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NCBI: 01-MAR-2011 (discontinued in NCBI 03-AUG-2016)

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02601
  • pan locus tag?: SAUPAN005807000
  • symbol: SAOUHSC_02601
  • pan gene symbol?: —
  • synonym:
  • product: Na+/H+ antiporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02601
  • symbol: SAOUHSC_02601
  • product: Na+/H+ antiporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2390182..2391393
  • length: 1212
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCCATTAAATGTTGCAATTACGATAACGGTAATTGTGGCGTTATTAATGAATCGAAAA
    GAATCATTTGCTAAAAAAGTTGAGGTCTTCACGAAAGGTGCAGGCCATTCAAACATTGTT
    TTAATGATGTTGATTTTTATTTTAGCAGGTGCATTTTCAAATACAGCTGAAAAAATGGGT
    GGCGTAAAGTCGACAGTTAATTTAGGACTATCATTAATCCCAGAAAATTTAATTATTGTA
    GGATTGTTTGTGATTTGTATGTTTGTTTCTATATCTATGGGGACGTCTGTAGGAACTGTT
    GCTGCAATAGCACCTGTTGGTTACGGATTTGCACAAGCGACAGATGTACCAACTGCGCTA
    GCAATGGCTACAGTTGTTGGAGGTGCAATGTTCGGCGATAATCTATCAATGATTTCAGAT
    ACAACAATTGCTGCGGTTAGAACACAACATACGAAAATGAAAGATAAATTCAGAGTAAAC
    TTTAAAATTGTATTGCCAGGTGCTATTTTAACGATTATTATTCTGTATTTCTTAACGAAT
    GGCATTTCTTTAAATCATGCTAAAAACTATGATTATAATCTAATTAAAGTTGTACCGTAT
    ATCTTAGTATTGGTATTAGCATTATTAGGTGTAAATGTCATTATCGTATTGATTGGCGGA
    ACATTATTAGCCGGAATCATTGGTCTCATAGATGGTTCATTCGGTTGGATGGGGCTACTA
    GATGCAGTGTCCAAAGGTATTATAAGCATGGAAGATATTGCTATGATTGCGTTGCTAATT
    GGTGGTTTAGTCGGTATTATTCAACACAATGGTGGTATTGAATGGTTGTTGCAATTTGTT
    CGCTCTAAAGTGAAATCAAAGCGTGGCGCAGAATTAGGAATTGCTAGTTTGGTAAGTGTC
    GCAGATATTGCCACGGCAAACAACACCATCTCTATTATTATGTCAGGTCCTTTAGCTAAA
    AATATTGCAGATGAATATGATGTAGATTCAAGGAAATCAGCAAGTATTCTTGATATATTT
    GGAGGCTGCTTCCAAGGTTTATTACCATATAGCCCGCAAGTCATTTCAGCGGCTGGTGTA
    GCTGGTATTTCACCGTTAATGTTATTCCCATACAGCATTTATCCTATATTATTAGGTGTA
    TGCGGACTTATTGCGATTTTATTTAATTTTCCAAAGCTAAAAAAGAACACTAGTCAAGAA
    ATGAAACATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1212

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02601
  • symbol: SAOUHSC_02601
  • description: Na+/H+ antiporter
  • length: 403
  • theoretical pI: 9.42314
  • theoretical MW: 42605.7
  • GRAVY: 0.833995

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds Na+/H+ antiporter NhaC (TIGR00931; HMM-score: 87.2)
  • TheSEED  :
    • Methionine transporter MetT
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Methionine transporter MetT
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Methionine transporter MetT
  • PFAM:
    IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 80.5)
    and 4 more
    CitMHS; Citrate transporter (PF03600; HMM-score: 22.9)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 21.4)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 11.9)
    GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 8.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.067531
    • TAT(Tat/SPI): 0.002184
    • LIPO(Sec/SPII): 0.012929
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPLNVAITITVIVALLMNRKESFAKKVEVFTKGAGHSNIVLMMLIFILAGAFSNTAEKMGGVKSTVNLGLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVVGGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISLNHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDAVSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSVADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAAGVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: S-box (transcription termination) regulon
    S-box(5' cis-acting region)important in Methionine biosynthesis; compare RegPrecise for N315 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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