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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2197 [new locus tag: NWMN_RS12680 ]
  • pan locus tag?: SAUPAN005763000
  • symbol: sarY
  • pan gene symbol?: sarY
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2197 [new locus tag: NWMN_RS12680 ]
  • symbol: sarY
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2416379..2417122
  • length: 744
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCTAAATTTAAAGTTAAGCGTTTAACTGCACACATACTAATGTTGAAAGTCATTAAT
    GATATGTTGAAATATTCATTTCATCTCACTTTGACTCAAGTGAAACTATTAAATCTATTA
    GTTAAATATGATAATTCTGATTCCAATAGCGCTTCAATAGATAGTTTATTAAAAATGAAA
    GAAATCCAATCTAAGATGGCCCTTCTTCAAATGTTAAGTTATTTAAACAATCATCAATGG
    CTATTGAAAGGAAGAGATCAAAGGGATCAACGTAAATTGACTATTTCGATTAATAAAATG
    CATATAGATAAAATAGAATATATGAATAATGAGCTTAGTGACTATATTGAACGTTATTTT
    GGTCAATATACATTTGATTTTAGTTGTGACTATGTATCGTATTTATTGTCGTCACAAGAA
    TTATTGCGGAATCTAAAGTGTTATTTAAATATGTGTCAATTATCATTAGAAGAGTTATAC
    GTTTTAGGAATATTAAATCTGCATAAAGGTCAATTGACGGTTAAAGAATTACAGGGTGAA
    TTCCATCACCCGATATTTGCGGTTAGCCCGATTTTAAAGTGTTTAATTTTGAAGGGATTA
    GTTAAAAAAGAGAGATGTGAATTAGATGAAAGGCGTGTTATTGTAACAATCAAGCGAGAA
    AAGTTTTCTAAAGTCACTATGCTTATTCAAGCATGTTATAATTATCTTGAAAAGGGAATA
    CAAGTTAATCTTAATAATAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    744

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2197 [new locus tag: NWMN_RS12680 ]
  • symbol: SarY
  • description: hypothetical protein
  • length: 247
  • theoretical pI: 9.58575
  • theoretical MW: 29143.2
  • GRAVY: -0.184211

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 198.4)
    and 1 more
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 25.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HTH (CL0123) MarR_2; MarR family (PF12802; HMM-score: 24.9)
    MarR; MarR family (PF01047; HMM-score: 20.3)
    and 2 more
    RING (CL0229) zf-C3HC4_5; Zinc finger, C3HC4 type (RING finger) (PF17121; HMM-score: 13.3)
    HTH (CL0123) TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009089
    • TAT(Tat/SPI): 0.000213
    • LIPO(Sec/SPII): 0.000468
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYDNSDSNSASIDSLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHIDKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELLRNLKCYLNMCQLSLEELYVLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIKREKFSKVTMLIQACYNYLEKGIQVNLNNK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB (activation) regulon
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [1]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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