAAGATATTGGAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    777

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2181 [new locus tag: NWMN_RS12600 ]
  • symbol: NWMN_2181
  • description: acetyltransferase, GNAT family protein
  • length: 258
  • theoretical pI: 4.39872
  • theoretical MW: 29886.3
  • GRAVY: -0.460853

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal-protein-alanine acetyltransferase (TIGR01575; EC 2.3.1.128; HMM-score: 16)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Acetyltrans (CL0257) Acetyltransf_10; Acetyltransferase (GNAT) domain (PF13673; HMM-score: 31.1)
    Acetyltransf_1; Acetyltransferase (GNAT) family (PF00583; HMM-score: 29.1)
    Acetyltransf_7; Acetyltransferase (GNAT) domain (PF13508; HMM-score: 28)
    and 2 more
    FR47; FR47-like protein (PF08445; HMM-score: 20.5)
    no clan defined XRCC4; DNA double-strand break repair and V(D)J recombination protein XRCC4 (PF06632; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012445
    • TAT(Tat/SPI): 0.000418
    • LIPO(Sec/SPII): 0.00393
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSMASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKTPTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAIESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDVIKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNHKPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA (repression) regulon
    BirA(TF)important in Biotin biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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