PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2227 [new locus tag: NWMN_RS12840 ]
• pan locus tag^?: SAUPAN005807000
• symbol: NWMN_2227
• pan gene symbol^?: —
• synonym:
• product: Na+/H+ antiporter family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2227 [new locus tag: NWMN_RS12840 ]
• symbol: NWMN_2227
• product: Na+/H+ antiporter family protein
• replicon: chromosome
• strand: -
• coordinates: 2447898..2449277
• length: 1380
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331351 NCBI
• RefSeq: YP_001333261 NCBI
• BioCyc:
• MicrobesOnline: 3707829 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAACCTCTTTCTTCATCGTTTGTGAGAAAGAGGTATTTTTAATTGGAAAGCAGGTA
  AAAAGGATGGAAGTACATAAAAAGAGCAATGCTTGGGCATTATTCCCCTTGTTATTATTT
  GTGGCGTTGTTTTTAGGCGTAGGTATTATCACAGGTGATTTTACTTCAATGCCATTAAAT
  GTTGCAATTACGATAACGGTAATTGTGGCGTTATTAATGAATCGAAAAGAATCATTTGCT
  AAAAAAGTTGAGGTCTTCACGAAAGGTGCAGGCCATTCAAACATTGTTTTAATGATGTTG
  ATTTTTATTTTAGCAGGTGCATTTTCAAATACAGCTGAAAAAATGGGTGGCGTAAAGTCG
  ACAGTTAATTTAGGACTATCATTAATCCCAGAAAATTTAATTATTGTAGGATTGTTTGTG
  ATTTGTATGTTTGTTTCTATATCTATGGGGACGTCTGTAGGAACTGTTGCTGCAATAGCA
  CCTGTTGGTTACGGATTTGCACAAGCGACAGATGTACCAACTGCGCTAGCAATGGCTACA
  GTTGTTGGAGGTGCAATGTTCGGCGATAATCTATCAATGATTTCAGATACAACAATTGCT
  GCGGTTAGAACACAACATACGAAAATGAAAGATAAATTCAGAGTAAACTTTAAAATTGTA
  TTGCCAGGTGCTATTTTAACGATTATTATTCTGTATTTCTTAACGAATGGCATTTCTTTA
  AATCATGCTAAAAACTATGATTATAATCTAATTAAAGTTGTACCGTATATCTTAGTATTG
  GTATTAGCATTATTAGGTGTAAATGTCATTATCGTATTGATTGGCGGAACATTATTAGCC
  GGAATCATTGGTCTCATAGATGGTTCATTCGGTTGGATGGGGCTACTAGATGCAGTGTCC
  AAAGGTATTATAAGCATGGAAGATATTGCTATGATTGCGTTGCTAATTGGTGGTTTAGTC
  GGTATTATTCAACACAATGGTGGTATTGAATGGTTGTTGCAATTTGTTCGCTCTAAAGTG
  AAATCAAAGCGTGGCGCAGAATTAGGAATTGCTAGTTTGGTAAGTGTCGCAGATATTGCC
  ACGGCAAACAACACCATCTCTATTATTATGTCAGGTCCTTTAGCTAAAAATATTGCAGAT
  GAATATGATGTAGATTCAAGGAAATCAGCAAGTATTCTTGATATATTTGGAGGCTGCTTC
  CAAGGTTTATTACCATATAGCCCGCAAGTCATTTCAGCGGCTGGTGTAGCTGGTATTTCA
  CCGTTAATGTTATTCCCATACAGCATTTATCCTATATTATTAGGTGTATGCGGACTTATT
  GCGATTTTATTTAATTTTCCAAAGCTAAAAAAGAACACTAGTCAAGAAATGAAACATTAA
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⊟Protein

⊟General

• locus tag: NWMN_2227 [new locus tag: NWMN_RS12840 ]
• symbol: NWMN_2227
• description: Na+/H+ antiporter family protein
• length: 459
• theoretical pI: 9.66495
• theoretical MW: 48937.3
• GRAVY: 0.820261

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds Na+/H+ antiporter NhaC (TIGR00931; HMM-score: 86.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 80.4)
  and 3 more
  Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 21.1)
  DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 18.8)
  CitMHS; Citrate transporter (PF03600; HMM-score: 15.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 10
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009937
  • TAT(Tat/SPI): 0.000209
  • LIPO(Sec/SPII): 0.006541
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: 151222439 NCBI
• RefSeq: YP_001333261 NCBI
• UniProt: A0A0H3KA52 UniProt

⊟Protein sequence

• MKTSFFIVCEKEVFLIGKQVKRMEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: S-box (transcription termination) regulon

  S-box

  (RNA)

  important in Methionine biosynthesis; regulatory site identified based on RegPrecise data for N315 RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications